Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PTC91_RS25505 Genome accession   NZ_CP117749
Coordinates   5387428..5387880 (-) Length   150 a.a.
NCBI ID   WP_031636800.1    Uniprot ID   P17837
Organism   Pseudomonas aeruginosa strain 2022CK-00828     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 5381946..5388956 5387428..5387880 within 0


Gene organization within MGE regions


Location: 5381946..5388956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTC91_RS25480 (PTC91_25480) - 5381946..5383241 (+) 1296 WP_006226034.1 ISL3 family transposase -
  PTC91_RS25485 (PTC91_25485) ampD 5383276..5383689 (-) 414 Protein_5045 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PTC91_RS25490 (PTC91_25490) - 5383840..5386095 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  PTC91_RS25495 (PTC91_25495) nadC 5386381..5387229 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PTC91_RS25505 (PTC91_25505) pilA 5387428..5387880 (-) 453 WP_031636800.1 pilin Machinery gene
  PTC91_RS25510 (PTC91_25510) - 5387976..5388956 (+) 981 WP_010466230.1 IS5-like element IS1384 family transposase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15743.11 Da        Isoelectric Point: 8.4742

>NTDB_id=787391 PTC91_RS25505 WP_031636800.1 5387428..5387880(-) (pilA) [Pseudomonas aeruginosa strain 2022CK-00828]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTVKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=787391 PTC91_RS25505 WP_031636800.1 5387428..5387880(-) (pilA) [Pseudomonas aeruginosa strain 2022CK-00828]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAAGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P17837

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

66.447

100

0.673
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45

100

0.48
  pilA Vibrio cholerae strain A1552

45

100

0.48
  pilA Vibrio cholerae C6706

45

100

0.48
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46

100

0.46
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilA1 Eikenella corrodens VA1

39.865

98.667

0.393
  pilA2 Legionella pneumophila str. Paris

38.255

99.333

0.38
  pilA2 Legionella pneumophila strain ERS1305867

38.255

99.333

0.38