Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   RRU92_RS03430 Genome accession   NZ_CP135436
Coordinates   671013..672056 (+) Length   347 a.a.
NCBI ID   WP_315640429.1    Uniprot ID   A0AA96MR20
Organism   Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 666013..677056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRU92_RS03405 (RRU92_03405) - 666539..666736 (+) 198 WP_315640423.1 hypothetical protein -
  RRU92_RS03410 (RRU92_03410) nagA 667234..668385 (+) 1152 WP_315640424.1 N-acetylglucosamine-6-phosphate deacetylase -
  RRU92_RS03415 (RRU92_03415) - 668524..669582 (+) 1059 WP_315640425.1 zinc-dependent alcohol dehydrogenase family protein -
  RRU92_RS03420 (RRU92_03420) - 669720..670097 (+) 378 WP_315640427.1 DUF1033 family protein -
  RRU92_RS03425 (RRU92_03425) comGA/cglA/cilD 670151..671092 (+) 942 WP_070676995.1 competence type IV pilus ATPase ComGA Machinery gene
  RRU92_RS03430 (RRU92_03430) comGB/cglB 671013..672056 (+) 1044 WP_315640429.1 competence type IV pilus assembly protein ComGB Machinery gene
  RRU92_RS03435 (RRU92_03435) comGC/cglC 672057..672380 (+) 324 WP_315640431.1 competence type IV pilus major pilin ComGC Machinery gene
  RRU92_RS03440 (RRU92_03440) comGD/cglD 672343..672777 (+) 435 WP_075230215.1 competence type IV pilus minor pilin ComGD Machinery gene
  RRU92_RS03445 (RRU92_03445) comGE/cglE 672740..673042 (+) 303 WP_315640434.1 competence type IV pilus minor pilin ComGE Machinery gene
  RRU92_RS03450 (RRU92_03450) comGF/cglF 673005..673466 (+) 462 WP_315640436.1 competence type IV pilus minor pilin ComGF Machinery gene
  RRU92_RS03455 (RRU92_03455) comGG 673444..673881 (+) 438 WP_315640437.1 competence type IV pilus minor pilin ComGG -
  RRU92_RS03460 (RRU92_03460) - 673940..674641 (+) 702 WP_315640440.1 histidine phosphatase family protein -
  RRU92_RS03465 (RRU92_03465) - 674831..676537 (+) 1707 WP_315640441.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39726.16 Da        Isoelectric Point: 9.7830

>NTDB_id=886526 RRU92_RS03430 WP_315640429.1 671013..672056(+) (comGB/cglB) [Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006]
MSKLISFLKTDISLPFKLKRRKLATSKQKKIITLFNNLFSSGFHLVEIISFLDRSLLLEKEYVSQMRLGLSKGKSFSEMM
GNLGFSSAIVTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLAFLLLIMLGLRNYLLPQLDSSNIATL
VISNLPQIFLGLILALSLASLTGLIFYRKSKKIQVFSYLARIPFLGVFVQYYLTAYYAREWGNMIGQGLELAQIFQMMQE
QGNLLFREIGHDLEQTLQSGQEFSKVVQDYPFFRKELGLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPMV
FIFVALLIVLLYAAMLLPMYQNMEVNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=886526 RRU92_RS03430 WP_315640429.1 671013..672056(+) (comGB/cglB) [Streptococcus sp. DTU_2020_1001019_1_SI_AUS_MUR_006]
ATGAGCAAATTGATCAGCTTCTTAAAGACGGACATATCACTGCCCTTCAAGCTGAAACGGAGAAAATTAGCTACCAGTAA
GCAGAAGAAAATCATCACTCTCTTTAACAATTTGTTTTCTAGTGGTTTTCACTTGGTAGAAATCATTTCCTTTCTTGATA
GAAGTCTTTTGTTGGAGAAGGAATATGTTTCGCAGATGCGCCTAGGACTATCGAAAGGAAAATCTTTCTCGGAAATGATG
GGGAATTTAGGTTTTTCGAGTGCGATTGTCACTCAGCTTTCATTGGCTGAAGTTCATGGAAATCTCCATTTGAGTTTGGG
GAAAATTGAAGAATATTTGGATAATCTTGCTAAGGTCAAGAAGAAGTTAATTGAAGTTGCGACCTACCCACTTATTTTGC
TAGCTTTTTTACTGTTAATTATGCTAGGATTGCGGAATTATCTCTTGCCCCAACTGGATAGTAGTAACATAGCTACCTTA
GTGATTAGTAACCTGCCACAGATTTTTCTCGGACTTATTCTGGCACTAAGTCTTGCATCTTTAACAGGACTGATATTCTA
CAGAAAATCTAAGAAGATACAAGTCTTTTCTTATCTGGCTAGAATCCCCTTTTTAGGTGTGTTTGTTCAGTACTATCTAA
CTGCTTATTATGCTCGGGAATGGGGAAATATGATTGGACAAGGTTTAGAGTTGGCACAGATTTTCCAGATGATGCAGGAG
CAAGGGAATCTTCTTTTTAGGGAAATAGGACACGATTTAGAGCAGACCCTACAAAGTGGGCAGGAATTTTCTAAAGTTGT
GCAAGATTATCCTTTCTTTAGAAAGGAGTTGGGATTAATCATTGAATATGGAGAGGTCAAGTCTAAGTTGGGAAGCGAAT
TGGAAATCTATGCGGAAAAAACTTGGGAGGCATTCTTTACCAGAGTCAATCGTACTATGAATCTAGTACAACCTATGGTT
TTTATTTTTGTGGCCCTACTAATCGTTTTATTGTACGCAGCTATGTTGCTGCCCATGTATCAAAATATGGAGGTAAATTT
TTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

81.602

97.118

0.793
  comGB/cglB Streptococcus mitis NCTC 12261

81.306

97.118

0.79
  comGB/cglB Streptococcus pneumoniae Rx1

81.009

97.118

0.787
  comGB/cglB Streptococcus pneumoniae D39

81.009

97.118

0.787
  comGB/cglB Streptococcus pneumoniae R6

81.009

97.118

0.787
  comGB/cglB Streptococcus pneumoniae TIGR4

81.009

97.118

0.787
  comYB Streptococcus gordonii str. Challis substr. CH1

71.304

99.424

0.709
  comYB Streptococcus mutans UA140

58.892

98.847

0.582
  comYB Streptococcus mutans UA159

58.892

98.847

0.582
  comGB Lactococcus lactis subsp. cremoris KW2

49.852

97.118

0.484