Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RBH03_RS24060 Genome accession   NZ_CP132995
Coordinates   5180235..5180699 (+) Length   154 a.a.
NCBI ID   WP_031630343.1    Uniprot ID   Q93PL5
Organism   Pseudomonas aeruginosa strain ZYPA192     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5175235..5185699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH03_RS24040 (RBH03_24045) pilC 5175659..5176876 (-) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  RBH03_RS24045 (RBH03_24050) pilB 5176880..5177974 (-) 1095 Protein_4750 type IV-A pilus assembly ATPase PilB -
  RBH03_RS24050 (RBH03_24055) - 5178043..5179235 (+) 1193 WP_257713843.1 IS3-like element ISPpu29 family transposase -
  RBH03_RS24055 (RBH03_24060) - 5179388..5180005 (-) 618 Protein_4752 type IV-A pilus assembly ATPase PilB -
  RBH03_RS24060 (RBH03_24065) pilA 5180235..5180699 (+) 465 WP_031630343.1 pilin Machinery gene
  RBH03_RS24070 (RBH03_24075) nadC 5180902..5181750 (-) 849 WP_096253229.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RBH03_RS24075 (RBH03_24080) - 5182036..5184291 (+) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  RBH03_RS24080 (RBH03_24085) ampD 5184442..5185008 (+) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15780.91 Da        Isoelectric Point: 6.1571

>NTDB_id=797873 RBH03_RS24060 WP_031630343.1 5180235..5180699(+) (pilA) [Pseudomonas aeruginosa strain ZYPA192]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVSRSQVSRVMAEAGSLKTAVEACLQDGRTAVGTAAGQCDPGATGSSL
LTGASQTSQTLPTNTGVPQVLDPLTTQTTIIATFGNGASAAISGQTLTWTRDVNGGWSCATTVDAKFRPNGCTD

Nucleotide


Download         Length: 465 bp        

>NTDB_id=797873 RBH03_RS24060 WP_031630343.1 5180235..5180699(+) (pilA) [Pseudomonas aeruginosa strain ZYPA192]
ATGAAAGCACAGAAGGGTTTTACTCTGATTGAACTGATGATCGTAGTTGCGATCATTGGTATTCTGGCTGCAATCGCAAT
TCCCCAATATCAGAACTATGTTTCTCGCTCTCAGGTCTCCAGGGTTATGGCAGAGGCTGGCTCCTTGAAGACTGCAGTTG
AGGCCTGCCTCCAGGATGGTCGTACTGCTGTGGGTACTGCTGCTGGTCAATGCGACCCGGGTGCGACGGGTTCCAGTTTG
TTGACTGGTGCTTCTCAGACTTCTCAAACCCTGCCAACCAATACCGGTGTTCCGCAGGTTCTGGATCCTCTGACTACTCA
AACCACTATCATTGCGACTTTTGGTAACGGTGCATCCGCAGCTATTTCTGGCCAGACTCTGACCTGGACTCGTGATGTTA
ATGGTGGCTGGAGCTGTGCTACTACCGTAGATGCTAAATTCCGTCCTAATGGCTGTACTGACTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q93PL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.753

100

0.468
  pilA Vibrio cholerae strain A1552

43.59

100

0.442
  pilA Vibrio cholerae C6706

43.59

100

0.442
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.59

100

0.442
  comP Acinetobacter baylyi ADP1

40.12

100

0.435
  pilA Vibrio parahaemolyticus RIMD 2210633

40.881

100

0.422
  pilA Acinetobacter baumannii strain A118

41.83

99.351

0.416
  pilA2 Legionella pneumophila strain ERS1305867

41.558

100

0.416
  pilA2 Legionella pneumophila str. Paris

40.909

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.403
  pilE Neisseria gonorrhoeae MS11

33.908

100

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.411

98.052

0.377