Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RY972_RS02685 Genome accession   NZ_CP136584
Coordinates   561081..562115 (+) Length   344 a.a.
NCBI ID   WP_042063177.1    Uniprot ID   A0A6S5C6A2
Organism   Aeromonas allosaccharophila strain BIM B-1809     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 556081..567115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RY972_RS02655 (RY972_02655) - 556748..557944 (+) 1197 WP_042063182.1 NupC/NupG family nucleoside CNT transporter -
  RY972_RS02660 (RY972_02660) - 558046..558465 (-) 420 WP_058053918.1 DUF4426 domain-containing protein -
  RY972_RS02665 (RY972_02665) yggU 558509..558811 (-) 303 WP_005341417.1 DUF167 family protein YggU -
  RY972_RS02670 (RY972_02670) - 558811..559362 (-) 552 WP_042011910.1 YggT family protein -
  RY972_RS02675 (RY972_02675) proC 559374..560198 (-) 825 WP_317103343.1 pyrroline-5-carboxylate reductase -
  RY972_RS02680 (RY972_02680) - 560340..561041 (-) 702 WP_075113900.1 YggS family pyridoxal phosphate-dependent enzyme -
  RY972_RS02685 (RY972_02685) pilT 561081..562115 (+) 1035 WP_042063177.1 type IVa pilus ATPase TapT Machinery gene
  RY972_RS02690 (RY972_02690) pilU 562144..563253 (+) 1110 WP_163129014.1 type IVa pilus ATPase TapU Machinery gene
  RY972_RS02695 (RY972_02695) yaaA 563301..564074 (+) 774 WP_317103344.1 peroxide stress protein YaaA -
  RY972_RS02700 (RY972_02700) - 564269..564694 (+) 426 WP_317103345.1 hypothetical protein -
  RY972_RS02705 (RY972_02705) - 565056..565547 (-) 492 WP_317103346.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  RY972_RS02710 (RY972_02710) arfB 565573..565989 (-) 417 WP_075113904.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  RY972_RS02715 (RY972_02715) glnK 566191..566529 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38291.00 Da        Isoelectric Point: 6.7439

>NTDB_id=891734 RY972_RS02685 WP_042063177.1 561081..562115(+) (pilT) [Aeromonas allosaccharophila strain BIM B-1809]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKISEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=891734 RY972_RS02685 WP_042063177.1 561081..562115(+) (pilT) [Aeromonas allosaccharophila strain BIM B-1809]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGAGTCCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCTCGT
TTCCGGGTTAACGCCTTCCAGCAGGCGCGCGGCTCCGGCGCGGTATTTCGTACCATCCCCAGCACGGTCTTGAGCCTCGA
GGATCTCGATGCGCCTGAGATCTTTCGCAAGATCTCCGAATACCCGCGCGGTCTGGTGCTGGTGACCGGCCCGACCGGTT
CCGGTAAATCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATTCTCACCATCGAAGAC
CCTATCGAATTCGTTCATGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGTGACCTCGAGACCATTCGTCTGG
CCATGACCGCCGCCGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGTGCGGAGAAAGACATGGTGCGCTCCATGCTCTCCGAATCGCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGCATTGGTGGCGGCCGGGTGGCGGCACACGAGATCATGATGGGCATTCCGGCAGTGCGTAACCTGATCC
GTGAGGACAAGATCGCCCAGCTTTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5C6A2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.843

99.709

0.776
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter baumannii D1279779

77.551

99.709

0.773
  pilT Acinetobacter baumannii strain A118

77.551

99.709

0.773
  pilT Pseudomonas stutzeri DSM 10701

76.385

99.709

0.762
  pilT Pseudomonas aeruginosa PAK

75.802

99.709

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.353

98.837

0.715
  pilT Legionella pneumophila strain ERS1305867

72.353

98.837

0.715
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404