Detailed information NTG0809324

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLHP_RS03405
• Genome accession: NZ_CP120921
• Coordinates: 666826..667296 (+)
• Length: 156 a.a.
• NCBI ID: WP_231099719.1
• Uniprot ID: -
• Organism: Lactococcus cremoris subsp. cremoris HP
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 661826..672296

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLHP_RS03385 (LLHP_03400)	-	663112..663672 (+)	561	WP_032950467.1	GNAT family N-acetyltransferase	-
LLHP_RS03390 (LLHP_03405)	-	663789..664187 (+)	399	WP_011676775.1	hypothetical protein	-
LLHP_RS03395 (LLHP_03410)	-	664293..665225 (+)	933	WP_032950468.1	ABC transporter ATP-binding protein	-
LLHP_RS03400 (LLHP_03415)	-	665222..666583 (+)	1362	WP_032950469.1	ABC transporter permease	-
LLHP_RS03405 (LLHP_03420)	comEA	666826..667296 (+)	471	WP_231099719.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLHP_RS03410 (LLHP_03425)	comEC	667424..668695 (+)	1272	WP_324187043.1	ComEC/Rec2 family competence protein	Machinery gene
LLHP_RS03415 (LLHP_03430)	comEC	668692..669201 (+)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LLHP_RS03420 (LLHP_03435)	-	669278..670168 (+)	891	WP_015082326.1	IS982-like element IS982B family transposase	-
LLHP_RS03425 (LLHP_03440)	-	670189..670494 (+)	306	Protein_665	ComEC/Rec2 family competence protein	-
LLHP_RS03430 (LLHP_03445)	-	670778..671554 (+)	777	WP_014572211.1	alpha/beta hydrolase family protein	-
LLHP_RS03435 (LLHP_03450)	-	671737..671952 (+)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-

Sequence

Protein

• Length: 156 a.a.
• Molecular weight: 17024.20 Da
• Isoelectric Point: 5.0572

>NTDB_id=809324 LLHP_RS03405 WP_231099719.1 666826..667296(+) (comEA) [Lactococcus cremoris subsp. cremoris HP]
MKKNKNEIMVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPIS
SSVSNQVSTTSGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 471 bp

>NTDB_id=809324 LLHP_RS03405 WP_231099719.1 666826..667296(+) (comEA) [Lactococcus cremoris subsp. cremoris HP]
GTGAAAAAAAATAAAAACGAAATTATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGA
TGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGA
AACTTAAAGATGAAGAAGTGATTTATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTATTAGC
AGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAA
ACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGG
ATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (92-153)

• SLBB domain (SLBB) (9-64)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	96.129	99.359	0.955
comEA/celA/cilE	Streptococcus mitis SK321	51.007	95.513	0.487
comEA	Latilactobacillus sakei subsp. sakei 23K	44.512	100	0.468
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.993	95.513	0.468
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.322	95.513	0.462
comEA/celA/cilE	Streptococcus pneumoniae D39	48.322	95.513	0.462
comEA/celA/cilE	Streptococcus pneumoniae R6	48.322	95.513	0.462
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.651	95.513	0.455
comEA	Streptococcus thermophilus LMD-9	42.5	100	0.436
comEA	Staphylococcus aureus N315	41.83	98.077	0.41
comEA	Staphylococcus aureus MW2	41.83	98.077	0.41
comEA	Bacillus subtilis subsp. subtilis str. 168	41.216	94.872	0.391