Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QP436_RS15760 Genome accession   NZ_CP127404
Coordinates   3525060..3526187 (-) Length   375 a.a.
NCBI ID   WP_100554661.1    Uniprot ID   A0AAW4GKF4
Organism   Stenotrophomonas sp. As-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3520060..3531187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP436_RS15740 - 3520489..3521478 (+) 990 WP_191078366.1 fumarylacetoacetate hydrolase family protein -
  QP436_RS15745 maiA 3521493..3522161 (+) 669 WP_205405354.1 maleylacetoacetate isomerase -
  QP436_RS15750 - 3522195..3523667 (-) 1473 WP_205405353.1 UdgX family uracil-DNA binding protein -
  QP436_RS15755 - 3523648..3524931 (-) 1284 WP_205405352.1 putative DNA modification/repair radical SAM protein -
  QP436_RS15760 pilU 3525060..3526187 (-) 1128 WP_100554661.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QP436_RS15765 - 3526309..3526674 (+) 366 WP_089238584.1 DUF4398 domain-containing protein -
  QP436_RS15770 - 3526671..3527555 (+) 885 WP_205405351.1 hypothetical protein -
  QP436_RS15775 - 3527879..3528115 (+) 237 WP_093818802.1 YdcH family protein -
  QP436_RS15780 - 3528419..3529789 (+) 1371 WP_180166682.1 pyridoxal-phosphate dependent enzyme -
  QP436_RS15785 - 3529913..3531097 (+) 1185 WP_205405350.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41388.62 Da        Isoelectric Point: 6.2378

>NTDB_id=844077 QP436_RS15760 WP_100554661.1 3525060..3526187(-) (pilU) [Stenotrophomonas sp. As-6]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFHDALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKNGTIDQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=844077 QP436_RS15760 WP_100554661.1 3525060..3526187(-) (pilU) [Stenotrophomonas sp. As-6]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTGTATCCGCTCGGCAATACCGGCCTGCCGCCGGGAATGGTGAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGGCCGATGCCGGGCGT
TTCCGAGTCAACGTGTTCAAGCAGCGCGGTGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACTCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGATCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAACACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTCGATACCCACACCTTCCACGA
CGCGCTGAAGAACGCGATGCGTGAAGCACCCGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CAATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCCACCCTGCATTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGGTCAGCCAGCG
TCTGGTGAAGAACAAGGAGGGGCGCCGCCTGCCAGCCACCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCAGCAATGGAGGCGTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGTTTGGCCAAGAACGGCACCATCGATCAGGAAGAGGCACTGCGTGCGGCCGACTCCCGCGATGGCCTGGCGCTGAA
ATTCCGCCTGTCCGAAGGCGGCAGCGGCGAGCATGATCCGTATGCGGACTTCTCGGCCGGCGCGCCGAGCATTACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

95.733

0.493
  pilU Vibrio cholerae strain A1552

48.889

96

0.469
  pilU Acinetobacter baylyi ADP1

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.04

99.467

0.448
  pilT Pseudomonas aeruginosa PAK

42.308

90.133

0.381
  pilT Legionella pneumophila strain ERS1305867

41.124

90.133

0.371
  pilT Acinetobacter nosocomialis M2

41.124

90.133

0.371
  pilT Acinetobacter baumannii D1279779

41.124

90.133

0.371
  pilT Acinetobacter baumannii strain A118

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

41.124

90.133

0.371
  pilT Acinetobacter baylyi ADP1

40.882

90.667

0.371
  pilT Pseudomonas stutzeri DSM 10701

40.828

90.133

0.368