Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R4709_RS06450 Genome accession   NZ_CP137102
Coordinates   1261941..1262957 (+) Length   338 a.a.
NCBI ID   WP_078132972.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16H3071-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1256941..1267957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4709_RS06430 nagA 1258088..1259239 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4709_RS06435 - 1259402..1260460 (+) 1059 WP_044812807.1 zinc-dependent alcohol dehydrogenase family protein -
  R4709_RS06440 - 1260611..1260976 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4709_RS06445 comGA/cglA/cilD 1261052..1261993 (+) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  R4709_RS06450 comGB/cglB 1261941..1262957 (+) 1017 WP_078132972.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4709_RS06455 comGC/cglC 1262959..1263285 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  R4709_RS06460 comGD/cglD 1263278..1263682 (+) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4709_RS06465 comGE/cglE 1263645..1263947 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4709_RS06470 comGF/cglF 1263910..1264371 (+) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4709_RS06475 comGG/cglG 1264349..1264795 (+) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4709_RS06480 - 1264898..1265492 (+) 595 Protein_1266 class I SAM-dependent methyltransferase -
  R4709_RS06485 comYH 1265553..1266506 (+) 954 WP_044813830.1 class I SAM-dependent methyltransferase Machinery gene
  R4709_RS06490 - 1266557..1267747 (+) 1191 WP_050085692.1 acetate kinase -
  R4709_RS06495 - 1267748..1267879 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38406.42 Da        Isoelectric Point: 9.4802

>NTDB_id=895260 R4709_RS06450 WP_078132972.1 1261941..1262957(+) (comGB/cglB) [Streptococcus pneumoniae strain 16H3071-2]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=895260 R4709_RS06450 WP_078132972.1 1261941..1262957(+) (comGB/cglB) [Streptococcus pneumoniae strain 16H3071-2]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

99.704

100

0.997
  comGB/cglB Streptococcus pneumoniae D39

99.704

100

0.997
  comGB/cglB Streptococcus pneumoniae R6

99.704

100

0.997
  comGB/cglB Streptococcus pneumoniae TIGR4

99.704

100

0.997
  comGB/cglB Streptococcus mitis SK321

95.562

100

0.956
  comGB/cglB Streptococcus mitis NCTC 12261

94.97

100

0.95
  comYB Streptococcus gordonii str. Challis substr. CH1

71.429

99.408

0.71
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503