Detailed information NTG0876867

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: RF668_RS04160
• Genome accession: NZ_CP133787
• Coordinates: 824829..825854 (+)
• Length: 341 a.a.
• NCBI ID: WP_052206589.1
• Uniprot ID: A0AAX4A9Z3
• Organism: Lactococcus cremoris strain KCKM 0438
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 819829..830854

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RF668_RS04150 (RF668_04150)	-	820061..823834 (+)	3774	Protein_767	PolC-type DNA polymerase III	-
RF668_RS04155 (RF668_04155)	comGA	823949..824929 (+)	981	WP_043736167.1	competence type IV pilus ATPase ComGA	Machinery gene
RF668_RS04160 (RF668_04160)	comGB	824829..825854 (+)	1026	WP_052206589.1	competence type IV pilus assembly protein ComGB	Machinery gene
RF668_RS04165 (RF668_04165)	comGC	825898..826197 (+)	300	WP_052206590.1	competence type IV pilus major pilin ComGC	Machinery gene
RF668_RS04170 (RF668_04170)	comGD	826208..826639 (+)	432	WP_050750513.1	competence type IV pilus minor pilin ComGD	Machinery gene
RF668_RS04175 (RF668_04175)	comGE	826611..826907 (+)	297	WP_043736169.1	competence type IV pilus minor pilin ComGE	Machinery gene
RF668_RS04180 (RF668_04180)	comGF	826891..827316 (+)	426	WP_374049086.1	competence type IV pilus minor pilin ComGF	Machinery gene
RF668_RS04185 (RF668_04185)	comGG	827340..827639 (+)	300	WP_021037947.1	competence type IV pilus minor pilin ComGG	Machinery gene
RF668_RS04190 (RF668_04190)	-	827720..828157 (+)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
RF668_RS04195 (RF668_04195)	-	828154..828996 (+)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
RF668_RS04200 (RF668_04200)	-	829175..829912 (+)	738	WP_011836039.1	metal ABC transporter ATP-binding protein	-
RF668_RS04205 (RF668_04205)	-	829905..830714 (+)	810	WP_011677177.1	metal ABC transporter permease	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38471.23 Da
• Isoelectric Point: 10.0626

>NTDB_id=876867 RF668_RS04160 WP_052206589.1 824829..825854(+) (comGB) [Lactococcus cremoris strain KCKM 0438]
MQTDILRLLRPKGKKLALIKQAKLIQLMGNLLNNGFHLGEVINFLALSKLVEKEFVFKMHQGLSSGNSLSEILESLSFSK
NVVTQLALVEVHGNLVGTMQLVELHLKKQLKVKKKLIEVATYPVVLFVFLIGIMWGLKNYLLPQIDKGNNFATLLINHLP
LVFFSVGALIVLLTSLSIIIFKHLSALTNFTFLVKIPLVSSFVRLYLTAYFSREWGNLIAQGVELRQIIELMKKQKSRIF
AEVGIKLDLGLKGGQNFEQAVSHFSIFLPELSLMIEYGAIKDKLGLELSVYADECWEQFFSKIDRLMQLIQPLIFIFVAL
MIVLLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1026 bp

>NTDB_id=876867 RF668_RS04160 WP_052206589.1 824829..825854(+) (comGB) [Lactococcus cremoris strain KCKM 0438]
ATGCAAACGGACATCTTACGACTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAATGGTTTCCATTTGGGAGAAGTTATCAATTTTTTAGCTTTATCAAAATTAGTAGAAAAAG
AGTTTGTATTCAAAATGCACCAAGGTCTCAGTTCTGGAAATTCATTATCTGAAATTTTAGAGAGTCTCTCTTTTTCTAAA
AATGTTGTGACACAACTTGCTTTGGTAGAAGTGCATGGGAATTTAGTAGGAACCATGCAATTGGTAGAACTGCATTTAAA
AAAGCAGCTTAAGGTCAAAAAGAAATTAATTGAAGTGGCAACTTATCCAGTTGTATTGTTCGTATTTCTCATTGGAATTA
TGTGGGGTTTGAAAAATTATTTATTACCTCAAATTGATAAGGGAAATAATTTTGCTACTTTATTAATTAATCATTTACCG
CTAGTATTTTTTTCTGTTGGTGCTTTAATCGTTTTACTAACAAGTTTATCAATAATAATATTCAAACATCTTTCAGCACT
CACTAATTTTACTTTTTTGGTCAAAATTCCATTAGTAAGTTCTTTTGTTCGCTTGTATTTGACCGCTTATTTCTCCAGAG
AATGGGGAAATTTAATTGCTCAAGGCGTTGAATTGCGACAAATAATAGAATTGATGAAAAAGCAGAAAAGTCGCATTTTT
GCGGAAGTTGGAATTAAACTTGATTTGGGTTTGAAGGGTGGTCAGAATTTTGAGCAAGCTGTCAGTCATTTTTCGATTTT
TTTACCAGAACTTTCTTTGATGATTGAGTACGGCGCGATTAAAGATAAATTAGGGTTGGAGCTTTCTGTTTATGCTGATG
AATGTTGGGAACAATTCTTTTCTAAGATAGACCGTCTAATGCAATTAATACAACCCCTTATTTTTATTTTTGTTGCTTTG
ATGATTGTCTTATTGTATGCAGCAATGTTGTTACCCATTTATTCAAATATGGGTTCTGGAATTTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (211-332)

• Type II secretion system (T2SS), protein F (T2SSF) (25-143)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	97.654	100	0.977
comYB	Streptococcus gordonii str. Challis substr. CH1	51.929	98.827	0.513
comGB/cglB	Streptococcus mitis SK321	51.497	97.947	0.504
comGB/cglB	Streptococcus pneumoniae Rx1	50.898	97.947	0.499
comGB/cglB	Streptococcus pneumoniae D39	50.898	97.947	0.499
comGB/cglB	Streptococcus pneumoniae R6	50.898	97.947	0.499
comGB/cglB	Streptococcus pneumoniae TIGR4	50.898	97.947	0.499
comGB/cglB	Streptococcus mitis NCTC 12261	50.599	97.947	0.496
comYB	Streptococcus mutans UA140	51.592	92.082	0.475
comYB	Streptococcus mutans UA159	50.955	92.082	0.469