Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QC632_RS22060 Genome accession   NZ_CP123897
Coordinates   4997703..4998863 (-) Length   386 a.a.
NCBI ID   WP_281021538.1    Uniprot ID   -
Organism   Methylomonas sp. UP202     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4992703..5003863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QC632_RS22030 (QC632_22030) lexA 4994042..4994668 (+) 627 WP_071158838.1 transcriptional repressor LexA -
  QC632_RS22035 (QC632_22035) - 4994683..4994832 (+) 150 WP_157197959.1 hypothetical protein -
  QC632_RS22040 (QC632_22040) - 4994855..4995187 (+) 333 WP_281021534.1 NGG1p interacting factor NIF3 -
  QC632_RS22045 (QC632_22045) - 4995821..4996360 (-) 540 WP_281021535.1 hypothetical protein -
  QC632_RS22050 (QC632_22050) - 4996467..4997309 (-) 843 WP_281021536.1 endonuclease/exonuclease/phosphatase family protein -
  QC632_RS22055 (QC632_22055) - 4997340..4997552 (-) 213 WP_281021537.1 DUF6447 family protein -
  QC632_RS22060 (QC632_22060) pilU 4997703..4998863 (-) 1161 WP_281021538.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QC632_RS22065 (QC632_22065) - 4998982..5000025 (+) 1044 WP_071158847.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  QC632_RS22070 (QC632_22070) - 5000022..5000246 (+) 225 WP_281021539.1 sulfurtransferase TusA family protein -
  QC632_RS22075 (QC632_22075) argJ 5000292..5001506 (+) 1215 WP_281021540.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  QC632_RS22080 (QC632_22080) - 5001508..5002464 (+) 957 WP_281021541.1 Nudix family hydrolase -
  QC632_RS22085 (QC632_22085) yacG 5002449..5002652 (-) 204 WP_281021542.1 DNA gyrase inhibitor YacG -
  QC632_RS22090 (QC632_22090) zapD 5002652..5003422 (-) 771 WP_281021543.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 42688.76 Da        Isoelectric Point: 5.6142

>NTDB_id=823006 QC632_RS22060 WP_281021538.1 4997703..4998863(-) (pilU) [Methylomonas sp. UP202]
MEFKDYLKILVQQDGSDLYLTAGAPPAAKFHGTLRPLENIRLTNERLKEIAHAIMDADQRAAFEQVPEMNLAIAEEGVGR
FRVNIFKQRSNYALVIRNIKVDIPNADTLGLPQVLKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSHASGHIITIED
PIEFVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNLQAFVSQRLVPTVEGKRVAAIEILLGSKLVSDLIQKGDVHAIKEAMEKSENIGMQTFDSHL
LKLYKSGVISLDEALRNADSPNNLKLKINLSEGLGSATADDKPSLADTLSLQEIVKPEENEEEGGH

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=823006 QC632_RS22060 WP_281021538.1 4997703..4998863(-) (pilU) [Methylomonas sp. UP202]
ATGGAATTTAAAGATTACCTGAAAATTTTGGTTCAACAAGACGGTTCGGATTTATATCTGACCGCCGGCGCGCCGCCGGC
CGCTAAATTTCACGGCACGCTGCGGCCGCTGGAAAACATCCGGCTGACCAACGAAAGACTTAAAGAGATAGCCCATGCCA
TCATGGATGCCGATCAGCGCGCGGCCTTCGAACAGGTACCGGAAATGAATCTAGCCATCGCCGAGGAAGGCGTGGGCCGG
TTTAGGGTGAATATTTTCAAGCAACGCAGCAATTACGCGCTGGTGATCCGCAACATCAAGGTCGATATTCCCAACGCCGA
CACCCTGGGCTTGCCGCAGGTATTGAAAGACAAAATCATGGAAAAGCGCGGCCTGATCTTGTTCGTCGGCGGCACCGGCT
CCGGCAAATCCACCTCGCTGGCGGCCTTGATCGATTACCGCAACAGCCATGCGTCCGGCCACATCATTACCATCGAAGAC
CCGATCGAGTTCGTCCATCCGCACAAGAAATCGCTGGTGAATCAGCGCGAGGTCGGCGTCGATACGCTCAGCTATGAAGA
CGCGCTGAAAAACACGCTGCGCCAAGCGCCCGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACGATGGAGCACG
CGCTGGCCTTCGCCGAAACCGGCCATCTGTGTCTGTCGACCTTGCATGCCAACAACGCCAATCAGGCCTTGGACCGGATC
ATCAATTTCTTTCCGGAAGAGCGCCGTCCGCAATTGTTGATGGACTTGTCGCTGAACTTGCAGGCCTTCGTGTCGCAACG
CTTGGTGCCGACCGTGGAAGGCAAGCGGGTCGCGGCGATCGAGATATTGTTGGGCAGTAAACTGGTCAGCGACCTGATCC
AAAAAGGCGACGTGCATGCGATCAAGGAAGCCATGGAAAAATCGGAAAACATCGGCATGCAAACCTTCGACAGCCATTTG
CTCAAGCTCTATAAAAGCGGCGTGATTTCGCTGGACGAGGCATTGCGCAACGCCGATTCGCCGAACAACCTAAAGCTCAA
AATCAACCTCAGCGAAGGCCTGGGTTCGGCGACCGCCGACGACAAACCCAGCTTGGCGGATACTTTGTCGTTGCAGGAAA
TCGTCAAGCCCGAGGAAAATGAAGAAGAGGGCGGGCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(68-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.053

97.409

0.536
  pilU Acinetobacter baylyi ADP1

53.39

91.71

0.49
  pilU Vibrio cholerae strain A1552

51.13

91.71

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

93.264

0.391
  pilT Pseudomonas aeruginosa PAK

41.279

89.119

0.368
  pilT Pseudomonas stutzeri DSM 10701

41.279

89.119

0.368
  pilT Acinetobacter baumannii strain A118

40.698

89.119

0.363
  pilT Acinetobacter nosocomialis M2

40.698

89.119

0.363
  pilT Acinetobacter baumannii D1279779

40.698

89.119

0.363
  pilT Acinetobacter baylyi ADP1

40.698

89.119

0.363
  pilT Legionella pneumophila strain ERS1305867

42.424

85.492

0.363
  pilT Legionella pneumophila strain Lp02

42.424

85.492

0.363