Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QN061_RS01785 Genome accession   NZ_CP126305
Coordinates   371371..372477 (+) Length   368 a.a.
NCBI ID   WP_024374564.1    Uniprot ID   -
Organism   Vibrio fluvialis strain SD21SC3889PM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 366371..377477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN061_RS01750 (QN061_01750) - 366721..367320 (-) 600 WP_024374557.1 XTP/dITP diphosphatase -
  QN061_RS01755 (QN061_01755) - 367344..367775 (-) 432 WP_024374558.1 DUF4426 domain-containing protein -
  QN061_RS01760 (QN061_01760) yggU 367835..368125 (-) 291 WP_024374559.1 DUF167 family protein YggU -
  QN061_RS01765 (QN061_01765) - 368125..368682 (-) 558 WP_024374560.1 YggT family protein -
  QN061_RS01770 (QN061_01770) proC 368734..369552 (-) 819 WP_222726002.1 pyrroline-5-carboxylate reductase -
  QN061_RS01775 (QN061_01775) - 369589..370296 (-) 708 WP_284162079.1 YggS family pyridoxal phosphate-dependent enzyme -
  QN061_RS01780 (QN061_01780) pilT 370322..371359 (+) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  QN061_RS01785 (QN061_01785) pilU 371371..372477 (+) 1107 WP_024374564.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QN061_RS01790 (QN061_01790) ruvX 372543..372968 (-) 426 WP_024374565.1 Holliday junction resolvase RuvX -
  QN061_RS01795 (QN061_01795) - 373030..373593 (-) 564 WP_032080468.1 YqgE/AlgH family protein -
  QN061_RS01800 (QN061_01800) gshB 373633..374589 (-) 957 WP_024374566.1 glutathione synthase -
  QN061_RS01805 (QN061_01805) rsmE 374602..375333 (-) 732 WP_032080467.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QN061_RS01810 (QN061_01810) - 375430..376131 (-) 702 WP_032080466.1 endonuclease -
  QN061_RS01815 (QN061_01815) - 376250..376750 (-) 501 WP_143683815.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41203.14 Da        Isoelectric Point: 6.1909

>NTDB_id=837412 QN061_RS01785 WP_024374564.1 371371..372477(+) (pilU) [Vibrio fluvialis strain SD21SC3889PM]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAQGEKLDEAQVSALLAAMMDEARHADYLTTREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPAVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGDLHELKSTMAKSKEVGMQTFDQALY
QLVVENKISEQDALHCADSANDLRLMLKTQRGDVSSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=837412 QN061_RS01785 WP_024374564.1 371371..372477(+) (pilU) [Vibrio fluvialis strain SD21SC3889PM]
ATGCAACTCGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCAGATCTATATATTACTGTGGGCGCCCCCGTACT
GTACCGGGTCGATGGTGAACTGCGCGCGCAGGGTGAGAAGCTGGATGAAGCGCAGGTCAGCGCGCTGCTGGCTGCGATGA
TGGATGAGGCGCGCCATGCGGACTATCTGACGACGCGTGAAGCTAACTTTGCCATCGTGCGTGATTTGGGGCGTTTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTACCCGGTGCGGTGATTCGCCGCATCGAAACGCAGATTCCGACCTTTGATGAACT
CAAACTGCCAGCGGTGCTGAAAGATCTCTCGATTGCCAAACGCGGTTTAGTGCTGGTGGTGGGGGCGACAGGGTCGGGTA
AATCGACCACCATGGCAGCGATGACTGGCTATCGCAACACGCACCGCAGCGGGCATATTCTCACCGTCGAAGATCCGATT
GAATTTGTCCATGAGCACCAGCGCTGCATCGTGACGCAGCGTGAAGTGGGATTGGACACTGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTACGCCAGGCGCCGGATATGATTTTGATCGGAGAGATCCGCAGCCGTGAAACCATGGAGTACGCAATGA
CCTTCGCCGAAACCGGTCACTTGTGCATGGCCACTCTGCACGCCAACAACGCCAACCAAGCACTTGAACGTATCTTGCAT
CTGGTGCCGAAAGAGCAGCGTGAGCAATTCCTGTTTGATCTGTCGATGAACCTTAAAGGTGTTGTCGGTCAGCAATTGAT
CCGCGATAAAAACGGGCAAGGGCGTCACGGCGTGTTTGAAGTGTTGCTCAACAGTCCACGGGTGTCGGACATCATCCGAC
GCGGCGATTTGCATGAGCTGAAAAGCACCATGGCGAAATCGAAAGAGGTTGGCATGCAGACTTTTGATCAGGCGTTGTAT
CAGTTGGTGGTCGAGAACAAGATCAGCGAACAGGATGCGCTGCACTGTGCCGATTCGGCCAACGATCTGCGCTTGATGCT
CAAGACTCAGCGGGGTGATGTGAGCAGCAGCGGTTCACTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.598

100

0.856
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44

88.315

0.389
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas aeruginosa PAK

41.194

91.033

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.701

91.033

0.361