Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PWW26_RS03430 Genome accession   NZ_CP118596
Coordinates   687013..687462 (-) Length   149 a.a.
NCBI ID   WP_050922072.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 682013..692462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW26_RS03410 coaE 682513..683127 (-) 615 WP_017817675.1 dephospho-CoA kinase -
  PWW26_RS03415 pilD 683128..683997 (-) 870 WP_274878547.1 A24 family peptidase Machinery gene
  PWW26_RS03420 pilC 684070..685293 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW26_RS03425 pilB 685327..687012 (-) 1686 WP_017817677.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW26_RS03430 pilA 687013..687462 (-) 450 WP_050922072.1 pilin Machinery gene
  PWW26_RS03435 nadC 687727..688614 (-) 888 WP_274878548.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW26_RS03440 ampD 688707..689258 (+) 552 WP_017189135.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW26_RS03445 pdhR 689663..690430 (+) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15593.74 Da        Isoelectric Point: 7.7823

>NTDB_id=793799 PWW26_RS03430 WP_050922072.1 687013..687462(-) (pilA) [Vibrio harveyi strain fish10]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGAGLATITSVRTNVEDYIVTNGSFPDGTTSGQLTTD
LGVIQPNNGTITFDDTNGNILLTFAGTGNSPDINSAKIALNRAAGGTWTCKATPATKLLPKSCTQDSSL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=793799 PWW26_RS03430 WP_050922072.1 687013..687462(-) (pilA) [Vibrio harveyi strain fish10]
ATGAAAACGAATAAACAGAAGAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATCGTGGTAGCGGTTATTGGTGTTTT
GGCAGCGATTGCAATTCCTCAATATCAAAACTATGTTGCAAAGTCTGAACTTGGTGCAGGACTTGCAACAATAACTTCAG
TTAGAACTAACGTTGAAGATTATATTGTTACAAATGGCAGTTTTCCTGATGGTACAACATCGGGACAATTAACAACTGAT
TTAGGTGTAATTCAACCAAACAACGGTACCATCACCTTTGATGATACGAACGGGAATATTTTGTTAACTTTCGCTGGTAC
AGGTAACAGTCCTGATATAAACAGCGCGAAAATCGCTCTTAACAGAGCCGCGGGCGGTACGTGGACGTGTAAAGCAACTC
CAGCAACTAAGCTCCTTCCAAAGTCATGCACGCAAGATTCTAGCCTGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.592

100

0.544
  pilA Vibrio cholerae strain A1552

51.592

100

0.544
  pilA Vibrio cholerae C6706

51.592

100

0.544
  pilA Pseudomonas aeruginosa PAK

44.295

100

0.443
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.069

97.315

0.409
  pilA Vibrio parahaemolyticus RIMD 2210633

46.923

87.248

0.409
  pilA Acinetobacter baumannii strain A118

43.382

91.275

0.396
  pilA/pilA1 Eikenella corrodens VA1

33.951

100

0.369
  pilA Haemophilus influenzae 86-028NP

36

100

0.362
  pilA Acinetobacter nosocomialis M2

36.986

97.987

0.362