Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R5031_RS24330 Genome accession   NZ_CP137512
Coordinates   5212222..5212674 (+) Length   150 a.a.
NCBI ID   WP_317951468.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA0271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5207222..5217674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5031_RS24305 (R5031_24305) yacG 5207385..5207585 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  R5031_RS24310 (R5031_24310) coaE 5207582..5208193 (-) 612 WP_010793792.1 dephospho-CoA kinase -
  R5031_RS24315 (R5031_24315) pilD 5208190..5209062 (-) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R5031_RS24320 (R5031_24320) pilC 5209066..5210286 (-) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  R5031_RS24325 (R5031_24325) pilB 5210290..5211990 (-) 1701 WP_317951467.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5031_RS24330 (R5031_24330) pilA 5212222..5212674 (+) 453 WP_317951468.1 pilin Machinery gene
  R5031_RS24340 (R5031_24340) - 5212929..5213171 (-) 243 WP_023109353.1 hypothetical protein -
  R5031_RS24345 (R5031_24345) - 5215357..5216505 (+) 1149 WP_317951469.1 DUF6531 domain-containing protein -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15427.70 Da        Isoelectric Point: 9.0758

>NTDB_id=898703 R5031_RS24330 WP_317951468.1 5212222..5212674(+) (pilA) [Pseudomonas aeruginosa strain HPA0271]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTADTTYV
GVDATANKLGTIAVTIGNKGAGTVTFTFAAGNSSPKNSGKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=898703 R5031_RS24330 WP_317951468.1 5212222..5212674(+) (pilA) [Pseudomonas aeruginosa strain HPA0271]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGCAGATACTACCTATGTA
GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAAGGGCGCAGGTACCGTGACTTTCAC
TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGGTAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT
GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.456

99.333

0.68
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.333

100

0.473
  pilA Vibrio parahaemolyticus RIMD 2210633

46.939

98

0.46
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.671

100

0.46
  pilA Vibrio cholerae strain A1552

43.671

100

0.46
  pilA Vibrio cholerae C6706

43.671

100

0.46
  pilA Acinetobacter baumannii strain A118

44.295

99.333

0.44
  comP Acinetobacter baylyi ADP1

41.558

100

0.427
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.611

99.333

0.413
  pilA2 Legionella pneumophila str. Paris

40.94

99.333

0.407