Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QSH61_RS03640 Genome accession   NZ_CP127855
Coordinates   847425..848546 (+) Length   373 a.a.
NCBI ID   WP_206471375.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. JC3     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 842425..853546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QSH61_RS03610 (QSH61_03610) - 843226..843819 (-) 594 WP_174170327.1 XTP/dITP diphosphatase -
  QSH61_RS03615 (QSH61_03615) - 843827..844261 (-) 435 WP_010607717.1 DUF4426 domain-containing protein -
  QSH61_RS03620 (QSH61_03620) - 844272..844805 (-) 534 WP_010607716.1 YggT family protein -
  QSH61_RS03625 (QSH61_03625) proC 844815..845636 (-) 822 WP_174170326.1 pyrroline-5-carboxylate reductase -
  QSH61_RS03630 (QSH61_03630) - 845650..846333 (-) 684 WP_010607714.1 YggS family pyridoxal phosphate-dependent enzyme -
  QSH61_RS03635 (QSH61_03635) pilT 846364..847410 (+) 1047 WP_010379026.1 type IV pilus twitching motility protein PilT Machinery gene
  QSH61_RS03640 (QSH61_03640) pilU 847425..848546 (+) 1122 WP_206471375.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QSH61_RS03645 (QSH61_03645) - 848524..849411 (+) 888 WP_206471374.1 hypothetical protein -
  QSH61_RS03650 (QSH61_03650) ruvX 849730..850179 (-) 450 WP_017217006.1 Holliday junction resolvase RuvX -
  QSH61_RS03655 (QSH61_03655) - 850222..850779 (-) 558 WP_010607710.1 YqgE/AlgH family protein -
  QSH61_RS03660 (QSH61_03660) gshB 850799..851758 (-) 960 WP_010379037.1 glutathione synthase -
  QSH61_RS03665 (QSH61_03665) rsmE 851816..852550 (-) 735 WP_206471373.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QSH61_RS03670 (QSH61_03670) - 852613..853110 (-) 498 WP_010607708.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41174.11 Da        Isoelectric Point: 6.4182

>NTDB_id=844789 QSH61_RS03640 WP_206471375.1 847425..848546(+) (pilU) [Pseudoalteromonas sp. JC3]
MNIEPFLQTMANQQASDLFVSAGLAVSAKIDGELRALSEERLDAAQSLNLVTSIMSDKQKQEFFNTKECNFAIANDIGRF
RVSAFWQRDCAGMVIRRIVTAIPDVNELGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIEDP
IEFVHQHQKSIITQREVGIDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRIM
HLVPKEKHDKLKYDLALNLRAIVAQQLVPSSKGEGREAAIEVLLNSPLVAELIKKGDIGSIKEVMAKSKDMGMQTFDQAL
FELYKQQRINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTVDGLDSKN

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=844789 QSH61_RS03640 WP_206471375.1 847425..848546(+) (pilU) [Pseudoalteromonas sp. JC3]
ATGAATATAGAACCGTTTTTGCAAACGATGGCCAATCAGCAAGCATCCGACTTATTCGTTTCGGCAGGGCTTGCGGTGAG
CGCCAAAATCGATGGCGAACTTAGAGCCTTAAGTGAAGAAAGACTGGATGCCGCGCAGTCACTGAATCTTGTTACCTCGA
TTATGAGCGACAAGCAAAAGCAAGAATTCTTTAACACTAAAGAGTGCAACTTTGCGATTGCTAACGATATCGGGCGTTTT
CGTGTTTCAGCATTTTGGCAACGGGATTGTGCGGGCATGGTTATCCGCCGTATCGTTACTGCTATTCCTGATGTCAATGA
GCTAGGTTTGCCGTCGGTACTGACCGATGTAATCATGTCCAAACGTGGCTTGGTGTTATTTGTAGGTGGTACGGGCACGG
GTAAGTCAACTTCGCTTGCAGCACTGCTCGGTTATCGTAATCGTAACCAACGTGGCCATATTTTGACCATTGAAGATCCC
ATCGAATTTGTGCATCAACACCAAAAAAGCATCATTACTCAGCGTGAGGTGGGCATTGATACTGACAGTTTTGAATCTGC
CTTAAAAAGTTCATTACGTCAAGCGCCAGATGTTATTTTAATTGGTGAAATTCGCTCGCAAGAAACCATGGAGTATGCAC
TGAGCTTTGCTGAAACGGGGCATTTATGCGTTGCAACCTTGCACGCCAATAATGCTAACCAAGCCATCGACCGTATCATG
CACTTAGTCCCCAAAGAAAAGCACGATAAACTGAAGTACGATTTGGCGCTGAACTTGCGCGCTATTGTGGCGCAACAACT
AGTGCCTTCTTCAAAAGGCGAAGGTCGAGAGGCGGCCATTGAAGTGTTACTGAACTCCCCACTCGTTGCCGAGCTTATTA
AAAAAGGCGATATTGGCTCGATTAAAGAGGTAATGGCAAAATCGAAAGACATGGGCATGCAAACCTTTGATCAAGCGTTG
TTTGAGCTTTATAAGCAGCAGCGGATCAACTATGCCGACGCACTACATCATGCTGACTCGCCAAACGATCTGCGCTTGAT
GATCAAGCTGCAAAATAATGAACAAAAAGGTGCGGGCTTCCTACAAGGGGTTACGGTAGATGGGTTGGATTCGAAGAACT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

56.764

100

0.574
  pilU Pseudomonas stutzeri DSM 10701

60.114

94.102

0.566
  pilU Vibrio cholerae strain A1552

56.403

98.391

0.555
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.778

96.515

0.413
  pilT Acinetobacter baylyi ADP1

40.936

91.689

0.375
  pilT Legionella pneumophila strain ERS1305867

41.742

89.276

0.373
  pilT Legionella pneumophila strain Lp02

41.742

89.276

0.373
  pilT Pseudomonas aeruginosa PAK

40

92.493

0.37
  pilT Acinetobacter nosocomialis M2

40.058

91.689

0.367
  pilT Acinetobacter baumannii D1279779

40.058

91.689

0.367
  pilT Acinetobacter baumannii strain A118

40.058

91.689

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.42

92.493

0.365