Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   QIF44_RS18660 Genome accession   NZ_CP124546
Coordinates   4120268..4120681 (-) Length   137 a.a.
NCBI ID   WP_032975177.1    Uniprot ID   -
Organism   Stenotrophomonas indicatrix strain MGMM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4115268..4125681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QIF44_RS18640 (QIF44_18640) - 4115791..4116678 (-) 888 WP_051584899.1 FkbM family methyltransferase -
  QIF44_RS18645 (QIF44_18645) - 4116760..4117872 (+) 1113 WP_032975174.1 glycosyltransferase family 4 protein -
  QIF44_RS18650 (QIF44_18650) - 4117866..4119770 (-) 1905 WP_032975175.1 hypothetical protein -
  QIF44_RS18655 (QIF44_18655) - 4119770..4120207 (-) 438 WP_313114357.1 pilin -
  QIF44_RS18660 (QIF44_18660) pilA/pilAI 4120268..4120681 (-) 414 WP_032975177.1 pilin Machinery gene
  QIF44_RS18665 (QIF44_18665) pilC 4121034..4122293 (+) 1260 WP_032975227.1 type II secretion system F family protein Machinery gene
  QIF44_RS18670 (QIF44_18670) - 4122301..4123164 (+) 864 WP_025874291.1 A24 family peptidase -
  QIF44_RS18675 (QIF44_18675) coaE 4123176..4123787 (+) 612 WP_032975178.1 dephospho-CoA kinase -
  QIF44_RS18680 (QIF44_18680) - 4123840..4124226 (-) 387 WP_025874288.1 hypothetical protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 13969.10 Da        Isoelectric Point: 8.8566

>NTDB_id=826404 QIF44_RS18660 WP_032975177.1 4120268..4120681(-) (pilA/pilAI) [Stenotrophomonas indicatrix strain MGMM10]
MKNQKGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQVTAGLADIRGGVTAFEEGIQSGTKTGSPVAADLGLNSNTPRC
GIAPSGAWSDASGQKIVCTLKGNPKVVGETVTLTRNASGAWNCTASAKLADYKPAGC

Nucleotide


Download         Length: 414 bp        

>NTDB_id=826404 QIF44_RS18660 WP_032975177.1 4120268..4120681(-) (pilA/pilAI) [Stenotrophomonas indicatrix strain MGMM10]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTCGTCGCGATCATCGCGATCCTGGCCGCCATTGCGCT
GCCGGCTTACCAGAACTACGTCGCCAAGTCGCAGGTCACTGCCGGCCTGGCCGACATCCGCGGCGGCGTGACTGCCTTCG
AAGAAGGCATCCAGTCGGGCACCAAAACTGGTAGCCCGGTGGCTGCTGACCTTGGTCTGAACTCCAACACCCCGCGTTGC
GGCATTGCCCCGAGCGGCGCTTGGAGCGATGCTTCTGGCCAGAAGATCGTCTGCACCCTGAAGGGTAACCCGAAGGTCGT
CGGCGAGACCGTCACCCTCACCCGCAACGCTTCCGGTGCCTGGAACTGCACCGCATCGGCCAAGCTGGCTGATTACAAGC
CGGCCGGCTGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.36

100

0.511
  pilA Acinetobacter baumannii strain A118

47.143

100

0.482
  pilA Pseudomonas aeruginosa PAK

42.282

100

0.46
  pilA Vibrio cholerae C6706

40.816

100

0.438
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.816

100

0.438
  pilA Vibrio cholerae strain A1552

40.816

100

0.438
  pilA2 Legionella pneumophila str. Paris

39.189

100

0.423
  pilA2 Legionella pneumophila strain ERS1305867

38.889

100

0.409
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.176

99.27

0.409
  comP Acinetobacter baylyi ADP1

36.486

100

0.394
  pilA Vibrio parahaemolyticus RIMD 2210633

41.27

91.971

0.38