Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R5031_RS24325 Genome accession   NZ_CP137512
Coordinates   5210290..5211990 (-) Length   566 a.a.
NCBI ID   WP_317951467.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA0271     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5205290..5216990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5031_RS24285 (R5031_24285) - 5205455..5205880 (+) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  R5031_RS24290 (R5031_24290) - 5205877..5206347 (+) 471 WP_010793791.1 hypothetical protein -
  R5031_RS24295 (R5031_24295) - 5206344..5207033 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  R5031_RS24300 (R5031_24300) - 5207139..5207348 (+) 210 WP_003094660.1 hypothetical protein -
  R5031_RS24305 (R5031_24305) yacG 5207385..5207585 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  R5031_RS24310 (R5031_24310) coaE 5207582..5208193 (-) 612 WP_010793792.1 dephospho-CoA kinase -
  R5031_RS24315 (R5031_24315) pilD 5208190..5209062 (-) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R5031_RS24320 (R5031_24320) pilC 5209066..5210286 (-) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  R5031_RS24325 (R5031_24325) pilB 5210290..5211990 (-) 1701 WP_317951467.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5031_RS24330 (R5031_24330) pilA 5212222..5212674 (+) 453 WP_317951468.1 pilin Machinery gene
  R5031_RS24340 (R5031_24340) - 5212929..5213171 (-) 243 WP_023109353.1 hypothetical protein -
  R5031_RS24345 (R5031_24345) - 5215357..5216505 (+) 1149 WP_317951469.1 DUF6531 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62301.19 Da        Isoelectric Point: 5.7015

>NTDB_id=898702 R5031_RS24325 WP_317951467.1 5210290..5211990(-) (pilB) [Pseudomonas aeruginosa strain HPA0271]
MNNSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVCFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=898702 R5031_RS24325 WP_317951467.1 5210290..5211990(-) (pilB) [Pseudomonas aeruginosa strain HPA0271]
ATGAACAACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACACACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCTTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCTATCAGCGAGAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGTGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.343

100

0.58
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

50.709

99.647

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

99.293

0.5
  pilB Vibrio cholerae strain A1552

49.823

99.647

0.496
  pilB Vibrio campbellii strain DS40M4

49.468

99.647

0.493
  pilF Thermus thermophilus HB27

39.394

99.117

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.228

93.11

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

38.577

94.346

0.364