Detailed information NTG0850851

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: LLUC320_RS11095
• Genome accession: NZ_CP129095
• Coordinates: 2158373..2159353 (-)
• Length: 326 a.a.
• NCBI ID: WP_162494683.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain UC320
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2151123..2159353	2158373..2159353	within	0

Gene organization within MGE regions

Location: 2151123..2159353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC320_RS11045 (LLUC320_11105)	-	2152589..2153398 (-)	810	WP_011677177.1	metal ABC transporter permease	-
LLUC320_RS11050 (LLUC320_11110)	-	2153391..2154128 (-)	738	WP_011677178.1	metal ABC transporter ATP-binding protein	-
LLUC320_RS11055 (LLUC320_11115)	-	2154307..2155149 (-)	843	WP_011677179.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
LLUC320_RS11060 (LLUC320_11120)	-	2155146..2155583 (-)	438	WP_011677180.1	zinc-dependent MarR family transcriptional regulator	-
LLUC320_RS11065 (LLUC320_11125)	comGG	2155663..2155890 (-)	228	WP_228764408.1	competence protein ComGG	Machinery gene
LLUC320_RS11070 (LLUC320_11130)	comGF	2155986..2156411 (-)	426	WP_014735174.1	competence type IV pilus minor pilin ComGF	Machinery gene
LLUC320_RS11075 (LLUC320_11135)	comGE	2156395..2156631 (-)	237	WP_014573335.1	competence type IV pilus minor pilin ComGE	Machinery gene
LLUC320_RS11080 (LLUC320_11140)	comGD	2156663..2156851 (-)	189	WP_014573336.1	hypothetical protein	Machinery gene
LLUC320_RS11085 (LLUC320_11145)	comGC	2157053..2157430 (-)	378	Protein_2156	competence type IV pilus major pilin ComGC	-
LLUC320_RS11090 (LLUC320_11150)	comGB	2157448..2158473 (-)	1026	WP_050574185.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLUC320_RS11095 (LLUC320_11155)	comGA	2158373..2159353 (-)	981	WP_162494683.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 37019.32 Da
• Isoelectric Point: 5.9464

>NTDB_id=850851 LLUC320_RS11095 WP_162494683.1 2158373..2159353(-) (comGA) [Lactococcus cremoris strain UC320]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFSGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide

• Length: 981 bp

>NTDB_id=850851 LLUC320_RS11095 WP_162494683.1 2158373..2159353(-) (comGA) [Lactococcus cremoris strain UC320]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTTCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTGAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-265)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Lactococcus lactis subsp. cremoris KW2	99.08	100	0.991
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	56.452	95.092	0.537
comYA	Streptococcus gordonii str. Challis substr. CH1	54.43	96.933	0.528
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	54.487	95.706	0.521
comGA/cglA/cilD	Streptococcus pneumoniae D39	54.487	95.706	0.521
comGA/cglA/cilD	Streptococcus pneumoniae R6	54.487	95.706	0.521
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	54.487	95.706	0.521
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	54.487	95.706	0.521
comYA	Streptococcus mutans UA159	52.733	95.399	0.503
comYA	Streptococcus mutans UA140	52.733	95.399	0.503