Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R5029_RS06415 Genome accession   NZ_CP137522
Coordinates   1326447..1326911 (+) Length   154 a.a.
NCBI ID   WP_317939508.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA0118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1321447..1331911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5029_RS06390 (R5029_06390) yacG 1321612..1321812 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  R5029_RS06395 (R5029_06395) coaE 1321809..1322420 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  R5029_RS06400 (R5029_06400) pilD 1322417..1323289 (-) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R5029_RS06405 (R5029_06405) pilC 1323293..1324513 (-) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  R5029_RS06410 (R5029_06410) pilB 1324517..1326217 (-) 1701 WP_317939510.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5029_RS06415 (R5029_06415) pilA 1326447..1326911 (+) 465 WP_317939508.1 pilin Machinery gene
  R5029_RS06425 (R5029_06425) nadC 1327115..1327963 (-) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R5029_RS06430 (R5029_06430) - 1328249..1330504 (+) 2256 WP_003094643.1 DUF1631 domain-containing protein -
  R5029_RS06435 (R5029_06435) ampD 1330655..1331221 (+) 567 WP_126892710.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15796.95 Da        Isoelectric Point: 6.1571

>NTDB_id=821935 R5029_RS06415 WP_317939508.1 1326447..1326911(+) (pilA) [Pseudomonas aeruginosa strain HPA0118]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVSRSQVSRVMAEAGSLKTAVEACLQDGRTAVGTAAGQCDPGATGSSL
LTGASQTSQTLPTNTGVPQVLDLLTTQTTIIATFGNGASAAISGQTLTWTRDVNGGWSCATTVDAKFRPNGCTD

Nucleotide


Download         Length: 465 bp        

>NTDB_id=821935 R5029_RS06415 WP_317939508.1 1326447..1326911(+) (pilA) [Pseudomonas aeruginosa strain HPA0118]
ATGAAAGCACAGAAGGGTTTTACTCTGATTGAACTGATGATCGTAGTTGCGATCATTGGTATTCTGGCTGCAATCGCAAT
TCCCCAATATCAGAACTATGTTTCTCGCTCTCAGGTCTCCAGGGTTATGGCAGAGGCTGGCTCCTTGAAGACTGCAGTTG
AGGCCTGCCTCCAGGATGGTCGTACTGCTGTGGGTACTGCTGCTGGTCAATGCGACCCGGGTGCGACGGGTTCCAGTTTG
TTGACTGGTGCTTCTCAGACTTCTCAAACCCTGCCAACCAATACCGGTGTTCCGCAGGTTCTGGATCTTCTGACTACTCA
AACCACTATCATTGCGACTTTTGGTAACGGTGCATCCGCAGCTATTTCTGGCCAGACTCTGACCTGGACTCGTGATGTTA
ATGGTGGCTGGAGCTGTGCTACTACCGTAGATGCTAAATTCCGTCCTAATGGCTGTACTGACTGA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-153)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.753

100

0.468
  pilA Vibrio cholerae strain A1552

43.59

100

0.442
  pilA Vibrio cholerae C6706

43.59

100

0.442
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.59

100

0.442
  comP Acinetobacter baylyi ADP1

40.12

100

0.435
  pilA Vibrio parahaemolyticus RIMD 2210633

40.881

100

0.422
  pilA Acinetobacter baumannii strain A118

41.83

99.351

0.416
  pilA2 Legionella pneumophila strain ERS1305867

41.558

100

0.416
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.385

100

0.409
  pilA2 Legionella pneumophila str. Paris

40.909

100

0.409
  pilE Neisseria gonorrhoeae MS11

33.908

100

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.926

96.753

0.377