Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PWW26_RS03425 Genome accession   NZ_CP118596
Coordinates   685327..687012 (-) Length   561 a.a.
NCBI ID   WP_017817677.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 680327..692012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW26_RS03395 rplS 680768..681121 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  PWW26_RS03400 yacG 681488..681688 (-) 201 WP_026000212.1 DNA gyrase inhibitor YacG -
  PWW26_RS03405 zapD 681745..682485 (-) 741 WP_274878546.1 cell division protein ZapD -
  PWW26_RS03410 coaE 682513..683127 (-) 615 WP_017817675.1 dephospho-CoA kinase -
  PWW26_RS03415 pilD 683128..683997 (-) 870 WP_274878547.1 A24 family peptidase Machinery gene
  PWW26_RS03420 pilC 684070..685293 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  PWW26_RS03425 pilB 685327..687012 (-) 1686 WP_017817677.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWW26_RS03430 pilA 687013..687462 (-) 450 WP_050922072.1 pilin Machinery gene
  PWW26_RS03435 nadC 687727..688614 (-) 888 WP_274878548.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PWW26_RS03440 ampD 688707..689258 (+) 552 WP_017189135.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PWW26_RS03445 pdhR 689663..690430 (+) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62000.80 Da        Isoelectric Point: 5.2849

>NTDB_id=793798 PWW26_RS03425 WP_017817677.1 685327..687012(-) (pilB) [Vibrio harveyi strain fish10]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLSSGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFKELSAAIRRLYGRALGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDHQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKTEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPHCRLPQEPNSQLQHIGITSTED
IFQANPDGCNECTQGYSGRTGIYEVMKFDESLSNALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=793798 PWW26_RS03425 WP_017817677.1 685327..687012(-) (pilB) [Vibrio harveyi strain fish10]
ATGCTAACCAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
TAAAGCTTCTGGTATTTCGATGCCGGAGGCTTTGCTCAGTTCTGGCTTTTTCCAATCACATGAGCTAACTGAGCACCTTA
GCTCTATCTTTGGGCTTTCCTGCACATCACTCAGTCAGTATGAATACTCGTCGCTGTGCCAAAAGCTTGGCTTGCGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCGACATTAATACTCGCGGTTGCAGACCCAACTAACTT
GCAAGCGGAAGATGACTTTCGTTTTGCTACCGGTTTACAAGTCGAGCTAGTTCTGGCGGATTTTAAAGAACTCAGCGCTG
CCATTCGTCGTTTATATGGTCGTGCACTCGGTCAAGAAAAGTCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCAGGT
CTCGTTGATATTGGTGAGGACGAAATTGAGAACATTGAAGATCTCAGCCAAGATGAATCGCCTGTCAGTCGTTATATTAA
CCAAATTCTGCTCGACGCCGTTCGTAAAGGGGCTTCCGATATTCACTTCGAGCCCTATGAAAACATGTACCGAGTGCGTT
TGCGTTGCGATGGCATCTTGATCGAAATTCAGCAGCCTCCGAGCCATTTAAGCCGTCGCCTCTCAGCCCGAATAAAGATC
CTATCTAAGCTTGATATCGCTGAGCGCCGTTTACCACAGGATGGTCGCATCAAGCTCAAATTAAACCAAGACACCGCGAT
TGATATGCGCGTTTCTACACTGCCAACTCTGTTTGGGGAGAAAATCGTACTACGCTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAACTCGGATACAGCGATCATCAAAAGCAATTGTATTTAGAAGCACTGCGCCGCCCACAAGGCATGATC
CTAATGACAGGACCAACGGGCAGCGGCAAAACGGTTTCTCTTTACACTGGTTTGAGCATTCTCAATAAAACCGAGATCAA
CATTTCCACCGCTGAAGACCCCGTTGAGATCAACTTACCCGGCATTAACCAAGTTCAAGTTCAACCTAAGATCGGCTTTG
GCTTTGCAGAAGCGCTTCGCTCTTTCTTGCGTCAAGATCCCGATGTAGTCATGGTGGGTGAGATCCGAGATCTCGACACG
GCAGAAATCGCGATCAAAGCGTCACAAACTGGACACTTAGTCCTTTCTACCCTGCATACCAACTCCGCCGCCGAGACGGT
TATTCGCTTATCAAATATGGGGGTAGAAAGCTTTAACCTCGCATCCTCTCTTAGTTTGATCATCGCTCAGCGTTTAGCAC
GAAAACTTTGTCCACATTGCAGACTACCTCAAGAACCAAACAGCCAACTTCAACATATTGGAATTACCAGCACTGAAGAT
ATCTTCCAAGCGAACCCTGACGGTTGTAACGAATGCACCCAAGGATACTCTGGTCGCACAGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTGTCAAATGCGCTCATCAAAGGTGCTTCAGTGCATGAATTAGAAAAGCTAGCGATTGCCAATGGTA
TGCAGACTCTACAAATGTCCGGCATTGAAAAGCTCAAACAAGGCGTCACCAGCTTTAGTGAGCTGCAACGCGTTCTCTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

96.257

100

0.963
  pilB Vibrio parahaemolyticus RIMD 2210633

89.661

100

0.897
  pilB Vibrio cholerae strain A1552

73.132

100

0.733
  pilB Acinetobacter baylyi ADP1

48.294

99.287

0.479
  pilB Acinetobacter baumannii D1279779

51.838

92.157

0.478
  pilB Legionella pneumophila strain ERS1305867

48.339

96.613

0.467
  pilF Neisseria gonorrhoeae MS11

43.06

100

0.431
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.738

99.465

0.365
  pilF Thermus thermophilus HB27

36.299

100

0.364