Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LLUC029_RS04315 Genome accession   NZ_CP129163
Coordinates   835885..836310 (+) Length   141 a.a.
NCBI ID   WP_032950583.1    Uniprot ID   -
Organism   Lactococcus cremoris strain UC029     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 828921..847614 835885..836310 within 0


Gene organization within MGE regions


Location: 828921..847614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLUC029_RS04255 (LLUC029_04260) - 828921..829811 (-) 891 WP_139949744.1 IS982 family transposase -
  LLUC029_RS04260 (LLUC029_04265) - 829884..830774 (-) 891 WP_231099708.1 site-specific integrase -
  LLUC029_RS04265 (LLUC029_04270) - 830824..831636 (-) 813 WP_050595598.1 S24 family peptidase -
  LLUC029_RS04270 (LLUC029_04275) - 831899..832102 (+) 204 WP_021165564.1 putative transcriptional regulator -
  LLUC029_RS04275 (LLUC029_04280) - 832443..832682 (+) 240 WP_023349341.1 helix-turn-helix transcriptional regulator -
  LLUC029_RS04280 (LLUC029_04285) - 832696..833421 (+) 726 WP_021165562.1 ORF6C domain-containing protein -
  LLUC029_RS04285 (LLUC029_04290) - 833434..833670 (+) 237 WP_011834775.1 helix-turn-helix domain-containing protein -
  LLUC029_RS04290 (LLUC029_04295) - 833824..834057 (+) 234 WP_011834776.1 hypothetical protein -
  LLUC029_RS04295 (LLUC029_04300) - 834012..834290 (-) 279 WP_396425096.1 hypothetical protein -
  LLUC029_RS04300 (LLUC029_04305) - 834393..834629 (+) 237 WP_021165561.1 DUF1408 domain-containing protein -
  LLUC029_RS04305 (LLUC029_04310) - 834727..835125 (+) 399 WP_032950580.1 hypothetical protein -
  LLUC029_RS04310 (LLUC029_04315) - 835134..835892 (+) 759 WP_032950582.1 Rad52/Rad22 family DNA repair protein -
  LLUC029_RS04315 (LLUC029_04320) ssb 835885..836310 (+) 426 WP_032950583.1 single-stranded DNA-binding protein Machinery gene
  LLUC029_RS04320 (LLUC029_04325) - 836438..837244 (+) 807 WP_063282457.1 phage replisome organizer N-terminal domain-containing protein -
  LLUC029_RS04325 (LLUC029_04330) - 837254..838144 (+) 891 WP_021165556.1 ATP-binding protein -
  LLUC029_RS04330 (LLUC029_04335) - 838153..838317 (+) 165 WP_021165555.1 hypothetical protein -
  LLUC029_RS04335 (LLUC029_04340) - 838307..838726 (+) 420 WP_050595599.1 hypothetical protein -
  LLUC029_RS04340 (LLUC029_04345) - 838727..838965 (+) 239 Protein_845 DUF1031 family protein -
  LLUC029_RS04345 (LLUC029_04350) - 839288..839629 (+) 342 WP_058212883.1 hypothetical protein -
  LLUC029_RS04350 (LLUC029_04355) - 839601..839990 (+) 390 WP_032950595.1 YopX family protein -
  LLUC029_RS04355 (LLUC029_04360) - 839987..840193 (+) 207 WP_032950598.1 DUF1125 domain-containing protein -
  LLUC029_RS04360 (LLUC029_04365) - 840186..840761 (+) 576 WP_032950601.1 DUF1642 domain-containing protein -
  LLUC029_RS04365 (LLUC029_04370) istA 840886..842109 (+) 1224 WP_003331415.1 IS21-like element IS712 family transposase -
  LLUC029_RS04370 (LLUC029_04375) istB 842121..842879 (+) 759 WP_003331414.1 IS21-like element IS712 family helper ATPase IstB -
  LLUC029_RS04375 (LLUC029_04380) - 842961..843068 (+) 108 Protein_852 LysM peptidoglycan-binding domain-containing protein -
  LLUC029_RS04380 (LLUC029_04385) - 843143..844527 (+) 1385 WP_332372292.1 IS3 family transposase -
  LLUC029_RS04385 (LLUC029_04390) - 844803..844964 (+) 162 WP_268743976.1 bacteriophage holin -
  LLUC029_RS04395 (LLUC029_04400) - 845793..845993 (+) 201 WP_011676499.1 hypothetical protein -
  LLUC029_RS04400 (LLUC029_04405) - 845975..846865 (-) 891 WP_081196073.1 IS982-like element IS982B family transposase -
  LLUC029_RS04405 (LLUC029_04410) - 847080..847304 (+) 225 WP_032950344.1 garvicin Q family class II bacteriocin -
  LLUC029_RS04410 (LLUC029_04415) - 847309..847614 (+) 306 WP_011834820.1 bacteriocin immunity protein -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15656.42 Da        Isoelectric Point: 5.1972

>NTDB_id=851340 LLUC029_RS04315 WP_032950583.1 835885..836310(+) (ssb) [Lactococcus cremoris strain UC029]
MINNVILVGRITKEPELRYTPQNKAVAAFTLAVNRAFKNANGEREADFINCVIWGKSAENLANWTHKGQLIGVTGSIQTR
NYENQQGQRVYITEVVASNFQVLEKSNQANGERVSNPAAKPQNNDSFGSDPMEISDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=851340 LLUC029_RS04315 WP_032950583.1 835885..836310(+) (ssb) [Lactococcus cremoris strain UC029]
ATGATTAACAATGTCATTCTAGTAGGGCGAATCACTAAAGAACCTGAACTTAGATATACACCACAAAATAAAGCAGTTGC
CGCTTTTACTCTTGCAGTTAATCGAGCATTTAAAAACGCTAATGGAGAAAGAGAAGCTGACTTTATCAATTGTGTTATTT
GGGGTAAATCAGCCGAAAACTTGGCCAATTGGACTCATAAAGGTCAATTAATTGGAGTTACTGGTAGTATTCAAACTCGC
AACTATGAGAATCAACAAGGGCAACGGGTTTATATTACGGAGGTTGTCGCAAGTAATTTCCAAGTACTAGAAAAAAGTAA
TCAAGCAAATGGTGAACGAGTTAGTAATCCAGCTGCAAAACCACAAAATAACGATTCTTTTGGAAGTGATCCAATGGAAA
TTTCAGATGATGACCTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

53.529

100

0.645

  ssbA Bacillus subtilis subsp. subtilis str. 168

51.744

100

0.631

  ssbB/cilA Streptococcus pneumoniae TIGR4

44.681

100

0.447

  ssbB Streptococcus sobrinus strain NIDR 6715-7

44.681

100

0.447

  ssbB/cilA Streptococcus mitis NCTC 12261

44.681

100

0.447

  ssbB/cilA Streptococcus pneumoniae Rx1

43.972

100

0.44

  ssbB/cilA Streptococcus pneumoniae D39

43.972

100

0.44

  ssbB/cilA Streptococcus pneumoniae R6

43.972

100

0.44

  ssbB/cilA Streptococcus mitis SK321

43.972

100

0.44

  ssbB Bacillus subtilis subsp. subtilis str. 168

57.692

73.759

0.426

  ssbA Streptococcus mutans UA159

40.426

100

0.404

  ssbB Lactococcus lactis subsp. cremoris KW2

50.485

73.05

0.369

  ssb Vibrio cholerae strain A1552

29.651

100

0.362