Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   RRU92_RS03450 Genome accession   NZ_CP135436
Coordinates   673005..673466 (+) Length   153 a.a.
NCBI ID   WP_315640436.1    Uniprot ID   A0AA96S3Q1
Organism   Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 668005..678466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRU92_RS03415 (RRU92_03415) - 668524..669582 (+) 1059 WP_315640425.1 zinc-dependent alcohol dehydrogenase family protein -
  RRU92_RS03420 (RRU92_03420) - 669720..670097 (+) 378 WP_315640427.1 DUF1033 family protein -
  RRU92_RS03425 (RRU92_03425) comGA/cglA/cilD 670151..671092 (+) 942 WP_070676995.1 competence type IV pilus ATPase ComGA Machinery gene
  RRU92_RS03430 (RRU92_03430) comGB/cglB 671013..672056 (+) 1044 WP_315640429.1 competence type IV pilus assembly protein ComGB Machinery gene
  RRU92_RS03435 (RRU92_03435) comGC/cglC 672057..672380 (+) 324 WP_315640431.1 competence type IV pilus major pilin ComGC Machinery gene
  RRU92_RS03440 (RRU92_03440) comGD/cglD 672343..672777 (+) 435 WP_075230215.1 competence type IV pilus minor pilin ComGD Machinery gene
  RRU92_RS03445 (RRU92_03445) comGE/cglE 672740..673042 (+) 303 WP_315640434.1 competence type IV pilus minor pilin ComGE Machinery gene
  RRU92_RS03450 (RRU92_03450) comGF/cglF 673005..673466 (+) 462 WP_315640436.1 competence type IV pilus minor pilin ComGF Machinery gene
  RRU92_RS03455 (RRU92_03455) comGG 673444..673881 (+) 438 WP_315640437.1 competence type IV pilus minor pilin ComGG -
  RRU92_RS03460 (RRU92_03460) - 673940..674641 (+) 702 WP_315640440.1 histidine phosphatase family protein -
  RRU92_RS03465 (RRU92_03465) - 674831..676537 (+) 1707 WP_315640441.1 ABC transporter ATP-binding protein -
  RRU92_RS03470 (RRU92_03470) - 676539..678305 (+) 1767 WP_311522999.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 18254.92 Da        Isoelectric Point: 10.1547

>NTDB_id=886530 RRU92_RS03450 WP_315640436.1 673005..673466(+) (comGF/cglF) [Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006]
MERRNYWRFKISRVRAFTLLESLVALIVISGSLLLFQSMSRLLVSEVRYQQRSEQKEWLLFVDQLEAELNRAQFEKVEND
RLYLKQDGKPISMGKSRSDDFRKTDASGRGYQPMVYGLKSAHIYQKGQNVHFNFQFEKGLEREFIYRVEKEKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=886530 RRU92_RS03450 WP_315640436.1 673005..673466(+) (comGF/cglF) [Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006]
ATGGAACGGAGAAATTACTGGAGATTCAAGATAAGTAGAGTCAGAGCGTTTACTCTTTTAGAATCCTTGGTTGCCCTTAT
CGTTATTAGTGGTAGCTTGCTTCTTTTCCAATCTATGAGCCGACTCTTGGTTTCAGAGGTTCGCTATCAGCAGAGAAGCG
AGCAGAAGGAGTGGCTCTTATTTGTGGATCAACTAGAAGCGGAGTTGAATCGAGCCCAGTTTGAAAAAGTTGAGAATGAT
AGACTTTATCTCAAACAAGATGGTAAACCTATTTCCATGGGTAAATCAAGGTCAGATGATTTTCGGAAAACAGATGCCAG
TGGACGTGGCTATCAACCCATGGTTTATGGCCTTAAATCAGCACATATTTACCAAAAAGGGCAAAATGTTCATTTCAATT
TTCAGTTTGAAAAGGGACTAGAGAGGGAGTTTATCTATCGTGTTGAAAAAGAAAAAAGTTAG

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(46-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis NCTC 12261

76.471

100

0.765
  comGF/cglF Streptococcus mitis SK321

75.817

100

0.758
  comGF/cglF Streptococcus pneumoniae Rx1

74.51

100

0.745
  comGF/cglF Streptococcus pneumoniae D39

74.51

100

0.745
  comGF/cglF Streptococcus pneumoniae R6

74.51

100

0.745
  comGF/cglF Streptococcus pneumoniae TIGR4

74.51

100

0.745
  comGF Lactococcus lactis subsp. cremoris KW2

40.714

91.503

0.373