Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RZE77_RS24815 Genome accession   NZ_CP138513
Coordinates   5307218..5308354 (+) Length   378 a.a.
NCBI ID   WP_124958857.1    Uniprot ID   -
Organism   Variovorax paradoxus strain SPNA7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5302218..5313354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE77_RS24800 (RZE77_24800) - 5304916..5305389 (+) 474 WP_319439698.1 Lrp/AsnC ligand binding domain-containing protein -
  RZE77_RS24805 (RZE77_24805) - 5305396..5306094 (-) 699 WP_319439699.1 YggS family pyridoxal phosphate-dependent enzyme -
  RZE77_RS24810 (RZE77_24810) pilT 5306146..5307189 (+) 1044 WP_015867574.1 type IV pilus twitching motility protein PilT Machinery gene
  RZE77_RS24815 (RZE77_24815) pilU 5307218..5308354 (+) 1137 WP_124958857.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZE77_RS24820 (RZE77_24820) - 5308398..5309306 (+) 909 WP_319427908.1 NAD(P)-dependent oxidoreductase -
  RZE77_RS24825 (RZE77_24825) - 5309343..5310110 (-) 768 WP_319439700.1 BON domain-containing protein -
  RZE77_RS24830 (RZE77_24830) - 5310107..5310751 (-) 645 WP_319439701.1 SIS domain-containing protein -
  RZE77_RS24835 (RZE77_24835) - 5310834..5311205 (-) 372 WP_015867579.1 YraN family protein -
  RZE77_RS24840 (RZE77_24840) rsmI 5311251..5312207 (+) 957 WP_319439702.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  RZE77_RS24845 (RZE77_24845) - 5312217..5313056 (-) 840 WP_319439703.1 IclR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41856.89 Da        Isoelectric Point: 6.4189

>NTDB_id=903273 RZE77_RS24815 WP_124958857.1 5307218..5308354(+) (pilU) [Variovorax paradoxus strain SPNA7]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLVPTEDGQGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=903273 RZE77_RS24815 WP_124958857.1 5307218..5308354(+) (pilU) [Variovorax paradoxus strain SPNA7]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
CACCGCGGACTTTCCGCCCGCAATCAAGGTGGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGGGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCAACCGGCATCGGCCGCTTCCGCGTGAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
GGCCAAGCTGCCCACCATCGACGGCCTGGGCATGCCGCAGGTGCTCAAGGACGTGTCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGACTGGCGCAACGAAAATTCCTACGGC
CACATCGTGACGGTGGAAGACCCGGTCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATTTTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCGGTGGCCTTTGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGAGCCCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGATCTCCCAGCGCCTGGTGCCCACCGAGGACGGCCAGGGCCGCGTGGCGGCGGTGGAAGTGCTGCTGAACA
CGCCGCTCATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACCTTCGAGGACGCCATCCGCAACGCCGA
TTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACGGATCTCTCGGCGGGTACGGAGC
ACTTTGCGATTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Acinetobacter baumannii D1279779

44.828

84.392

0.378
  pilT Acinetobacter baumannii strain A118

44.828

84.392

0.378
  pilT Acinetobacter baylyi ADP1

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

43.161

87.037

0.376
  pilT Legionella pneumophila strain ERS1305867

43.161

87.037

0.376