Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA/pilAI   Type   Machinery gene
Locus tag   PWE35_RS16475 Genome accession   NZ_CP118547
Coordinates   3540849..3541256 (-) Length   135 a.a.
NCBI ID   WP_024956772.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain HT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3535849..3546256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWE35_RS16465 (PWE35_16465) pilR 3537392..3538765 (+) 1374 WP_005410795.1 sigma-54 dependent transcriptional regulator Regulator
  PWE35_RS16470 (PWE35_16470) pilB 3538892..3540625 (-) 1734 WP_005414305.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PWE35_RS16475 (PWE35_16475) pilA/pilAI 3540849..3541256 (-) 408 WP_024956772.1 pilin Machinery gene
  PWE35_RS16480 (PWE35_16480) - 3541409..3541846 (-) 438 WP_024956771.1 pilin -
  PWE35_RS16485 (PWE35_16485) pilC 3542199..3543458 (+) 1260 WP_049452341.1 type II secretion system F family protein Machinery gene
  PWE35_RS16490 (PWE35_16490) - 3543466..3544329 (+) 864 WP_005410800.1 A24 family peptidase -
  PWE35_RS16495 (PWE35_16495) coaE 3544341..3544952 (+) 612 WP_164225734.1 dephospho-CoA kinase -
  PWE35_RS16500 (PWE35_16500) - 3545287..3546201 (+) 915 WP_005407612.1 IS110-like element ISStma6 family transposase -

Sequence


Protein


Download         Length: 135 a.a.        Molecular weight: 14252.43 Da        Isoelectric Point: 6.2109

>NTDB_id=793257 PWE35_RS16475 WP_024956772.1 3540849..3541256(-) (pilA/pilAI) [Stenotrophomonas maltophilia strain HT2]
MNTQKGFTLIELMIVVAIIAILAAIAAVAFQPYVVRTQLAAALADITPGKTKVEILVNENRPASLITPEFIGGAQNAHCS
NVEAELSDSGVGHISCTLKGHSALDGKDLILRRSAEGIWSCDGSAFEARYRPTGC

Nucleotide


Download         Length: 408 bp        

>NTDB_id=793257 PWE35_RS16475 WP_024956772.1 3540849..3541256(-) (pilA/pilAI) [Stenotrophomonas maltophilia strain HT2]
ATGAACACGCAGAAGGGCTTCACGCTCATCGAACTGATGATCGTGGTGGCCATCATCGCCATCCTTGCGGCGATTGCGGC
TGTGGCATTTCAGCCATACGTCGTGCGCACCCAGTTGGCGGCGGCACTTGCGGATATCACGCCGGGAAAGACCAAGGTGG
AAATCCTCGTCAACGAGAACCGTCCGGCCAGTCTGATCACCCCCGAGTTCATCGGTGGCGCCCAGAACGCCCACTGCTCC
AACGTCGAAGCCGAACTCAGCGATTCCGGCGTCGGCCATATCAGCTGCACCTTGAAGGGACACAGCGCGCTGGACGGCAA
GGACCTGATCCTGCGCCGCTCCGCCGAGGGCATCTGGAGCTGCGATGGCAGCGCGTTCGAAGCGCGCTATCGCCCGACCG
GCTGCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.735

100

0.511

  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.382

100

0.437

  pilA Pseudomonas aeruginosa PAK

38.255

100

0.422

  pilA Acinetobacter baumannii strain A118

40.876

100

0.415

  comP Acinetobacter baylyi ADP1

35.57

100

0.393

  pilA Vibrio cholerae C6706

34.694

100

0.378

  pilA Vibrio cholerae strain A1552

34.694

100

0.378

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.694

100

0.378

  pilA Ralstonia pseudosolanacearum GMI1000

36.842

98.519

0.363

  pilA2 Legionella pneumophila strain ERS1305867

35.507

100

0.363