Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4705_RS04485 Genome accession   NZ_CP137115
Coordinates   830441..831382 (-) Length   313 a.a.
NCBI ID   WP_050084188.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 20614-6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825441..836382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4705_RS04445 comYH 825927..826880 (-) 954 WP_050095638.1 class I SAM-dependent methyltransferase Machinery gene
  R4705_RS04450 - 826941..827535 (-) 595 Protein_845 class I SAM-dependent methyltransferase -
  R4705_RS04455 comGG/cglG 827672..828085 (-) 414 WP_050084191.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4705_RS04460 comGF/cglF 828063..828524 (-) 462 WP_050084190.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4705_RS04465 comGE/cglE 828487..828789 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4705_RS04470 comGD/cglD 828752..829156 (-) 405 WP_001864568.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4705_RS04475 comGC/cglC 829149..829475 (-) 327 WP_050084189.1 competence type IV pilus major pilin ComGC Machinery gene
  R4705_RS04480 comGB/cglB 829477..830493 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4705_RS04485 comGA/cglA/cilD 830441..831382 (-) 942 WP_050084188.1 competence type IV pilus ATPase ComGA Machinery gene
  R4705_RS04490 - 831459..831824 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4705_RS04495 - 831975..833033 (-) 1059 WP_050084187.1 zinc-dependent alcohol dehydrogenase family protein -
  R4705_RS04500 nagA 833196..834347 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4705_RS04505 - 834500..836317 (-) 1818 WP_001220922.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35605.54 Da        Isoelectric Point: 6.0083

>NTDB_id=896261 R4705_RS04485 WP_050084188.1 830441..831382(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 20614-6]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAIVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=896261 R4705_RS04485 WP_050084188.1 830441..831382(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 20614-6]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGATATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAATTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.722

100

0.987
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361