Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R4705_RS04480 Genome accession   NZ_CP137115
Coordinates   829477..830493 (-) Length   338 a.a.
NCBI ID   WP_074196785.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 20614-6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 824477..835493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4705_RS04435 - 824554..824685 (-) 132 WP_000768904.1 hypothetical protein -
  R4705_RS04440 - 824686..825876 (-) 1191 WP_000167765.1 acetate kinase -
  R4705_RS04445 comYH 825927..826880 (-) 954 WP_050095638.1 class I SAM-dependent methyltransferase Machinery gene
  R4705_RS04450 - 826941..827535 (-) 595 Protein_845 class I SAM-dependent methyltransferase -
  R4705_RS04455 comGG/cglG 827672..828085 (-) 414 WP_050084191.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4705_RS04460 comGF/cglF 828063..828524 (-) 462 WP_050084190.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4705_RS04465 comGE/cglE 828487..828789 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4705_RS04470 comGD/cglD 828752..829156 (-) 405 WP_001864568.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4705_RS04475 comGC/cglC 829149..829475 (-) 327 WP_050084189.1 competence type IV pilus major pilin ComGC Machinery gene
  R4705_RS04480 comGB/cglB 829477..830493 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4705_RS04485 comGA/cglA/cilD 830441..831382 (-) 942 WP_050084188.1 competence type IV pilus ATPase ComGA Machinery gene
  R4705_RS04490 - 831459..831824 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4705_RS04495 - 831975..833033 (-) 1059 WP_050084187.1 zinc-dependent alcohol dehydrogenase family protein -
  R4705_RS04500 nagA 833196..834347 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38392.40 Da        Isoelectric Point: 9.4802

>NTDB_id=896260 R4705_RS04480 WP_074196785.1 829477..830493(-) (comGB/cglB) [Streptococcus pneumoniae strain 20614-6]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEV
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=896260 R4705_RS04480 WP_074196785.1 829477..830493(-) (comGB/cglB) [Streptococcus pneumoniae strain 20614-6]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAGTC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTTAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

100

100

1
  comGB/cglB Streptococcus pneumoniae D39

100

100

1
  comGB/cglB Streptococcus pneumoniae R6

100

100

1
  comGB/cglB Streptococcus pneumoniae TIGR4

100

100

1
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.547

94.083

0.541
  comYB Streptococcus mutans UA159

57.547

94.083

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506