Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RY972_RS02690 Genome accession   NZ_CP136584
Coordinates   562144..563253 (+) Length   369 a.a.
NCBI ID   WP_163129014.1    Uniprot ID   -
Organism   Aeromonas allosaccharophila strain BIM B-1809     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 557144..568253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RY972_RS02660 (RY972_02660) - 558046..558465 (-) 420 WP_058053918.1 DUF4426 domain-containing protein -
  RY972_RS02665 (RY972_02665) yggU 558509..558811 (-) 303 WP_005341417.1 DUF167 family protein YggU -
  RY972_RS02670 (RY972_02670) - 558811..559362 (-) 552 WP_042011910.1 YggT family protein -
  RY972_RS02675 (RY972_02675) proC 559374..560198 (-) 825 WP_317103343.1 pyrroline-5-carboxylate reductase -
  RY972_RS02680 (RY972_02680) - 560340..561041 (-) 702 WP_075113900.1 YggS family pyridoxal phosphate-dependent enzyme -
  RY972_RS02685 (RY972_02685) pilT 561081..562115 (+) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  RY972_RS02690 (RY972_02690) pilU 562144..563253 (+) 1110 WP_163129014.1 type IVa pilus ATPase TapU Machinery gene
  RY972_RS02695 (RY972_02695) yaaA 563301..564074 (+) 774 WP_317103344.1 peroxide stress protein YaaA -
  RY972_RS02700 (RY972_02700) - 564269..564694 (+) 426 WP_317103345.1 hypothetical protein -
  RY972_RS02705 (RY972_02705) - 565056..565547 (-) 492 WP_317103346.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  RY972_RS02710 (RY972_02710) arfB 565573..565989 (-) 417 WP_075113904.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  RY972_RS02715 (RY972_02715) glnK 566191..566529 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  RY972_RS02720 (RY972_02720) - 566788..567795 (+) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41051.94 Da        Isoelectric Point: 6.0896

>NTDB_id=891735 RY972_RS02690 WP_163129014.1 562144..563253(+) (pilU) [Aeromonas allosaccharophila strain BIM B-1809]
MNLDELLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVIRRIETRIPTFEDLQLPNILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLQIKLSGQETLGSGTLENVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=891735 RY972_RS02690 WP_163129014.1 562144..563253(+) (pilU) [Aeromonas allosaccharophila strain BIM B-1809]
ATGAATCTGGATGAATTGCTGAGCGAACTGGTCGAACGAAAGGGCTCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
TCTCAAGGTCAATGGCCATCTGGTGTCGCTGGGGGGCGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGAGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCCATCTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGATACGTCGCATTGAGACCCGCATCCCCACCTTTGA
AGATCTGCAACTGCCCAACATCTTGCAGGAGGTCGCCATGGCCAAGCGGGGGTTGGTGCTGTTTGTCGGGGCCACCGGGG
CGGGTAAGTCCACTACTCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTCCATCAGCATGGCCGCAGTCTGGTGACCCAGCGTGAAGTGGGGATCGATACCGAGTCGTTCGATGT
GGCGCTGAAGAGCTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CTCTGCAGTTCGCCGAGACCGGTCACCTTTGCCTCGCCACTCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGGATC
CTCCATCTGGTGCCGCAAGACAAACATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGAAAGCCATCGTTGCCCAGCA
ACTGGTGCCAAGCATGGATGGCAAGCGGCGTTTTGCCGCGTTCGAGGTGCTGCTCAATACTCCGCTGATCACCGACATCA
TCCGCAAGGGTGAGATGCACCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGCATGCAGACCTTCGATCAGGCG
CTGTTCAGCCTGTTCTGTGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCTCATGCCGACTCGGCTAATGATTTGCGTCT
GCAAATCAAGCTCTCTGGTCAGGAGACGCTTGGCTCCGGCACGCTGGAGAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.141

99.729

0.61
  pilU Pseudomonas stutzeri DSM 10701

60.615

97.019

0.588
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.233

91.87

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36