Detailed information NTG0876871

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: RF668_RS04180
• Genome accession: NZ_CP133787
• Coordinates: 826891..827316 (+)
• Length: 141 a.a.
• NCBI ID: WP_374049086.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain KCKM 0438
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 821891..832316

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RF668_RS04155 (RF668_04155)	comGA	823949..824929 (+)	981	WP_043736167.1	competence type IV pilus ATPase ComGA	Machinery gene
RF668_RS04160 (RF668_04160)	comGB	824829..825854 (+)	1026	WP_052206589.1	competence type IV pilus assembly protein ComGB	Machinery gene
RF668_RS04165 (RF668_04165)	comGC	825898..826197 (+)	300	WP_052206590.1	competence type IV pilus major pilin ComGC	Machinery gene
RF668_RS04170 (RF668_04170)	comGD	826208..826639 (+)	432	WP_050750513.1	competence type IV pilus minor pilin ComGD	Machinery gene
RF668_RS04175 (RF668_04175)	comGE	826611..826907 (+)	297	WP_043736169.1	competence type IV pilus minor pilin ComGE	Machinery gene
RF668_RS04180 (RF668_04180)	comGF	826891..827316 (+)	426	WP_374049086.1	competence type IV pilus minor pilin ComGF	Machinery gene
RF668_RS04185 (RF668_04185)	comGG	827340..827639 (+)	300	WP_021037947.1	competence type IV pilus minor pilin ComGG	Machinery gene
RF668_RS04190 (RF668_04190)	-	827720..828157 (+)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
RF668_RS04195 (RF668_04195)	-	828154..828996 (+)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
RF668_RS04200 (RF668_04200)	-	829175..829912 (+)	738	WP_011836039.1	metal ABC transporter ATP-binding protein	-
RF668_RS04205 (RF668_04205)	-	829905..830714 (+)	810	WP_011677177.1	metal ABC transporter permease	-
RF668_RS04210 (RF668_04210)	-	830736..831608 (-)	873	WP_043736178.1	RluA family pseudouridine synthase	-
RF668_RS04215 (RF668_04215)	-	831803..832180 (+)	378	WP_041168662.1	pyridoxamine 5'-phosphate oxidase family protein	-

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16182.77 Da
• Isoelectric Point: 8.9980

>NTDB_id=876871 RF668_RS04180 WP_374049086.1 826891..827316(+) (comGF) [Lactococcus cremoris strain KCKM 0438]
MKLKIRAFTLLECLVTLLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEANLIKITIDFDNGGERVFIYRFTDTK

Nucleotide

• Length: 426 bp

>NTDB_id=876871 RF668_RS04180 WP_374049086.1 826891..827316(+) (comGF) [Lactococcus cremoris strain KCKM 0438]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTAGAATGTCTCGTAACACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGTCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGCAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACAGATACAAAGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (38-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	98.582	100	0.986
comYF	Streptococcus mutans UA140	43.262	100	0.433
comYF	Streptococcus mutans UA159	42.553	100	0.426
comGF/cglF	Streptococcus mitis NCTC 12261	38.571	99.291	0.383
comGF/cglF	Streptococcus mitis SK321	38.571	99.291	0.383
comGF/cglF	Streptococcus pneumoniae Rx1	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae D39	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae R6	37.143	99.291	0.369
comGF/cglF	Streptococcus pneumoniae TIGR4	37.143	99.291	0.369