NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	SPM78_RS20535	Genome accession	NZ_CP139424
Coordinates	4355649..4356101 (-)	Length	150 a.a.
NCBI ID	WP_031636800.1	Uniprot ID	P17837
Organism	Pseudomonas aeruginosa strain 136790
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4350649..4361101

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPM78_RS20515 (SPM78_20515)	ampD	4351344..4351910 (-)	567	WP_023096121.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
SPM78_RS20520 (SPM78_20520)	-	4352061..4354316 (-)	2256	WP_003104913.1	DUF1631 domain-containing protein	-
SPM78_RS20525 (SPM78_20525)	nadC	4354602..4355450 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
SPM78_RS20535 (SPM78_20535)	pilA	4355649..4356101 (-)	453	WP_031636800.1	pilin	Machinery gene
SPM78_RS20540 (SPM78_20540)	pilB	4356333..4358033 (+)	1701	WP_023094327.1	type IV-A pilus assembly ATPase PilB	Machinery gene
SPM78_RS20545 (SPM78_20545)	pilC	4358037..4359257 (+)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
SPM78_RS20550 (SPM78_20550)	pilD	4359261..4360133 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
SPM78_RS20555 (SPM78_20555)	coaE	4360130..4360741 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
SPM78_RS20560 (SPM78_20560)	yacG	4360738..4360938 (+)	201	WP_017148551.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 15743.11 Da Isoelectric Point: 8.4742

                                >NTDB_id=909621 SPM78_RS20535 WP_031636800.1 4355649..4356101(-) (pilA) [Pseudomonas aeruginosa strain 136790]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV

GIDEKANKLGTVAVTIKDTGDGTVKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide

Download Length: 453 bp

                                >NTDB_id=909621 SPM78_RS20535 WP_031636800.1 4355649..4356101(-) (pilA) [Pseudomonas aeruginosa strain 136790]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT

TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA

GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC

TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAAGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT

GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	P17837

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	66.447	100	0.673
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	45	100	0.48
pilA	Vibrio cholerae strain A1552	45	100	0.48
pilA	Vibrio cholerae C6706	45	100	0.48
pilA/pilAI	Pseudomonas stutzeri DSM 10701	46	100	0.46
pilA	Acinetobacter baumannii strain A118	45.27	98.667	0.447
pilA	Vibrio parahaemolyticus RIMD 2210633	42.568	98.667	0.42
pilA/pilAII	Pseudomonas stutzeri DSM 10701	43.357	95.333	0.413
comP	Acinetobacter baylyi ADP1	41.333	100	0.413
pilA/pilA1	Eikenella corrodens VA1	39.865	98.667	0.393
pilA2	Legionella pneumophila str. Paris	38.255	99.333	0.38
pilA2	Legionella pneumophila strain ERS1305867	38.255	99.333	0.38

Detailed information NTG0909621