Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   WLF14_RS04355 Genome accession   NZ_CP151816
Coordinates   960742..961155 (+) Length   137 a.a.
NCBI ID   WP_024073385.1    Uniprot ID   A0ACA7P136
Organism   Pseudomonas fluorescens strain IMGN2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 955742..966155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WLF14_RS04320 (WLF14_04320) - 956210..956677 (+) 468 WP_069075203.1 FAD/FMN-containing dehydrogenase -
  WLF14_RS04325 (WLF14_04325) - 956674..957363 (+) 690 WP_024073381.1 energy-coupling factor ABC transporter permease -
  WLF14_RS04330 (WLF14_04330) - 957440..957655 (+) 216 WP_003171680.1 hypothetical protein -
  WLF14_RS04335 (WLF14_04335) yacG 957652..957852 (-) 201 WP_017845472.1 DNA gyrase inhibitor YacG -
  WLF14_RS04340 (WLF14_04340) coaE 957849..958472 (-) 624 WP_024073382.1 dephospho-CoA kinase -
  WLF14_RS04345 (WLF14_04345) pilD 958469..959335 (-) 867 WP_069075202.1 A24 family peptidase Machinery gene
  WLF14_RS04350 (WLF14_04350) pilC 959332..960540 (-) 1209 WP_069075201.1 type II secretion system F family protein Machinery gene
  WLF14_RS04355 (WLF14_04355) pilA/pilAI 960742..961155 (+) 414 WP_024073385.1 pilin Machinery gene
  WLF14_RS04360 (WLF14_04360) - 961190..962599 (+) 1410 WP_069075200.1 O-antigen ligase family protein -
  WLF14_RS04370 (WLF14_04370) - 962862..963167 (+) 306 WP_024073387.1 DUF6388 family protein -
  WLF14_RS04375 (WLF14_04375) - 963192..963281 (+) 90 Protein_851 outer membrane lipoprotein carrier protein LolA -
  WLF14_RS04380 (WLF14_04380) nadC 963289..964137 (-) 849 WP_150054127.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14030.26 Da        Isoelectric Point: 6.8994

>NTDB_id=890899 WLF14_RS04355 WP_024073385.1 960742..961155(+) (pilA/pilAI) [Pseudomonas fluorescens strain IMGN2]
MRQKGFTLIELLIVVAIIGILATIGLPMYTTHQAKAKFTAGLAEITALKAGYEDVFNQGTEPKLELVGGTSPTANCKIDV
AGDVATGNGSISCEILDAPAPVLGKTITLTRSATAGWKCVTTAEAKYAAKGCDASGA

Nucleotide


Download         Length: 414 bp        

>NTDB_id=890899 WLF14_RS04355 WP_024073385.1 960742..961155(+) (pilA/pilAI) [Pseudomonas fluorescens strain IMGN2]
ATGCGTCAGAAAGGCTTTACCTTGATCGAGTTGTTGATCGTCGTGGCAATCATCGGCATTTTGGCCACCATCGGCTTGCC
CATGTACACCACGCACCAGGCCAAGGCCAAGTTCACCGCGGGGCTGGCTGAGATCACCGCGTTGAAAGCCGGCTACGAGG
ATGTTTTCAACCAAGGCACCGAGCCGAAGCTGGAACTGGTCGGCGGCACCAGCCCGACCGCCAACTGCAAGATCGACGTG
GCCGGGGATGTCGCCACCGGTAACGGTTCCATCAGTTGCGAAATACTCGATGCCCCGGCGCCGGTGCTGGGCAAGACCAT
CACCCTGACGCGCAGCGCCACCGCCGGCTGGAAGTGCGTGACCACCGCTGAGGCGAAATACGCCGCCAAGGGTTGTGATG
CCAGCGGCGCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)

Pilin (bacterial filament) (Pilin)

(34-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.588

99.27

0.453
  pilA Acinetobacter baumannii strain A118

43.382

99.27

0.431
  pilA Pseudomonas aeruginosa PAK

39.31

100

0.416
  comP Acinetobacter baylyi ADP1

36.735

100

0.394
  pilA Vibrio cholerae strain A1552

38.028

100

0.394
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.028

100

0.394
  pilA Vibrio cholerae C6706

38.028

100

0.394
  pilA2 Legionella pneumophila strain ERS1305867

37.857

100

0.387
  pilA2 Legionella pneumophila str. Paris

37.857

100

0.387
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.778

98.54

0.372
  pilA Vibrio parahaemolyticus RIMD 2210633

36.232

100

0.365