Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R9X41_RS20330 Genome accession   NZ_CP137854
Coordinates   4426725..4427864 (+) Length   379 a.a.
NCBI ID   WP_318632254.1    Uniprot ID   -
Organism   Xylophilus sp. GOD-11R     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4421725..4432864
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R9X41_RS20305 (R9X41_20305) rocF 4421879..4422787 (+) 909 WP_318632249.1 arginase -
  R9X41_RS20310 (R9X41_20310) - 4422906..4424171 (+) 1266 WP_318632250.1 MFS transporter -
  R9X41_RS20315 (R9X41_20315) - 4424182..4424877 (-) 696 WP_318632251.1 YggS family pyridoxal phosphate-dependent enzyme -
  R9X41_RS20320 (R9X41_20320) pilT 4424924..4425967 (+) 1044 WP_318632252.1 type IV pilus twitching motility protein PilT Machinery gene
  R9X41_RS20325 (R9X41_20325) - 4426046..4426681 (+) 636 WP_318632253.1 cyclic nucleotide-binding domain-containing protein -
  R9X41_RS20330 (R9X41_20330) pilU 4426725..4427864 (+) 1140 WP_318632254.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R9X41_RS20335 (R9X41_20335) - 4428374..4429303 (+) 930 WP_318632255.1 NAD(P)-dependent oxidoreductase -
  R9X41_RS20340 (R9X41_20340) - 4429602..4430921 (-) 1320 WP_318632256.1 phospholipase A -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42448.93 Da        Isoelectric Point: 6.7233

>NTDB_id=900917 R9X41_RS20330 WP_318632254.1 4426725..4427864(+) (pilU) [Xylophilus sp. GOD-11R]
MERDQASKFINDLLKLMISRGGSDMFITADFPPAMKVDGKLTKVSPQPLTAAHTLALARSIMNDRQASEFERTKECNFAI
SPASVGRFRVNAFVQQGKVGMVMRLIPTNIPTMEALRMPEVLKEVVMAKRGLCILVGATGSGKSTTLAAMIDHRNENSYG
HIITIEDPVEFVHPHKNCVVTQREVGLDTDNWDIALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCMATLHANSA
NQAIDRIINFFPEERRQQLLMDISLNLKAMISQRLIPVQDGKGRAAAVEVMLNSPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALFDLYEAHQVTYEDALRNADSVNDLRLQIKLNSQRAKDHQDLSRGTEHLEIV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=900917 R9X41_RS20330 WP_318632254.1 4426725..4427864(+) (pilU) [Xylophilus sp. GOD-11R]
ATGGAACGCGACCAGGCCAGTAAATTCATCAACGACCTGCTCAAGCTGATGATCAGCCGGGGCGGCAGCGACATGTTCAT
CACGGCCGACTTTCCGCCGGCGATGAAGGTCGACGGCAAGCTCACCAAGGTGTCGCCGCAGCCGCTGACCGCAGCCCACA
CGCTGGCGCTGGCGCGCTCCATCATGAACGACCGCCAGGCCTCGGAATTCGAGCGCACCAAGGAATGCAATTTCGCGATC
TCGCCGGCGAGCGTGGGGCGGTTCCGGGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGATGCGGCTGATCCC
CACCAACATTCCGACCATGGAAGCCCTGCGCATGCCCGAGGTGCTCAAGGAGGTGGTGATGGCCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGACCACCGCAACGAGAACTCCTACGGC
CACATCATCACCATCGAGGATCCGGTGGAATTCGTGCATCCGCACAAGAACTGCGTGGTGACCCAGCGCGAGGTGGGGCT
CGATACCGACAACTGGGACATCGCGCTGAAGAACACGCTGCGGCAGGCGCCCGACGTGATCCTGATGGGTGAAATCCGCG
ACCGCGAAACGATGGAGCATGCGATCGCCTTCAGCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCAATCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCCAGCAGCTGCTGATGGACATATCGCTCAACCT
GAAGGCGATGATTTCGCAGCGGCTGATTCCAGTGCAGGACGGCAAGGGCCGCGCCGCCGCGGTGGAGGTGATGCTCAACT
CGCCGCTGATCTCGGACCTGATCTTCAAGGGCGAGGTATCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATCTGTACGAGGCCCACCAGGTGACGTACGAGGATGCGCTGCGCAATGCGGA
TTCGGTGAACGACCTGCGGCTGCAGATCAAGCTCAACAGTCAACGTGCGAAGGACCACCAGGACCTGAGTCGTGGGACCG
AGCACCTGGAGATTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.147

93.404

0.58
  pilU Acinetobacter baylyi ADP1

57.377

96.57

0.554
  pilU Vibrio cholerae strain A1552

54.062

94.195

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

88.918

0.409
  pilT Pseudomonas aeruginosa PAK

43.917

88.918

0.391
  pilT Pseudomonas stutzeri DSM 10701

42.136

88.918

0.375
  pilT Legionella pneumophila strain Lp02

43.478

84.96

0.369
  pilT Legionella pneumophila strain ERS1305867

43.478

84.96

0.369
  pilT Acinetobacter baumannii strain A118

42.947

84.169

0.361
  pilT Acinetobacter nosocomialis M2

42.947

84.169

0.361
  pilT Acinetobacter baumannii D1279779

42.947

84.169

0.361