Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   RHP74_RS26585 Genome accession   NZ_CP134498
Coordinates   5788385..5788792 (-) Length   135 a.a.
NCBI ID   WP_311096685.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 5783385..5793792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP74_RS26570 (RHP74_26570) - 5784183..5787137 (+) 2955 WP_311096684.1 DUF748 domain-containing protein -
  RHP74_RS26575 (RHP74_26575) - 5787137..5787433 (+) 297 WP_154501766.1 DUF2845 domain-containing protein -
  RHP74_RS26580 (RHP74_26580) - 5787719..5788072 (-) 354 WP_010467586.1 BON domain-containing protein -
  RHP74_RS26585 (RHP74_26585) pilA 5788385..5788792 (-) 408 WP_311096685.1 pilin Machinery gene
  RHP74_RS26590 (RHP74_26590) pilB 5789018..5790718 (+) 1701 WP_010467584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHP74_RS26595 (RHP74_26595) pilC 5790721..5791938 (+) 1218 WP_010467583.1 type II secretion system F family protein Machinery gene
  RHP74_RS26600 (RHP74_26600) pilD 5791940..5792812 (+) 873 WP_311096686.1 A24 family peptidase Machinery gene
  RHP74_RS26605 (RHP74_26605) coaE 5792809..5793432 (+) 624 WP_042932554.1 dephospho-CoA kinase -
  RHP74_RS26610 (RHP74_26610) yacG 5793429..5793629 (+) 201 WP_154913029.1 DNA gyrase inhibitor YacG -

Sequence


Protein


Download         Length: 135 a.a.        Molecular weight: 13814.95 Da        Isoelectric Point: 7.5892

>NTDB_id=880543 RHP74_RS26585 WP_311096685.1 5788385..5788792(-) (pilA) [Pseudomonas sp. SG20052]
MNTQKGFTLIELLIVVAIIGILATFALPAYSKYQARAKVTAGLAEITALKVPFEDLMNSGTNPDLTKVGGTATTSNCTTT
AAGVATDGTGTIGCTILNAPGPVLSKTITLTRSLTGWTCATTVDQEYAPKGCTGV

Nucleotide


Download         Length: 408 bp        

>NTDB_id=880543 RHP74_RS26585 WP_311096685.1 5788385..5788792(-) (pilA) [Pseudomonas sp. SG20052]
ATGAATACTCAAAAAGGTTTTACCCTGATCGAACTGCTGATCGTGGTGGCGATCATCGGGATTCTGGCGACGTTTGCGTT
GCCGGCTTATTCGAAGTATCAAGCGCGTGCCAAGGTGACGGCCGGTCTGGCAGAGATCACGGCGTTGAAAGTCCCGTTTG
AGGACCTCATGAACTCTGGTACCAATCCGGATCTGACAAAAGTGGGCGGAACCGCCACGACTTCCAATTGCACAACCACG
GCAGCGGGTGTCGCGACTGATGGTACCGGCACCATCGGTTGCACGATCCTCAACGCTCCAGGTCCGGTCTTGAGCAAAAC
CATTACCCTGACGCGCTCTCTGACTGGCTGGACTTGCGCCACCACAGTGGATCAGGAGTACGCACCTAAAGGCTGCACAG
GCGTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50

100

0.511

  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.175

100

0.489

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.958

100

0.452

  pilA Vibrio cholerae strain A1552

42.958

100

0.452

  pilA Vibrio cholerae C6706

42.958

100

0.452

  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

100

0.444

  pilA Pseudomonas aeruginosa PAK

40.268

100

0.444

  comP Acinetobacter baylyi ADP1

36.054

100

0.393

  pilA2 Legionella pneumophila str. Paris

37.778

100

0.378

  pilA2 Legionella pneumophila strain ERS1305867

37.037

100

0.37