Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   RRU92_RS03425 Genome accession   NZ_CP135436
Coordinates   670151..671092 (+) Length   313 a.a.
NCBI ID   WP_070676995.1    Uniprot ID   A0AA96MRP7
Organism   Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 665151..676092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRU92_RS03400 (RRU92_03400) - 665690..666499 (+) 810 WP_315640422.1 hypothetical protein -
  RRU92_RS03405 (RRU92_03405) - 666539..666736 (+) 198 WP_315640423.1 hypothetical protein -
  RRU92_RS03410 (RRU92_03410) nagA 667234..668385 (+) 1152 WP_315640424.1 N-acetylglucosamine-6-phosphate deacetylase -
  RRU92_RS03415 (RRU92_03415) - 668524..669582 (+) 1059 WP_315640425.1 zinc-dependent alcohol dehydrogenase family protein -
  RRU92_RS03420 (RRU92_03420) - 669720..670097 (+) 378 WP_315640427.1 DUF1033 family protein -
  RRU92_RS03425 (RRU92_03425) comGA/cglA/cilD 670151..671092 (+) 942 WP_070676995.1 competence type IV pilus ATPase ComGA Machinery gene
  RRU92_RS03430 (RRU92_03430) comGB/cglB 671013..672056 (+) 1044 WP_315640429.1 competence type IV pilus assembly protein ComGB Machinery gene
  RRU92_RS03435 (RRU92_03435) comGC/cglC 672057..672380 (+) 324 WP_315640431.1 competence type IV pilus major pilin ComGC Machinery gene
  RRU92_RS03440 (RRU92_03440) comGD/cglD 672343..672777 (+) 435 WP_075230215.1 competence type IV pilus minor pilin ComGD Machinery gene
  RRU92_RS03445 (RRU92_03445) comGE/cglE 672740..673042 (+) 303 WP_315640434.1 competence type IV pilus minor pilin ComGE Machinery gene
  RRU92_RS03450 (RRU92_03450) comGF/cglF 673005..673466 (+) 462 WP_315640436.1 competence type IV pilus minor pilin ComGF Machinery gene
  RRU92_RS03455 (RRU92_03455) comGG 673444..673881 (+) 438 WP_315640437.1 competence type IV pilus minor pilin ComGG -
  RRU92_RS03460 (RRU92_03460) - 673940..674641 (+) 702 WP_315640440.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35481.45 Da        Isoelectric Point: 6.0106

>NTDB_id=886525 RRU92_RS03425 WP_070676995.1 670151..671092(+) (comGA/cglA/cilD) [Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006]
MVQALAQELISLAKKDGAQDIYFIPKDKGYELHMRIGDERRLINSYDFEKLGAVISHFKFIAGMNVGEKRRSQLGSCDYQ
HDGKVSSLRLSTVGDYRGHESLVIRLLHDEEQELRFWFQDLKELEKGFRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEEMLQLQLNEAIGLSYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEDELAVVLQGVCYQRLIGGGGIIDFANQNYSEHQAEKWNEQIDQLLKDGHITALQAETEKISYQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=886525 RRU92_RS03425 WP_070676995.1 670151..671092(+) (comGA/cglA/cilD) [Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006]
ATGGTACAAGCACTTGCACAAGAATTGATTAGTCTCGCAAAAAAGGATGGAGCTCAGGATATCTATTTTATTCCCAAGGA
CAAGGGTTATGAGTTGCATATGCGAATAGGTGATGAAAGGCGTTTGATTAATAGCTATGACTTTGAAAAGTTGGGCGCAG
TGATTAGTCATTTTAAATTTATTGCGGGGATGAATGTTGGAGAAAAGCGTCGTAGTCAGCTTGGGTCCTGTGATTATCAG
CATGATGGCAAGGTTTCTTCACTCCGCTTATCGACGGTAGGAGATTATCGTGGTCATGAGAGTTTAGTTATTCGCCTTTT
GCACGATGAAGAACAAGAGTTGCGCTTCTGGTTTCAAGACCTTAAGGAACTAGAAAAGGGCTTTAGGCAACGCGGCCTTT
ATCTATTTGCAGGACCTGTAGGAAGTGGCAAAACTACCTTAATGCATGAATTAGCTAAGTCATTGTTTAAGGGACAACAA
GTCATGTCCATTGAGGATCCAGTCGAGATTAAGCAAGAAGAGATGCTGCAATTACAACTCAATGAAGCTATTGGATTGAG
TTATGAAAACTTGATAAAACTTTCTTTGCGACACCGACCAGATCTTTTAATCATTGGAGAAATCCGGGATAGCGAAACAG
CCCGTGCTGTTGTAAGAGCGAGCTTAACGGGAGCTACAGTGTTTTCGACCATACATGCAAAGAGTATTCGTGGGGTCTAT
GAGCGTCTTTTAGAACTTGGTGTTAGTGAAGATGAACTCGCAGTTGTTTTACAAGGTGTTTGCTACCAGAGACTAATCGG
GGGAGGAGGAATTATTGATTTTGCAAACCAAAATTATTCAGAACACCAGGCAGAAAAGTGGAATGAGCAAATTGATCAGC
TTCTTAAAGACGGACATATCACTGCCCTTCAAGCTGAAACGGAGAAAATTAGCTACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

86.218

99.681

0.859
  comGA/cglA/cilD Streptococcus pneumoniae D39

86.218

99.681

0.859
  comGA/cglA/cilD Streptococcus pneumoniae R6

86.218

99.681

0.859
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

86.218

99.681

0.859
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

85.897

99.681

0.856
  comYA Streptococcus gordonii str. Challis substr. CH1

77.636

100

0.776
  comYA Streptococcus mutans UA159

65.273

99.361

0.649
  comYA Streptococcus mutans UA140

65.273

99.361

0.649
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

63.226

99.042

0.626
  comGA Lactococcus lactis subsp. cremoris KW2

57.051

99.681

0.569
  comGA Latilactobacillus sakei subsp. sakei 23K

43.841

88.179

0.387