Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RCF95_RS06005 Genome accession   NZ_CP133197
Coordinates   994857..996014 (-) Length   385 a.a.
NCBI ID   WP_308874379.1    Uniprot ID   -
Organism   Thiothrix subterranea strain DNT53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 989857..1001014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF95_RS05985 - 990774..992267 (+) 1494 WP_308135878.1 NADH-quinone oxidoreductase subunit M -
  RCF95_RS05990 - 992270..992788 (-) 519 WP_308135877.1 DUF1566 domain-containing protein -
  RCF95_RS05995 bioD 993074..993733 (+) 660 WP_308135876.1 dethiobiotin synthase -
  RCF95_RS06000 - 993730..994860 (-) 1131 WP_308874378.1 PilT/PilU family type 4a pilus ATPase -
  RCF95_RS06005 pilU 994857..996014 (-) 1158 WP_308874379.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RCF95_RS06010 - 996081..997114 (-) 1034 Protein_1179 type IV pilus twitching motility protein PilT -
  RCF95_RS06015 - 997176..997862 (+) 687 WP_308135871.1 YggS family pyridoxal phosphate-dependent enzyme -
  RCF95_RS06020 - 997869..998366 (-) 498 WP_308135870.1 DUF4124 domain-containing protein -
  RCF95_RS06025 - 998750..999388 (+) 639 WP_308874380.1 (Fe-S)-binding protein -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 43081.74 Da        Isoelectric Point: 7.8512

>NTDB_id=872418 RCF95_RS06005 WP_308874379.1 994857..996014(-) (pilU) [Thiothrix subterranea strain DNT53]
MDRDAAVSYVHKLLATMLEKKASDLYVTAEAARSAKIASEMVPLTNQPLNEQTARMLVRAIMNDRQLKQFEEEMECNFSI
SLPGKARFRVNAFTQRGCAGMVLRHIPNHIPGLKELGLPPVLRDIAMTKRGLVIMVGATGSGKSTTLASMVDFRNTNSKG
HIVTIEDPIEFVHQHKGCLITQREVGVDTSSYEIAMKNTLRQAPDVILLGEIRDREAMDYAIAYAETGHLCLSTLHANSS
NQAIDRIINFFPEERRNQLLMDLSLNLKAVVSQRLVRKAKGEGRLPAVEVVINTPLMADLILKGDVPGMKVLTSKSREHG
MRTFDQALFDLIEARQITVHEALRHADSQNDLRLRLKLESRFAQDNDFFKGLDELAIEPLENATR

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=872418 RCF95_RS06005 WP_308874379.1 994857..996014(-) (pilU) [Thiothrix subterranea strain DNT53]
ATGGATCGCGATGCTGCTGTCAGCTACGTGCATAAGTTATTGGCGACTATGTTGGAAAAAAAGGCTTCCGACTTGTACGT
TACGGCGGAGGCGGCTCGTTCTGCCAAAATTGCCAGCGAAATGGTGCCGTTAACCAATCAGCCCTTGAATGAGCAAACGG
CGCGGATGTTGGTGCGGGCGATTATGAATGACCGCCAGCTCAAACAGTTCGAGGAGGAAATGGAATGCAACTTTTCCATT
AGTCTGCCGGGCAAAGCGCGATTCCGGGTAAACGCTTTCACGCAACGCGGTTGTGCGGGAATGGTGTTGCGCCATATTCC
TAACCATATTCCCGGTTTGAAAGAGTTGGGTTTGCCGCCGGTATTGCGCGATATTGCGATGACCAAACGCGGTTTGGTGA
TTATGGTGGGGGCGACCGGTTCGGGTAAATCGACCACATTGGCGTCAATGGTCGATTTCCGCAATACCAATTCCAAAGGG
CATATTGTCACGATTGAAGACCCGATTGAGTTTGTGCATCAGCACAAAGGTTGCTTGATTACACAGCGCGAAGTGGGGGT
GGATACCAGCAGTTATGAAATTGCGATGAAAAATACCTTGCGCCAAGCCCCTGATGTGATTTTGCTAGGCGAAATTCGGG
ATCGCGAGGCGATGGATTACGCGATTGCTTACGCCGAAACCGGGCATTTGTGCTTGTCGACCTTGCACGCCAACAGTTCC
AACCAAGCGATTGACCGCATTATCAACTTTTTCCCGGAAGAACGCCGCAACCAGTTATTGATGGATTTATCCCTCAACCT
CAAGGCGGTGGTGTCACAGCGGCTGGTGCGTAAAGCCAAAGGGGAAGGGCGTTTGCCAGCGGTGGAAGTGGTGATTAATA
CCCCATTAATGGCGGATTTAATTCTGAAAGGCGATGTGCCGGGCATGAAAGTGCTGACCTCAAAATCGCGCGAACACGGG
ATGCGCACCTTCGACCAAGCGCTATTTGATTTGATTGAAGCGCGGCAAATCACCGTGCATGAAGCGTTGCGTCATGCCGA
TTCGCAGAATGACTTGCGCTTACGCCTCAAATTGGAAAGCCGGTTTGCGCAAGATAATGACTTCTTTAAGGGGTTGGATG
AGTTGGCGATTGAGCCGCTGGAGAATGCCACACGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.739

90.649

0.532
  pilU Acinetobacter baylyi ADP1

55.908

90.13

0.504
  pilU Vibrio cholerae strain A1552

51.149

90.39

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

87.532

0.392
  pilT Acinetobacter baumannii D1279779

42.941

88.312

0.379
  pilT Acinetobacter baumannii strain A118

42.941

88.312

0.379
  pilT Acinetobacter nosocomialis M2

42.647

88.312

0.377
  pilT Vibrio cholerae strain A1552

40.974

90.649

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.974

90.649

0.371
  pilT Legionella pneumophila strain Lp02

42.643

86.494

0.369
  pilT Legionella pneumophila strain ERS1305867

42.643

86.494

0.369
  pilT Acinetobacter baylyi ADP1

43.963

83.896

0.369