Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   RIM63_RS00710 Genome accession   NZ_CP133952
Coordinates   125561..126499 (+) Length   312 a.a.
NCBI ID   WP_206158897.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain HT321     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 120561..131499
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIM63_RS00695 (RIM63_00695) - 120923..122155 (+) 1233 WP_014622136.1 hypothetical protein -
  RIM63_RS00700 (RIM63_00700) - 122728..124731 (+) 2004 WP_317584300.1 DUF1430 domain-containing protein -
  RIM63_RS00705 (RIM63_00705) - 124733..125380 (+) 648 WP_317584302.1 ABC transporter ATP-binding protein -
  RIM63_RS00710 (RIM63_00710) comGA/cglA 125561..126499 (+) 939 WP_206158897.1 competence type IV pilus ATPase ComGA Machinery gene
  RIM63_RS00715 (RIM63_00715) comYB 126432..127466 (+) 1035 WP_249093266.1 competence type IV pilus assembly protein ComGB Machinery gene
  RIM63_RS00720 (RIM63_00720) comGC/cglC 127467..127790 (+) 324 WP_014622142.1 competence type IV pilus major pilin ComGC Machinery gene
  RIM63_RS00725 (RIM63_00725) comGD 127768..128193 (+) 426 WP_317585222.1 competence type IV pilus minor pilin ComGD -
  RIM63_RS00730 (RIM63_00730) comGE 128198..128440 (+) 243 WP_012677236.1 competence type IV pilus minor pilin ComGE -
  RIM63_RS00735 (RIM63_00735) comYF 128427..128861 (+) 435 WP_012677237.1 competence type IV pilus minor pilin ComGF Machinery gene
  RIM63_RS00740 (RIM63_00740) comGG 128839..129201 (+) 363 WP_206152302.1 competence type IV pilus minor pilin ComGG -
  RIM63_RS00745 (RIM63_00745) comYH 129263..130216 (+) 954 WP_037580627.1 class I SAM-dependent methyltransferase Machinery gene
  RIM63_RS00750 (RIM63_00750) - 130276..131475 (+) 1200 WP_012678817.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35382.60 Da        Isoelectric Point: 7.1375

>NTDB_id=877740 RIM63_RS00710 WP_206158897.1 125561..126499(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain HT321]
MIQELAKRLIRQAEELQAQDIYILPRGTGYELLMRVGDDRRLIDVCESDRMANLISHFKFVAGMNVGEKRRCQLGACDYD
LDDTKVSLRLSAVGDYQGQESLVIRLLYHHQRQLRYWFNGLERVTSAIGARGLYLFSGPVGSGKTTLMYQLVSDYCKELQ
VISIEDPVEIKNDQLLQLQVNDSIGMTYDNLIKLSLRHRPDVLIIGEIRDTQTARAVIRASLTGAMVFSTVHAKSISGVY
ARLLELGISRAELDNSLAMVAYQRFISGGALIDCAQKEFEHHQANRWNQQIDQLLAEGHLNSRQARLEKIIQ

Nucleotide


Download         Length: 939 bp        

>NTDB_id=877740 RIM63_RS00710 WP_206158897.1 125561..126499(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain HT321]
ATGATTCAAGAATTAGCCAAGCGGCTTATCAGGCAGGCTGAAGAATTGCAGGCTCAGGATATTTATATACTACCAAGGGG
GACAGGCTATGAGCTTTTGATGAGGGTAGGTGATGACAGGAGGCTAATAGATGTTTGTGAAAGTGATCGGATGGCTAATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGAATGTGGGAGAAAAAAGACGCTGTCAGCTCGGTGCTTGTGACTACGAC
CTTGATGACACTAAGGTGTCTCTACGATTATCAGCTGTGGGTGATTATCAGGGACAGGAGAGCTTAGTTATTCGTCTCTT
GTATCATCATCAACGCCAGCTCAGGTATTGGTTTAATGGTTTAGAGCGAGTCACATCAGCTATTGGAGCTAGAGGCCTTT
ACCTCTTTTCTGGACCAGTCGGATCAGGTAAGACAACCCTGATGTATCAGCTAGTCTCTGACTATTGCAAGGAGCTTCAG
GTGATCAGTATTGAGGATCCTGTGGAAATAAAAAATGACCAGCTCCTGCAATTACAGGTTAACGATAGCATTGGCATGAC
ATACGATAATCTGATTAAGCTTTCCTTGCGTCATCGTCCAGATGTGTTGATTATCGGAGAAATTAGAGATACACAGACTG
CTAGAGCGGTTATTAGGGCTAGCCTAACAGGAGCAATGGTCTTTTCAACCGTCCATGCAAAGAGTATTTCAGGCGTTTAC
GCTAGGCTGCTAGAATTAGGCATTTCTAGGGCAGAATTGGATAATAGTCTAGCAATGGTTGCTTACCAGCGATTTATTAG
TGGAGGTGCTTTAATTGATTGTGCGCAAAAGGAATTTGAACATCATCAAGCCAACCGGTGGAATCAGCAGATTGATCAGC
TTCTTGCAGAGGGACATCTCAATAGCAGGCAGGCAAGGCTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

67.419

99.359

0.67
  comYA Streptococcus mutans UA159

63.344

99.679

0.631
  comYA Streptococcus mutans UA140

63.344

99.679

0.631
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

61.935

99.359

0.615
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.613

99.359

0.612
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.613

99.359

0.612
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.613

99.359

0.612
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.613

99.359

0.612
  comYA Streptococcus gordonii str. Challis substr. CH1

61.29

99.359

0.609
  comGA Lactococcus lactis subsp. cremoris KW2

52.09

99.679

0.519
  comGA Latilactobacillus sakei subsp. sakei 23K

40.909

91.667

0.375