Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   P8P68_RS06280 Genome accession   NZ_CP121467
Coordinates   1293415..1294356 (-) Length   313 a.a.
NCBI ID   WP_000249575.1    Uniprot ID   -
Organism   Streptococcus sp. D7B5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1288415..1299356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8P68_RS06240 (P8P68_06240) - 1288685..1289996 (+) 1312 Protein_1197 IS3 family transposase -
  P8P68_RS06245 (P8P68_06245) - 1289990..1290616 (-) 627 WP_278275776.1 class I SAM-dependent methyltransferase -
  P8P68_RS06250 (P8P68_06250) comGG/cglG 1290649..1291062 (-) 414 WP_049490203.1 competence type IV pilus minor pilin ComGG Machinery gene
  P8P68_RS06255 (P8P68_06255) comGF/cglF 1291040..1291501 (-) 462 WP_049490202.1 competence type IV pilus minor pilin ComGF Machinery gene
  P8P68_RS06260 (P8P68_06260) comGE/cglE 1291464..1291766 (-) 303 WP_000413367.1 competence type IV pilus minor pilin ComGE Machinery gene
  P8P68_RS06265 (P8P68_06265) comGD/cglD 1291729..1292163 (-) 435 WP_049519116.1 competence type IV pilus minor pilin ComGD Machinery gene
  P8P68_RS06270 (P8P68_06270) comGC/cglC 1292126..1292449 (-) 324 WP_000735798.1 competence type IV pilus major pilin ComGC Machinery gene
  P8P68_RS06275 (P8P68_06275) comGB/cglB 1292451..1293467 (-) 1017 WP_000355379.1 competence type IV pilus assembly protein ComGB Machinery gene
  P8P68_RS06280 (P8P68_06280) comGA/cglA/cilD 1293415..1294356 (-) 942 WP_000249575.1 competence type IV pilus ATPase ComGA Machinery gene
  P8P68_RS06285 (P8P68_06285) - 1294426..1294791 (-) 366 WP_000286396.1 DUF1033 family protein -
  P8P68_RS06290 (P8P68_06290) nagA 1294946..1296097 (-) 1152 WP_001134447.1 N-acetylglucosamine-6-phosphate deacetylase -
  P8P68_RS06295 (P8P68_06295) - 1296238..1298055 (-) 1818 WP_278275777.1 acyltransferase family protein -
  P8P68_RS06300 (P8P68_06300) tgt 1298189..1299331 (-) 1143 WP_001285237.1 tRNA guanosine(34) transglycosylase Tgt -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35475.45 Da        Isoelectric Point: 5.7829

>NTDB_id=812237 P8P68_RS06280 WP_000249575.1 1293415..1294356(-) (comGA/cglA/cilD) [Streptococcus sp. D7B5]
MVQEIAQKIIATAKAKKAQDIYFIPKEKSYELHMRVGDARCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFMGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTRWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=812237 P8P68_RS06280 WP_000249575.1 1293415..1294356(-) (comGA/cglA/cilD) [Streptococcus sp. D7B5]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGCAAAGAAGGCTCAGGATATCTATTTCATTCCCAAGGA
AAAGTCCTATGAGCTTCACATGCGGGTTGGAGACGCGCGGTGTCTCGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTAGCGGGTATGAATGTAGGGGAGAAGAGACGTAGTCAGCTGGGCTCTTGCGACTATCAG
CATGGGGAGAAGGTGTCTTCTCTGCGTTTGTCTACCGTAGGAGATTATCGGGGACATGAGAGTTTGGTCATTCGTTTGTT
GCACGACGAAGAGCAGGACCTGCATTTCTGGTTTCAGGATATGAATGAACTGGGTGAGCAGTACCGGCAACGGGGCCTCT
ACCTTTTTGCGGGTCCAGTTGGAAGTGGCAAGACGACCTTGATGCACGAATTAGCCAAGTCTCTGTTTATGGGGCAACAG
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGAAGACATGCTCCAGTTGCAGTTGAATGAAGCGATTGGCTTGAC
CTATGAAAATCTGATCAAACTGTCTCTCCGGCATCGTCCGGACCTCTTGATTATCGGTGAAATTCGTGACAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCATGCTAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGGGTGACGGAGGAGGAATTAGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACCAGGTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCCGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

88.818

100

0.888
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae D39

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae R6

88.179

100

0.882
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

88.179

100

0.882
  comYA Streptococcus gordonii str. Challis substr. CH1

77.419

99.042

0.767
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.042

0.597
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

44.231

83.067

0.367