NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	P8P68_RS06255	Genome accession	NZ_CP121467
Coordinates	1291040..1291501 (-)	Length	153 a.a.
NCBI ID	WP_049490202.1	Uniprot ID	A0AAX3TJD5
Organism	Streptococcus sp. D7B5
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1286040..1296501

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P8P68_RS06230 (P8P68_06230)	comYH	1286800..1287753 (-)	954	WP_278275775.1	class I SAM-dependent methyltransferase	Machinery gene
P8P68_RS06235 (P8P68_06235)	-	1288024..1288611 (+)	588	WP_084851076.1	Ltp family lipoprotein	-
P8P68_RS06240 (P8P68_06240)	-	1288685..1289996 (+)	1312	Protein_1197	IS3 family transposase	-
P8P68_RS06245 (P8P68_06245)	-	1289990..1290616 (-)	627	WP_278275776.1	class I SAM-dependent methyltransferase	-
P8P68_RS06250 (P8P68_06250)	comGG/cglG	1290649..1291062 (-)	414	WP_049490203.1	competence type IV pilus minor pilin ComGG	Machinery gene
P8P68_RS06255 (P8P68_06255)	comGF/cglF	1291040..1291501 (-)	462	WP_049490202.1	competence type IV pilus minor pilin ComGF	Machinery gene
P8P68_RS06260 (P8P68_06260)	comGE/cglE	1291464..1291766 (-)	303	WP_000413367.1	competence type IV pilus minor pilin ComGE	Machinery gene
P8P68_RS06265 (P8P68_06265)	comGD/cglD	1291729..1292163 (-)	435	WP_049519116.1	competence type IV pilus minor pilin ComGD	Machinery gene
P8P68_RS06270 (P8P68_06270)	comGC/cglC	1292126..1292449 (-)	324	WP_000735798.1	competence type IV pilus major pilin ComGC	Machinery gene
P8P68_RS06275 (P8P68_06275)	comGB/cglB	1292451..1293467 (-)	1017	WP_000355379.1	competence type IV pilus assembly protein ComGB	Machinery gene
P8P68_RS06280 (P8P68_06280)	comGA/cglA/cilD	1293415..1294356 (-)	942	WP_000249575.1	competence type IV pilus ATPase ComGA	Machinery gene
P8P68_RS06285 (P8P68_06285)	-	1294426..1294791 (-)	366	WP_000286396.1	DUF1033 family protein	-
P8P68_RS06290 (P8P68_06290)	nagA	1294946..1296097 (-)	1152	WP_001134447.1	N-acetylglucosamine-6-phosphate deacetylase	-

Sequence

Protein

Download Length: 153 a.a. Molecular weight: 17863.56 Da Isoelectric Point: 9.9136

                                >NTDB_id=812232 P8P68_RS06255 WP_049490202.1 1291040..1291501(-) (comGF/cglF) [Streptococcus sp. D7B5]

MVQRSYSTLKSSKVRAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQKQSEQKEWLLFVDQLEVELERSQFEKVEGN

RLYMKQDGKDISIGKSKSDDFRKTDTSGRGYQPMVYGLKSAQITEENQLVRFRFQFQKGLEREFIYRVEKAKS

Nucleotide

Download Length: 462 bp

                                >NTDB_id=812232 P8P68_RS06255 WP_049490202.1 1291040..1291501(-) (comGF/cglF) [Streptococcus sp. D7B5]

ATGGTTCAGAGAAGTTACTCGACCTTAAAGAGCAGTAAGGTAAGAGCGTTCACTCTTTTAGAATCTCTGATTGCCCTTAT

CGTCATTAGCGGAGGCTTGCTCCTCTTCCAGGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGTTACCAGAAGCAAAGCG

AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTGGAGGTAGAGTTAGAGCGTTCGCAGTTTGAAAAGGTAGAAGGCAAT

CGCCTCTACATGAAACAAGATGGTAAGGATATCTCTATAGGGAAGTCTAAATCGGATGATTTTCGGAAGACCGATACCAG

CGGACGGGGCTACCAACCTATGGTTTATGGACTCAAATCAGCACAGATTACAGAGGAAAATCAATTGGTTCGCTTTCGTT

TCCAATTTCAAAAGGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGCAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus mitis SK321	89.542	100	0.895
comGF/cglF	Streptococcus mitis NCTC 12261	88.235	100	0.882
comGF/cglF	Streptococcus pneumoniae Rx1	87.582	100	0.876
comGF/cglF	Streptococcus pneumoniae D39	87.582	100	0.876
comGF/cglF	Streptococcus pneumoniae R6	87.582	100	0.876
comGF/cglF	Streptococcus pneumoniae TIGR4	87.582	100	0.876
comGF	Lactococcus lactis subsp. cremoris KW2	40	91.503	0.366

Detailed information NTG0812232