NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	R4705_RS04460	Genome accession	NZ_CP137115
Coordinates	828063..828524 (-)	Length	153 a.a.
NCBI ID	WP_050084190.1	Uniprot ID	-
Organism	Streptococcus pneumoniae strain 20614-6
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 823063..833524

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4705_RS04425	-	823367..824191 (-)	825	WP_317815598.1	membrane protein insertase YidC	-
R4705_RS04430	rnpA	824166..824537 (-)	372	WP_000739246.1	ribonuclease P protein component	-
R4705_RS04435	-	824554..824685 (-)	132	WP_000768904.1	hypothetical protein	-
R4705_RS04440	-	824686..825876 (-)	1191	WP_000167765.1	acetate kinase	-
R4705_RS04445	comYH	825927..826880 (-)	954	WP_050095638.1	class I SAM-dependent methyltransferase	Machinery gene
R4705_RS04450	-	826941..827535 (-)	595	Protein_845	class I SAM-dependent methyltransferase	-
R4705_RS04455	comGG/cglG	827672..828085 (-)	414	WP_050084191.1	competence type IV pilus minor pilin ComGG	Machinery gene
R4705_RS04460	comGF/cglF	828063..828524 (-)	462	WP_050084190.1	competence type IV pilus minor pilin ComGF	Machinery gene
R4705_RS04465	comGE/cglE	828487..828789 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
R4705_RS04470	comGD/cglD	828752..829156 (-)	405	WP_001864568.1	competence type IV pilus minor pilin ComGD	Machinery gene
R4705_RS04475	comGC/cglC	829149..829475 (-)	327	WP_050084189.1	competence type IV pilus major pilin ComGC	Machinery gene
R4705_RS04480	comGB/cglB	829477..830493 (-)	1017	WP_074196785.1	competence type IV pilus assembly protein ComGB	Machinery gene
R4705_RS04485	comGA/cglA/cilD	830441..831382 (-)	942	WP_050084188.1	competence type IV pilus ATPase ComGA	Machinery gene
R4705_RS04490	-	831459..831824 (-)	366	WP_000286415.1	DUF1033 family protein	-
R4705_RS04495	-	831975..833033 (-)	1059	WP_050084187.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

Download Length: 153 a.a. Molecular weight: 18050.80 Da Isoelectric Point: 9.6092

                                >NTDB_id=896256 R4705_RS04460 WP_050084190.1 828063..828524(-) (comGF/cglF) [Streptococcus pneumoniae strain 20614-6]

MVQNSCWQSKSHKVKAFTLLESLLVLIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN

RFYMKQDGKEIAMGKSKADDFRKTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS

Nucleotide

Download Length: 462 bp

                                >NTDB_id=896256 R4705_RS04460 WP_050084190.1 828063..828524(-) (comGF/cglF) [Streptococcus pneumoniae strain 20614-6]

ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGTCCTCAT

TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG

AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAGTTGGAGGTAGAATTAGATCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT

CGTTTCTATATGAAGCAGGATGGCAAGGAGATTGCTATGGGTAAGTCTAAGGCAGATGATTTCCGTAAAACGAATGCTCG

TGGTCGAGGTTATCAGCCTATGGTTTATGGACTCAAATCTGTACGGATTACAGAGGACAATCAACTGGTTCGCTTTCATT

TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae Rx1	96.732	100	0.967
comGF/cglF	Streptococcus pneumoniae D39	96.732	100	0.967
comGF/cglF	Streptococcus pneumoniae R6	96.732	100	0.967
comGF/cglF	Streptococcus pneumoniae TIGR4	96.732	100	0.967
comGF/cglF	Streptococcus mitis NCTC 12261	88.235	100	0.882
comGF/cglF	Streptococcus mitis SK321	88.235	100	0.882

Detailed information NTG0896256