Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QHG62_RS17000 Genome accession   NZ_CP123990
Coordinates   3508792..3509928 (-) Length   378 a.a.
NCBI ID   WP_281146776.1    Uniprot ID   -
Organism   Variovorax paradoxus strain MGMM5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3503792..3514928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QHG62_RS16970 (QHG62_16970) - 3504181..3504909 (+) 729 WP_281146771.1 glutathione S-transferase -
  QHG62_RS16975 (QHG62_16975) rsmI 3504912..3505871 (-) 960 WP_281146772.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  QHG62_RS16980 (QHG62_16980) - 3505917..3506282 (+) 366 WP_281146773.1 YraN family protein -
  QHG62_RS16985 (QHG62_16985) - 3506375..3507019 (+) 645 WP_281146774.1 SIS domain-containing protein -
  QHG62_RS16990 (QHG62_16990) - 3507016..3507786 (+) 771 WP_281146775.1 BON domain-containing protein -
  QHG62_RS16995 (QHG62_16995) - 3507842..3508750 (-) 909 WP_157616145.1 NAD(P)-dependent oxidoreductase -
  QHG62_RS17000 (QHG62_17000) pilU 3508792..3509928 (-) 1137 WP_281146776.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QHG62_RS17005 (QHG62_17005) - 3510001..3510228 (+) 228 Protein_3376 twitching motility protein PilT -
  QHG62_RS17010 (QHG62_17010) pilU 3510256..3511392 (+) 1137 WP_281146776.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QHG62_RS17015 (QHG62_17015) - 3511434..3512342 (+) 909 WP_157616145.1 NAD(P)-dependent oxidoreductase -
  QHG62_RS17020 (QHG62_17020) - 3512398..3513168 (-) 771 WP_281146775.1 BON domain-containing protein -
  QHG62_RS17025 (QHG62_17025) - 3513165..3513809 (-) 645 WP_281146774.1 SIS domain-containing protein -
  QHG62_RS17030 (QHG62_17030) - 3513902..3514267 (-) 366 WP_281146773.1 YraN family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41887.96 Da        Isoelectric Point: 6.4189

>NTDB_id=823623 QHG62_RS17000 WP_281146776.1 3508792..3509928(-) (pilU) [Variovorax paradoxus strain MGMM5]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLTIMVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLIPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=823623 QHG62_RS17000 WP_281146776.1 3508792..3509928(-) (pilU) [Variovorax paradoxus strain MGMM5]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
TACGGCGGACTTTCCGCCCGCCATCAAGGTAGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGGCAAACGGCTGAGTTCGAGCGCACCAAGGAGTGCAACTTTGCGATC
TCGCCCACCGGCATTGGCCGGTTCCGCGTGAACGCTTTCGTGCAGCAGGGCAAGGTGGGCATGGTGCTGCGGACCATTCC
GGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCGATGACCAAGCGCGGCCTCACCA
TCATGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTTGCGGCCATGATCGACTGGCGCAACGAAAACTCCTACGGC
CACATCGTCACGGTGGAAGACCCGGTGGAGTTTGTGCACCCGCACAAGAACTGCGTGGTAACCCAGCGCGAAGTAGGCAT
TGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAAGCGCCCGACGTGATCTTGATGGGCGAAATTCGCG
ACCGCGAAACCATGGAACACGCGGTGGCTTTTGCCGAAACCGGCCACCTTTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTTCCCGAAGAACGCCGCGCGCAGCTGCTGATGGACTTGTCGCTGAACCT
GCGTTCGCTGGTTTCGCAGCGCCTGATTCCTACCGAAGACGGCTTGGGCCGCGTTGCCGCAGTCGAAGTGCTGCTGAACA
CGCCGCTCATCTCCGACCTGATCTTCAAGGGCGAAGTGGGCGAGATCAAGGAAATCATGAGGAAGAGCCGGAACCTCGGC
ATGCAGACCTTCGACCAAGCGCTGTTCGACCTGTTCGAAAGCCACTCGATCACCTTCGAAGACGCCATTCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTTTCGGCCGGCACGGAGC
ATTTCGCGATTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.542

94.709

0.545
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Legionella pneumophila strain ERS1305867

43.465

87.037

0.378
  pilT Legionella pneumophila strain Lp02

43.465

87.037

0.378
  pilT Acinetobacter baumannii D1279779

44.514

84.392

0.376
  pilT Acinetobacter baumannii strain A118

44.514

84.392

0.376
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373