Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbB   Type   Machinery gene
Locus tag   RFB12_RS04625 Genome accession   NZ_CP133588
Coordinates   867985..868329 (-) Length   114 a.a.
NCBI ID   WP_015865176.1    Uniprot ID   A0A6G9J011
Organism   Parageobacillus toebii strain NEB857     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 862985..873329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFB12_RS04605 (RFB12_04605) wecB 863224..864369 (-) 1146 WP_309218597.1 non-hydrolyzing UDP-N-acetylglucosamine 2-epimerase -
  RFB12_RS04610 (RFB12_04610) - 864796..866163 (-) 1368 WP_309218598.1 peptidoglycan-binding protein -
  RFB12_RS04615 (RFB12_04615) - 866692..867186 (-) 495 WP_309218599.1 hypothetical protein -
  RFB12_RS04620 (RFB12_04620) - 867398..867811 (+) 414 WP_309218600.1 helix-turn-helix transcriptional regulator -
  RFB12_RS04625 (RFB12_04625) ssbB 867985..868329 (-) 345 WP_015865176.1 single-stranded DNA-binding protein Machinery gene
  RFB12_RS04630 (RFB12_04630) - 868664..869110 (+) 447 WP_015865177.1 YwpF-like family protein -
  RFB12_RS04635 (RFB12_04635) - 869121..869723 (-) 603 WP_062755553.1 class D sortase -
  RFB12_RS04640 (RFB12_04640) - 869729..870694 (-) 966 WP_309218601.1 processed acidic surface protein -
  RFB12_RS04645 (RFB12_04645) - 870827..872203 (-) 1377 WP_309218602.1 aspartate kinase -
  RFB12_RS04650 (RFB12_04650) - 872308..872775 (-) 468 WP_309218603.1 DUF1284 domain-containing protein -

Sequence

Protein


Download         Length: 114 a.a.        Molecular weight: 12796.58 Da        Isoelectric Point: 9.0691

>NTDB_id=875213 RFB12_RS04625 WP_015865176.1 867985..868329(-) (ssbB) [Parageobacillus toebii strain NEB857]
MINQVVLVGRLTKDPELRYTADGAAVANITLAVTRNFRNAEGGIDTDFVQCTLWRKVAENTANYCRKGSIIGVMGRIQTR
NYEKKDGQRVYVTEVVAESVRFMGGKTNEWIEHA

Nucleotide


Download         Length: 345 bp        

>NTDB_id=875213 RFB12_RS04625 WP_015865176.1 867985..868329(-) (ssbB) [Parageobacillus toebii strain NEB857]
ATGATTAATCAAGTCGTGCTCGTCGGCCGGTTGACAAAAGATCCGGAACTTCGATATACCGCCGATGGAGCAGCAGTCGC
TAACATTACGTTAGCGGTAACAAGAAATTTTCGCAATGCGGAAGGGGGAATTGATACCGATTTCGTTCAATGTACACTTT
GGCGGAAAGTGGCGGAGAATACAGCCAATTACTGCCGGAAAGGTTCGATTATTGGCGTCATGGGGCGGATTCAGACAAGA
AACTATGAAAAGAAAGATGGGCAACGAGTATACGTGACAGAAGTAGTGGCTGAATCCGTACGGTTTATGGGCGGCAAAAC
GAATGAATGGATCGAACATGCGTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G9J011

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Bacillus subtilis subsp. subtilis str. 168

67.89

95.614

0.649
  ssbA Bacillus subtilis subsp. subtilis str. 168

63.551

93.86

0.596
  ssb Latilactobacillus sakei subsp. sakei 23K

57.944

93.86

0.544
  ssbB Streptococcus sobrinus strain NIDR 6715-7

44.34

92.982

0.412
  ssbB/cilA Streptococcus pneumoniae TIGR4

42.453

92.982

0.395
  ssbB/cilA Streptococcus mitis NCTC 12261

42.453

92.982

0.395
  ssb Vibrio cholerae strain A1552

38.261

100

0.386
  ssbA Streptococcus mutans UA159

42.718

90.351

0.386
  ssbB/cilA Streptococcus pneumoniae Rx1

41.509

92.982

0.386
  ssbB/cilA Streptococcus pneumoniae R6

41.509

92.982

0.386
  ssbB/cilA Streptococcus mitis SK321

41.509

92.982

0.386
  ssbB/cilA Streptococcus pneumoniae D39

41.509

92.982

0.386
  ssbB Lactococcus lactis subsp. cremoris KW2

40

92.105

0.368