Detailed information    

experimental Experimentally validated

Overview

Name   kpsS   Type   Regulator
Locus tag   Cj1413c Genome accession   NC_002163
Coordinates   1345102..1346286 (-) Length   394 a.a.
NCBI ID   YP_002344796.1    Uniprot ID   Q0P8K1
Organism   Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819     
Function   repress natural transformation   
Competence regulation

Function

Loss of CPS and truncation of LOS increased the transformation frequency by 4- and 25-fold, respectively, and mutation of both CPS and LOS resulted in a 97-fold increase in the transformation frequency. Consistent with the increased transformation frequencies, the CPS and LOS mutants showed enhanced rates of DNA uptake.


Genomic Context

Location: 1340102..1351286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cj1408 fliL 1341250..1341786 (+) 537 YP_002344791.1 flagellar basal body-associated protein FliL -
  Cj1409 acpS 1341786..1342133 (+) 348 YP_002344792.1 holo-ACP synthase -
  Cj1410c - 1342134..1342553 (-) 420 YP_002344793.1 membrane protein -
  Cj1411c - 1342550..1343911 (-) 1362 YP_002344794.1 cytochrome P450 -
  Cj1412c - 1343908..1345029 (-) 1122 YP_002344795.1 integral membrane protein -
  Cj1413c kpsS 1345102..1346286 (-) 1185 YP_002344796.1 capsule polysaccharide modification protein Regulator
  Cj1414c kpsC 1346283..1348352 (-) 2070 YP_002344797.1 capsule polysaccharide modification protein -
  Cj1415c cysC 1348349..1348861 (-) 513 YP_002344798.1 adenylyl-sulfate kinase -
  Cj1416c - 1348851..1349612 (-) 762 YP_002344799.1 sugar nucleotidyltransferase -
  Cj1417c - 1349614..1350216 (-) 603 YP_002344800.1 amidotransferase -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 46938.68 Da        Isoelectric Point: 9.9831

>NTDB_id=1240 Cj1413c YP_002344796.1 1345102..1346286(-) (kpsS) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
MRFSTKIKKEFSGKNVLLLQGPVGNFFHHLAVKMRKNQTKVFKLNFNGGDFFFYPSGTRCKCDEKDLENFYRDFFQNKKI
DAILMYNDCRIIHAKAIKVAKELGIEIWIFEEGYLRPYCITLEKDGVNANSSLPRDKNFYLSQNIFTKESIKEIPGGFKF
MAFDAFLYWLFAFILAPFFNNKLHHRTLYPFEFLFWFRSLYRKYLYKITEKKLNEKIYNLEKKYFLAILQVYSDTQIKYH
YKKSIEHFIEETILSFANHARAKSYLVFKHHPMDRGYKNYFKLINDLSRKYHVEGRVLYVHDTHLPVLLRKALGCITINS
TVGLSAILEGCPTKVCGNAFYNFEGLSYPKKLQFFWREAHAYKPNPVLVCNFKKYLLQTNQFNGNFYKNFFLDK

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=1240 Cj1413c YP_002344796.1 1345102..1346286(-) (kpsS) [Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819]
ATGAGATTTAGCACTAAAATTAAAAAAGAATTTAGCGGTAAAAATGTTTTACTCTTGCAAGGGCCTGTGGGAAATTTTTT
TCATCATCTTGCAGTGAAAATGAGAAAAAACCAAACCAAAGTTTTTAAACTCAATTTTAACGGCGGAGATTTTTTTTTCT
ATCCTAGCGGAACGAGGTGTAAGTGTGATGAAAAAGATCTTGAAAATTTCTATAGAGATTTTTTTCAAAATAAAAAAATC
GATGCTATTTTAATGTATAATGATTGTCGCATTATACATGCTAAGGCTATTAAAGTGGCTAAAGAATTAGGAATTGAAAT
ATGGATTTTTGAAGAAGGATATTTAAGGCCTTATTGTATTACTTTGGAAAAAGATGGGGTGAATGCAAATTCTTCTTTAC
CGCGTGATAAAAATTTTTATCTTTCTCAAAATATTTTTACCAAAGAAAGCATAAAAGAAATTCCAGGTGGATTTAAATTT
ATGGCTTTTGATGCTTTTTTGTATTGGTTGTTTGCTTTTATTTTGGCTCCATTTTTTAACAACAAACTTCATCATCGCAC
TTTATATCCTTTTGAATTTTTATTTTGGTTTAGATCATTGTATAGAAAGTATCTTTATAAGATTACAGAAAAAAAACTAA
ATGAAAAGATTTATAATTTAGAAAAAAAGTACTTTTTGGCGATTTTACAAGTTTATAGCGATACGCAAATTAAATATCAT
TACAAAAAAAGCATAGAGCACTTTATAGAGGAAACCATACTTTCTTTTGCAAACCATGCAAGAGCTAAATCTTATCTAGT
TTTTAAACACCATCCCATGGATAGGGGCTATAAGAATTATTTTAAACTTATAAATGATTTAAGTCGAAAATATCATGTAG
AAGGAAGGGTTTTATATGTTCATGATACCCACTTGCCAGTTCTTTTAAGAAAGGCTTTGGGTTGTATTACTATAAATTCA
ACAGTAGGCCTTAGTGCTATTTTAGAAGGCTGTCCGACTAAGGTTTGTGGCAATGCTTTTTATAATTTTGAAGGCTTATC
TTATCCAAAAAAACTTCAATTTTTTTGGCGTGAAGCTCATGCTTATAAACCCAATCCTGTTTTGGTATGTAATTTTAAGA
AATATCTTTTACAAACTAATCAATTCAACGGCAATTTTTATAAAAATTTCTTTTTAGACAAATAA

Domains

Predicted by InterproScan.

Capsule polysaccharide biosynthesis protein (Capsule_synth)

(81-387)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0P8K1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Byeonghwa Jeon et al. (2009) Roles of lipooligosaccharide and capsular polysaccharide in antimicrobial resistance and natural transformation of Campylobacter jejuni. The Journal of Antimicrobial Chemotherapy 63(3):462-8. [PMID: 19147521]