Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RF668_RS04155 Genome accession   NZ_CP133787
Coordinates   823949..824929 (+) Length   326 a.a.
NCBI ID   WP_043736167.1    Uniprot ID   A0AAX4A994
Organism   Lactococcus cremoris strain KCKM 0438     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 818949..829929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RF668_RS04145 (RF668_04145) - 818966..819472 (+) 507 Protein_766 PolC-type DNA polymerase III N-terminal domain-containing protein -
  RF668_RS04150 (RF668_04150) - 820061..823834 (+) 3774 Protein_767 PolC-type DNA polymerase III -
  RF668_RS04155 (RF668_04155) comGA 823949..824929 (+) 981 WP_043736167.1 competence type IV pilus ATPase ComGA Machinery gene
  RF668_RS04160 (RF668_04160) comGB 824829..825854 (+) 1026 WP_052206589.1 competence type IV pilus assembly protein ComGB Machinery gene
  RF668_RS04165 (RF668_04165) comGC 825898..826197 (+) 300 WP_052206590.1 competence type IV pilus major pilin ComGC Machinery gene
  RF668_RS04170 (RF668_04170) comGD 826208..826639 (+) 432 WP_050750513.1 competence type IV pilus minor pilin ComGD Machinery gene
  RF668_RS04175 (RF668_04175) comGE 826611..826907 (+) 297 WP_043736169.1 competence type IV pilus minor pilin ComGE Machinery gene
  RF668_RS04180 (RF668_04180) comGF 826891..827316 (+) 426 WP_374049086.1 competence type IV pilus minor pilin ComGF Machinery gene
  RF668_RS04185 (RF668_04185) comGG 827340..827639 (+) 300 WP_021037947.1 competence type IV pilus minor pilin ComGG Machinery gene
  RF668_RS04190 (RF668_04190) - 827720..828157 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  RF668_RS04195 (RF668_04195) - 828154..828996 (+) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  RF668_RS04200 (RF668_04200) - 829175..829912 (+) 738 WP_011836039.1 metal ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 37019.37 Da        Isoelectric Point: 6.1811

>NTDB_id=876866 RF668_RS04155 WP_043736167.1 823949..824929(+) (comGA) [Lactococcus cremoris strain KCKM 0438]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFAGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIDSDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIEKLYANGHLTTIEAEREKISINQASKIDTT
DGESFE

Nucleotide


Download         Length: 981 bp        

>NTDB_id=876866 RF668_RS04155 WP_043736167.1 823949..824929(+) (comGA) [Lactococcus cremoris strain KCKM 0438]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGGTTGGCTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAATTAGGGGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAACGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGATGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTGCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGATTCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACAGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTAAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCCAAAAAATGGAATGAAAAGATTGAAAAATTAT
ATGCAAACGGACATCTTACGACTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

98.773

100

0.988
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

56.129

95.092

0.534
  comGA/cglA/cilD Streptococcus pneumoniae D39

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae R6

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.808

95.706

0.525
  comYA Streptococcus gordonii str. Challis substr. CH1

54.114

96.933

0.525
  comYA Streptococcus mutans UA140

52.412

95.399

0.5
  comYA Streptococcus mutans UA159

52.412

95.399

0.5