Detailed information NTG0905855

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SIC19_RS06985
• Genome accession: NZ_CP138916
• Coordinates: 1499592..1500713 (-)
• Length: 373 a.a.
• NCBI ID: WP_041332635.1
• Uniprot ID: W5YPF4
• Organism: Marinobacter sp. MIT930201
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1494592..1505713

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SIC19_RS06970	polA	1494715..1497450 (+)	2736	WP_126810648.1	DNA polymerase I	-
SIC19_RS06975	-	1497672..1498040 (+)	369	WP_041332636.1	hypothetical protein	-
SIC19_RS06980	-	1498070..1499476 (-)	1407	WP_185906464.1	OmpP1/FadL family transporter	-
SIC19_RS06985	pilU	1499592..1500713 (-)	1122	WP_041332635.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SIC19_RS06990	pilT	1500888..1501922 (-)	1035	WP_027830795.1	type IV pilus twitching motility protein PilT	Machinery gene
SIC19_RS06995	-	1502068..1502760 (+)	693	WP_041332633.1	YggS family pyridoxal phosphate-dependent enzyme	-
SIC19_RS07000	proC	1502844..1503677 (+)	834	WP_036203143.1	pyrroline-5-carboxylate reductase	-
SIC19_RS07005	-	1503701..1504282 (+)	582	WP_036203140.1	YggT family protein	-
SIC19_RS07010	-	1504526..1505287 (+)	762	Protein_1361	dynamin family protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41497.65 Da
• Isoelectric Point: 6.3471

>NTDB_id=905855 SIC19_RS06985 WP_041332635.1 1499592..1500713(-) (pilU) [Marinobacter sp. MIT930201]
MEFEKLLRLMVDKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRVEFEESHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLALPEIIKDLAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLKAIVAQQLIPTPDGQGRKAVIEVLINTPLVADLIRKGEVHRLKELMSKSNESGMRTFDQA
LYELYAEGSITYEDALAHADSANDLRLMIKLGADSQGADKLASSVDKLSIQDD

Nucleotide

• Length: 1122 bp

>NTDB_id=905855 SIC19_RS06985 WP_041332635.1 1499592..1500713(-) (pilU) [Marinobacter sp. MIT930201]
ATGGAATTCGAAAAACTGCTCCGTTTGATGGTGGACAAAGGCGGCTCCGACCTTTTCATTACTGCCGGTGTGCCACCCTC
CATGAAGGTCAACGGCAAGGTCCTGCCTGTCACCAAGAATGCATTGACGCCGGAGCAGACGAGGGAGTTCGTCTATGGCG
CCATGAACGACAAGCAGCGGGTCGAATTCGAAGAAAGCCACGAGTGTAACTTCGCCATCAGTGCCCGAGGCATCGGTCGC
TTCCGTGTCAGCGCCTTCTTCCAGCGCAACCTCTGCGGCATGGTCCTGCGGCGGATCGAGGTGAAAATCCCGCAGATTGA
TGACCTGGCACTGCCGGAAATCATCAAGGATCTGGCCATGACCAAGCGTGGCCTGATCATGTTCGTGGGTGCCACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCTATGCTCGGGCATCGTAACCGCAACAGCCGGGGCCACATTATCTCCATCGAAGAC
CCCATCGAATTCGTGCACCAGCACCAGGGCTGTATCGTGACTCAGCGAGAGGTGGGCATCGATACCGATAGCTTTGAAGT
GGCTCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTCATCGGTGAGGTGCGTACCCGCCAGACCATGGAATACT
CTGTGCAGTTTGCTGAAACCGGCCACCTGTGCCTCGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATCCAGTTCTTCCCGCCGGAACAGCACAACCAGATCTGGATGGACCTGTCCCTGAACCTCAAGGCCATCGTCGCTCAACA
GTTGATCCCGACGCCGGACGGGCAGGGCCGCAAAGCCGTCATTGAAGTGTTGATCAACACACCGTTGGTCGCGGACCTGA
TTCGAAAAGGCGAAGTGCACCGACTTAAGGAACTGATGTCCAAGTCCAACGAGAGCGGCATGCGCACCTTTGATCAGGCG
CTTTATGAGCTCTATGCCGAAGGCTCCATCACTTATGAGGATGCCCTCGCGCACGCGGATTCAGCCAACGACCTGCGCCT
GATGATCAAGCTGGGTGCCGATTCCCAGGGCGCTGACAAGCTGGCTTCCAGTGTCGACAAGTTGTCGATCCAGGACGATT
GA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (114-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	78.437	99.464	0.78
pilU	Acinetobacter baylyi ADP1	63.243	99.196	0.627
pilU	Vibrio cholerae strain A1552	57.429	93.834	0.539
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	90.885	0.394
pilT	Acinetobacter baumannii strain A118	41.194	89.812	0.37
pilT	Acinetobacter baumannii D1279779	41.194	89.812	0.37
pilT	Acinetobacter nosocomialis M2	40.896	89.812	0.367
pilT	Pseudomonas aeruginosa PAK	40.597	89.812	0.365
pilT	Legionella pneumophila strain Lp02	40.299	89.812	0.362
pilT	Legionella pneumophila strain ERS1305867	40.299	89.812	0.362
pilT	Acinetobacter baylyi ADP1	39.589	91.421	0.362