Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R6U47_RS30730 Genome accession   NZ_CP137561
Coordinates   6547230..6547682 (+) Length   150 a.a.
NCBI ID   WP_128664184.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CPA0053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6542230..6552682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6U47_RS30705 (R6U47_30705) yacG 6542396..6542596 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  R6U47_RS30710 (R6U47_30710) coaE 6542593..6543204 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  R6U47_RS30715 (R6U47_30715) pilD 6543201..6544073 (-) 873 WP_023087804.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R6U47_RS30720 (R6U47_30720) pilC 6544077..6545297 (-) 1221 WP_016253888.1 type 4a pilus biogenesis protein PilC Machinery gene
  R6U47_RS30725 (R6U47_30725) pilB 6545301..6547001 (-) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R6U47_RS30730 (R6U47_30730) pilA 6547230..6547682 (+) 453 WP_128664184.1 pilin Machinery gene
  R6U47_RS30740 (R6U47_30740) nadC 6547870..6548718 (-) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R6U47_RS30745 (R6U47_30745) - 6549004..6551259 (+) 2256 WP_324001937.1 DUF1631 domain-containing protein -
  R6U47_RS30750 (R6U47_30750) ampD 6551410..6551976 (+) 567 WP_023084738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15621.04 Da        Isoelectric Point: 9.0827

>NTDB_id=899135 R6U47_RS30730 WP_128664184.1 6547230..6547682(+) (pilA) [Pseudomonas aeruginosa strain CPA0053]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQNEQFIPKGCSK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=899135 R6U47_RS30730 WP_128664184.1 6547230..6547682(+) (pilA) [Pseudomonas aeruginosa strain CPA0053]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGAATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

99.333

100

0.993
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.02

100

0.473
  pilA Acinetobacter baumannii strain A118

45.333

100

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.675

100

0.447
  pilA Vibrio cholerae strain A1552

42.675

100

0.447
  pilA Vibrio cholerae C6706

42.675

100

0.447
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.89

97.333

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

48.507

89.333

0.433
  comP Acinetobacter baylyi ADP1

40.132

100

0.407
  pilA2 Legionella pneumophila strain ERS1305867

39.865

98.667

0.393
  pilA/pilA1 Eikenella corrodens VA1

39.456

98

0.387
  pilA2 Legionella pneumophila str. Paris

39.189

98.667

0.387