Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SIO17_RS18705 Genome accession   NZ_CP139436
Coordinates   4119898..4121019 (+) Length   373 a.a.
NCBI ID   WP_010379027.1    Uniprot ID   -
Organism   Pseudoalteromonas piscicida strain WJX-06     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4114898..4126019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIO17_RS18675 (SIO17_18675) - 4115699..4116292 (-) 594 WP_010379017.1 XTP/dITP diphosphatase -
  SIO17_RS18680 (SIO17_18680) - 4116300..4116734 (-) 435 WP_010379019.1 DUF4426 domain-containing protein -
  SIO17_RS18685 (SIO17_18685) - 4116745..4117278 (-) 534 WP_010379021.1 YggT family protein -
  SIO17_RS18690 (SIO17_18690) proC 4117288..4118109 (-) 822 WP_010379022.1 pyrroline-5-carboxylate reductase -
  SIO17_RS18695 (SIO17_18695) - 4118123..4118806 (-) 684 WP_010379024.1 YggS family pyridoxal phosphate-dependent enzyme -
  SIO17_RS18700 (SIO17_18700) pilT 4118837..4119883 (+) 1047 WP_010379026.1 type IV pilus twitching motility protein PilT Machinery gene
  SIO17_RS18705 (SIO17_18705) pilU 4119898..4121019 (+) 1122 WP_010379027.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SIO17_RS18710 (SIO17_18710) - 4120997..4121884 (+) 888 WP_010379030.1 hypothetical protein -
  SIO17_RS18715 (SIO17_18715) ruvX 4122189..4122638 (-) 450 WP_010379033.1 Holliday junction resolvase RuvX -
  SIO17_RS18720 (SIO17_18720) - 4122681..4123238 (-) 558 WP_010379034.1 YqgE/AlgH family protein -
  SIO17_RS18725 (SIO17_18725) gshB 4123258..4124217 (-) 960 WP_010379037.1 glutathione synthase -
  SIO17_RS18730 (SIO17_18730) rsmE 4124275..4125009 (-) 735 WP_010379040.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SIO17_RS18735 (SIO17_18735) - 4125072..4125569 (-) 498 WP_010379041.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41195.08 Da        Isoelectric Point: 6.0556

>NTDB_id=909712 SIO17_RS18705 WP_010379027.1 4119898..4121019(+) (pilU) [Pseudoalteromonas piscicida strain WJX-06]
MNIEPFLQTMANQQASDLFVSAGLAVSAKIDGELRALSEESLDAEQSLNLVTSIMSDKQKQEFFNTKECNFAIANDIGRF
RVSAFWQRDCAGMVIRRIVTAIPDVNELGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIEDP
IEFVHQHQKSIITQREVGIDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRIM
HLVPKEKHDKLKYDLALNLRAIVAQQLVPSSKGEGREAAIEVLLNFPLVAELIKKGDIGSIKEAMAKSKDMGMQTFDQAL
FELYKQQRINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTVDGLDSKN

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=909712 SIO17_RS18705 WP_010379027.1 4119898..4121019(+) (pilU) [Pseudoalteromonas piscicida strain WJX-06]
ATGAATATAGAACCGTTTTTGCAAACAATGGCCAATCAGCAAGCATCCGACTTATTCGTCTCGGCAGGGCTTGCGGTGAG
CGCCAAAATTGATGGCGAACTGAGAGCGTTAAGTGAAGAAAGCTTAGATGCCGAGCAGTCACTGAACCTTGTTACCTCAA
TTATGAGCGACAAGCAAAAGCAAGAATTCTTTAACACTAAAGAGTGTAACTTTGCGATTGCTAACGATATTGGGCGTTTT
CGTGTTTCAGCATTTTGGCAACGGGATTGTGCGGGCATGGTTATCCGCCGTATCGTTACTGCTATTCCAGATGTCAATGA
GCTCGGTTTGCCGTCGGTCCTGACCGATGTGATTATGTCCAAACGTGGCTTGGTGTTATTTGTGGGTGGTACGGGCACGG
GTAAGTCAACCTCGCTTGCGGCACTACTCGGGTATCGTAATCGTAATCAACGTGGCCATATTTTGACCATTGAAGATCCT
ATTGAGTTTGTGCATCAACACCAAAAAAGCATCATTACTCAGCGTGAAGTGGGCATTGATACTGACAGTTTCGAATCTGC
CTTGAAAAGCTCATTACGCCAAGCGCCAGATGTTATTTTAATTGGTGAGATTCGCTCGCAAGAAACCATGGAATATGCAT
TGAGTTTTGCTGAAACGGGGCATTTATGCGTTGCAACTTTACATGCCAATAATGCCAATCAGGCCATCGACCGTATCATG
CACTTAGTTCCTAAAGAAAAGCACGATAAACTGAAGTACGATTTGGCGCTGAACTTGCGCGCTATTGTGGCGCAACAACT
TGTGCCTTCTTCAAAAGGTGAAGGTCGAGAGGCGGCCATTGAAGTACTACTGAACTTCCCACTCGTTGCCGAACTTATTA
AAAAAGGCGATATTGGCTCAATTAAAGAGGCAATGGCAAAATCGAAAGACATGGGCATGCAAACCTTTGATCAAGCGTTG
TTTGAGCTCTATAAACAACAGCGGATCAATTATGCCGATGCACTACATCATGCTGACTCGCCAAATGATCTGCGCCTCAT
GATCAAGCTTCAAAATAACGAACAAAAAGGTGCGGGCTTCCTACAAGGGGTTACGGTAGATGGGTTGGATTCGAAGAACT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

56.764

100

0.574
  pilU Pseudomonas stutzeri DSM 10701

60.399

94.102

0.568
  pilU Vibrio cholerae strain A1552

55.858

98.391

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.5

96.515

0.41
  pilT Acinetobacter baylyi ADP1

40.936

91.689

0.375
  pilT Legionella pneumophila strain ERS1305867

41.742

89.276

0.373
  pilT Legionella pneumophila strain Lp02

41.742

89.276

0.373
  pilT Pseudomonas aeruginosa PAK

40.29

92.493

0.373
  pilT Acinetobacter nosocomialis M2

40.351

91.689

0.37
  pilT Acinetobacter baumannii D1279779

40.351

91.689

0.37
  pilT Acinetobacter baumannii strain A118

40.351

91.689

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.42

92.493

0.365