Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4709_RS06445 Genome accession   NZ_CP137102
Coordinates   1261052..1261993 (+) Length   313 a.a.
NCBI ID   WP_000249567.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16H3071-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1256052..1266993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4709_RS06425 - 1256118..1257935 (+) 1818 WP_044792290.1 acyltransferase family protein -
  R4709_RS06430 nagA 1258088..1259239 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4709_RS06435 - 1259402..1260460 (+) 1059 WP_044812807.1 zinc-dependent alcohol dehydrogenase family protein -
  R4709_RS06440 - 1260611..1260976 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4709_RS06445 comGA/cglA/cilD 1261052..1261993 (+) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  R4709_RS06450 comGB/cglB 1261941..1262957 (+) 1017 WP_078132972.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4709_RS06455 comGC/cglC 1262959..1263285 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  R4709_RS06460 comGD/cglD 1263278..1263682 (+) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4709_RS06465 comGE/cglE 1263645..1263947 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4709_RS06470 comGF/cglF 1263910..1264371 (+) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4709_RS06475 comGG/cglG 1264349..1264795 (+) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4709_RS06480 - 1264898..1265492 (+) 595 Protein_1266 class I SAM-dependent methyltransferase -
  R4709_RS06485 comYH 1265553..1266506 (+) 954 WP_044813830.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35573.48 Da        Isoelectric Point: 6.0083

>NTDB_id=895259 R4709_RS06445 WP_000249567.1 1261052..1261993(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16H3071-2]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=895259 R4709_RS06445 WP_000249567.1 1261052..1261993(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16H3071-2]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTTTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361