NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	R4709_RS06470	Genome accession	NZ_CP137102
Coordinates	1263910..1264371 (+)	Length	153 a.a.
NCBI ID	WP_000250539.1	Uniprot ID	-
Organism	Streptococcus pneumoniae strain 16H3071-2
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1258910..1269371

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4709_RS06435	-	1259402..1260460 (+)	1059	WP_044812807.1	zinc-dependent alcohol dehydrogenase family protein	-
R4709_RS06440	-	1260611..1260976 (+)	366	WP_000286415.1	DUF1033 family protein	-
R4709_RS06445	comGA/cglA/cilD	1261052..1261993 (+)	942	WP_000249567.1	competence type IV pilus ATPase ComGA	Machinery gene
R4709_RS06450	comGB/cglB	1261941..1262957 (+)	1017	WP_078132972.1	competence type IV pilus assembly protein ComGB	Machinery gene
R4709_RS06455	comGC/cglC	1262959..1263285 (+)	327	WP_000738625.1	competence type IV pilus major pilin ComGC	Machinery gene
R4709_RS06460	comGD/cglD	1263278..1263682 (+)	405	WP_000588030.1	competence type IV pilus minor pilin ComGD	Machinery gene
R4709_RS06465	comGE/cglE	1263645..1263947 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
R4709_RS06470	comGF/cglF	1263910..1264371 (+)	462	WP_000250539.1	competence type IV pilus minor pilin ComGF	Machinery gene
R4709_RS06475	comGG/cglG	1264349..1264795 (+)	447	WP_001811371.1	competence type IV pilus minor pilin ComGG	Machinery gene
R4709_RS06480	-	1264898..1265492 (+)	595	Protein_1266	class I SAM-dependent methyltransferase	-
R4709_RS06485	comYH	1265553..1266506 (+)	954	WP_044813830.1	class I SAM-dependent methyltransferase	Machinery gene
R4709_RS06490	-	1266557..1267747 (+)	1191	WP_050085692.1	acetate kinase	-
R4709_RS06495	-	1267748..1267879 (+)	132	WP_000768904.1	hypothetical protein	-
R4709_RS06500	rnpA	1267896..1268267 (+)	372	WP_000739246.1	ribonuclease P protein component	-
R4709_RS06505	-	1268242..1269066 (+)	825	WP_000727904.1	membrane protein insertase YidC	-

Sequence

Protein

Download Length: 153 a.a. Molecular weight: 17851.34 Da Isoelectric Point: 6.4832

                                >NTDB_id=895264 R4709_RS06470 WP_000250539.1 1263910..1264371(+) (comGF/cglF) [Streptococcus pneumoniae strain 16H3071-2]

MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN

RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

Download Length: 462 bp

                                >NTDB_id=895264 R4709_RS06470 WP_000250539.1 1263910..1264371(+) (comGF/cglF) [Streptococcus pneumoniae strain 16H3071-2]

ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT

TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG

AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT

CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG

TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT

TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae Rx1	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae D39	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae R6	94.118	100	0.941
comGF/cglF	Streptococcus pneumoniae TIGR4	94.118	100	0.941
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915

Detailed information NTG0895264