Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHP74_RS26590 Genome accession   NZ_CP134498
Coordinates   5789018..5790718 (+) Length   566 a.a.
NCBI ID   WP_010467584.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20052     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5784018..5795718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP74_RS26570 (RHP74_26570) - 5784183..5787137 (+) 2955 WP_311096684.1 DUF748 domain-containing protein -
  RHP74_RS26575 (RHP74_26575) - 5787137..5787433 (+) 297 WP_154501766.1 DUF2845 domain-containing protein -
  RHP74_RS26580 (RHP74_26580) - 5787719..5788072 (-) 354 WP_010467586.1 BON domain-containing protein -
  RHP74_RS26585 (RHP74_26585) pilA 5788385..5788792 (-) 408 WP_311096685.1 pilin Machinery gene
  RHP74_RS26590 (RHP74_26590) pilB 5789018..5790718 (+) 1701 WP_010467584.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHP74_RS26595 (RHP74_26595) pilC 5790721..5791938 (+) 1218 WP_010467583.1 type II secretion system F family protein Machinery gene
  RHP74_RS26600 (RHP74_26600) pilD 5791940..5792812 (+) 873 WP_311096686.1 A24 family peptidase Machinery gene
  RHP74_RS26605 (RHP74_26605) coaE 5792809..5793432 (+) 624 WP_042932554.1 dephospho-CoA kinase -
  RHP74_RS26610 (RHP74_26610) yacG 5793429..5793629 (+) 201 WP_154913029.1 DNA gyrase inhibitor YacG -
  RHP74_RS26615 (RHP74_26615) - 5793633..5793848 (-) 216 WP_010467577.1 hypothetical protein -
  RHP74_RS26620 (RHP74_26620) - 5793912..5794601 (-) 690 WP_083375730.1 energy-coupling factor ABC transporter permease -
  RHP74_RS26625 (RHP74_26625) - 5794598..5795065 (-) 468 WP_010467572.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62669.93 Da        Isoelectric Point: 5.8860

>NTDB_id=880544 RHP74_RS26590 WP_010467584.1 5789018..5790718(+) (pilB) [Pseudomonas sp. SG20052]
MNDIALSGLAKQLVMAELLTEQIAQQAYLQAQRSKISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLNCLDKEAQPRGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLGDAIEKFFDNQATGLEDMADVDLDG
LDIESVDDNKKDAIAGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPSQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKALYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLHNMGIQGFNIATSVTLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDQCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLIKAMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=880544 RHP74_RS26590 WP_010467584.1 5789018..5790718(+) (pilB) [Pseudomonas sp. SG20052]
ATGAATGACATCGCCCTAAGCGGTCTGGCCAAGCAACTGGTCATGGCCGAACTGCTCACTGAGCAAATCGCGCAACAGGC
GTATCTGCAAGCCCAACGCAGCAAAATCTCCCTGGTCAGTTACCTGGTGCAGAACAAGCTGGTGAAGAGCCGGCAGGTTG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCGCTGCTCGACCTCAATTGCCTGGACAAGGAAGCCCAGCCTCGAGGGTTG
GTCAGCGAGAAACTGATCCGTCAGCACCACGCCCTGCCCCTCTGGCGCCGTGGCAACAAACTGTTCGTGGGGGTTTCCGA
CCCGACCAATCATCAAGCCATCAACGACATCCAGTTCAGCACCGGTCTGAACACCGAAGCCATTCTGGTAGAGGACGATA
AGCTCGGCGATGCCATCGAGAAGTTCTTCGACAACCAAGCAACCGGCCTGGAAGACATGGCCGATGTTGACCTTGACGGG
CTGGACATCGAGTCGGTCGACGACAACAAAAAAGACGCCATCGCCGGCCAGGATGCCGATGACGCGCCGGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGGG
TGCGGATGCGCACCGACGGCATGCTGCGAGAAGTCGCCAAGCCGCCGAGCCAACTGGCCAACCGCATTGCGGCGCGGCTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGACGGATCAAGATGCGCCTGTCCAAGACCAA
GTCCATCGACTTCCGGGTCAACACCTTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGCCTGAACAGAAAGCCTTGTACATGGCTGCACTGAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGGCCGACCGGTTCGGGCAAGACCGTGTCGCTCTACACCGGGTTGAATATCCTCAACACCGTGGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCAAGCAAG
GGCTGGATTTCGCCCAGGCTCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGTGAGATCCGCGACCTC
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGCCTGCACAACATGGGCATTCAGGGCTTCAACATCGCCACCTCGGTCACCCTGATCATCGCCCAGCGGC
TGGCGCGCAAACTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGGCGTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGCGATCAATGCAACGGCGGTTACAAGGGGCGAGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCCCTGGAAATCGACATTCAGA
TGCGCAAGGACGGTTTTAATGACTTGCGCACCTCGGGCTTGATCAAGGCCATGCAAGGCGTCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.47

0.588
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Legionella pneumophila strain ERS1305867

55.398

99.823

0.553
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.376

99.117

0.489
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.463

0.366