Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SrhCFBP13503_RS02745 Genome accession   NZ_CP128598
Coordinates   626937..628070 (-) Length   377 a.a.
NCBI ID   WP_137191128.1    Uniprot ID   -
Organism   Stenotrophomonas rhizophila strain CFBP 13503     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 621937..633070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SrhCFBP13503_RS02730 (SrhCFBP13503_02725) - 622945..624348 (-) 1404 WP_137191131.1 DEAD/DEAH box helicase -
  SrhCFBP13503_RS02735 (SrhCFBP13503_02730) - 625071..626060 (+) 990 WP_038690660.1 fumarylacetoacetate hydrolase family protein -
  SrhCFBP13503_RS02740 (SrhCFBP13503_02735) maiA 626090..626734 (+) 645 WP_181406216.1 maleylacetoacetate isomerase -
  SrhCFBP13503_RS02745 (SrhCFBP13503_02740) pilU 626937..628070 (-) 1134 WP_137191128.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SrhCFBP13503_RS02750 (SrhCFBP13503_02745) - 628225..628566 (+) 342 WP_051598900.1 DUF4398 domain-containing protein -
  SrhCFBP13503_RS02755 (SrhCFBP13503_02750) - 628566..629477 (+) 912 WP_181406210.1 hypothetical protein -
  SrhCFBP13503_RS02760 (SrhCFBP13503_02755) - 629872..630090 (+) 219 WP_038690653.1 YdcH family protein -
  SrhCFBP13503_RS02765 (SrhCFBP13503_02760) - 630292..631662 (+) 1371 WP_137191127.1 pyridoxal-phosphate dependent enzyme -
  SrhCFBP13503_RS02770 (SrhCFBP13503_02765) - 631793..632983 (+) 1191 WP_141058499.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41576.86 Da        Isoelectric Point: 6.2385

>NTDB_id=849238 SrhCFBP13503_RS02745 WP_137191128.1 626937..628070(-) (pilU) [Stenotrophomonas rhizophila strain CFBP 13503]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPEFERELELNMAIALAEAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRAVVSQRLVKGKDGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRMAKNGIIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSANSAPASITHGF

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=849238 SrhCFBP13503_RS02745 WP_137191128.1 626937..628070(-) (pilU) [Stenotrophomonas rhizophila strain CFBP 13503]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAAAAGAACGCGTCGGACATGTTCTTGACCACCGGCGCCCCGGTCTA
CATCAAGATCGAGGGCAAGCTGTACCCGTTGGGGAACACCGGCTTGCCCCCGGGCATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCCGAGTTCGAGCGCGAGCTGGAGCTGAACATGGCCATCGCCCTGGCCGAGGCCGGGCGT
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGTGCGATCCGCAGCAAGATCCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGCTCGACCGGTT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGCGAGGTGGGGCTGGATACGCACGCCTTCCACAA
CGCACTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAAACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGGCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACCGCAACGTGCTGATGAACCTGTCGCTGAACCTGCGCGCGGTGGTGTCCCAGCG
CCTGGTGAAGGGCAAGGACGGGCGCCGCCTGCCGGCCACCGAGGTGTTGATCAACACCCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAGGCCTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGATGGCCAAGAACGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGGCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCAGCGGCGAGCACGATCCGTACGCGGATTTCTCGGCCAACAGCGCCCCGGCGTCGATCA
CCCACGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Acinetobacter baylyi ADP1

50.282

93.899

0.472
  pilU Vibrio cholerae strain A1552

48.611

95.491

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.591

100

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.716

89.655

0.374
  pilT Legionella pneumophila strain Lp02

41.716

89.655

0.374
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter baylyi ADP1

41.176

90.186

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.655

0.369