Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   RIM63_RS00735 Genome accession   NZ_CP133952
Coordinates   128427..128861 (+) Length   144 a.a.
NCBI ID   WP_012677237.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain HT321     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 123427..133861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIM63_RS00705 (RIM63_00705) - 124733..125380 (+) 648 WP_317584302.1 ABC transporter ATP-binding protein -
  RIM63_RS00710 (RIM63_00710) comGA/cglA 125561..126499 (+) 939 WP_206158897.1 competence type IV pilus ATPase ComGA Machinery gene
  RIM63_RS00715 (RIM63_00715) comYB 126432..127466 (+) 1035 WP_249093266.1 competence type IV pilus assembly protein ComGB Machinery gene
  RIM63_RS00720 (RIM63_00720) comGC/cglC 127467..127790 (+) 324 WP_014622142.1 competence type IV pilus major pilin ComGC Machinery gene
  RIM63_RS00725 (RIM63_00725) comGD 127768..128193 (+) 426 WP_317585222.1 competence type IV pilus minor pilin ComGD -
  RIM63_RS00730 (RIM63_00730) comGE 128198..128440 (+) 243 WP_012677236.1 competence type IV pilus minor pilin ComGE -
  RIM63_RS00735 (RIM63_00735) comYF 128427..128861 (+) 435 WP_012677237.1 competence type IV pilus minor pilin ComGF Machinery gene
  RIM63_RS00740 (RIM63_00740) comGG 128839..129201 (+) 363 WP_206152302.1 competence type IV pilus minor pilin ComGG -
  RIM63_RS00745 (RIM63_00745) comYH 129263..130216 (+) 954 WP_037580627.1 class I SAM-dependent methyltransferase Machinery gene
  RIM63_RS00750 (RIM63_00750) - 130276..131475 (+) 1200 WP_012678817.1 acetate kinase -
  RIM63_RS00755 (RIM63_00755) - 131844..132458 (-) 615 WP_317584310.1 hypothetical protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16155.70 Da        Isoelectric Point: 10.1988

>NTDB_id=877743 RIM63_RS00735 WP_012677237.1 128427..128861(+) (comYF) [Streptococcus equi subsp. zooepidemicus strain HT321]
MKDSRLKAFTLIECLIALLVISGSLLVYQALTKSLMVSERYLAANDQDNWLLFSQQLRAELSGTTLQGVSNNRLYVEKDK
KTLSFGQVKSHDFRKAAGNGRGYQPMLFGLSSSQITAVGQQVIIKLKWQSGLERTFIYAFQEKG

Nucleotide


Download         Length: 435 bp        

>NTDB_id=877743 RIM63_RS00735 WP_012677237.1 128427..128861(+) (comYF) [Streptococcus equi subsp. zooepidemicus strain HT321]
TTGAAAGACAGTAGGTTAAAGGCTTTCACCTTGATAGAGTGCCTTATTGCCTTGCTTGTCATCTCAGGCTCTTTATTAGT
TTATCAGGCCTTAACCAAGAGCCTTATGGTGAGTGAGAGGTATCTAGCAGCAAATGATCAGGACAACTGGCTTTTGTTTT
CCCAGCAATTGCGAGCAGAGCTTTCAGGTACTACCTTACAGGGTGTCTCCAATAATAGGCTATATGTTGAGAAAGACAAG
AAAACTCTGTCCTTTGGACAGGTCAAAAGCCATGATTTTAGAAAAGCAGCTGGCAATGGTCGAGGCTATCAGCCCATGCT
GTTTGGCTTGTCAAGTAGCCAAATAACAGCAGTAGGTCAGCAGGTTATCATCAAGCTGAAATGGCAAAGCGGCTTAGAAA
GGACCTTTATTTATGCATTTCAAGAGAAGGGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

52.083

100

0.521
  comYF Streptococcus mutans UA159

51.389

100

0.514
  comGF/cglF Streptococcus mitis SK321

50.365

95.139

0.479
  comGF/cglF Streptococcus mitis NCTC 12261

49.635

95.139

0.472
  comGF Lactococcus lactis subsp. cremoris KW2

47.143

97.222

0.458
  comGF/cglF Streptococcus pneumoniae D39

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae R6

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae TIGR4

48.175

95.139

0.458
  comGF/cglF Streptococcus pneumoniae Rx1

48.175

95.139

0.458