Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IL310_RS02865 Genome accession   NZ_CP117409
Coordinates   173807..174745 (+) Length   312 a.a.
NCBI ID   WP_058206479.1    Uniprot ID   -
Organism   Lactococcus lactis strain LB7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 168807..179745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL310_RS02865 (IL310_02870) comGA 173807..174745 (+) 939 WP_058206479.1 competence type IV pilus ATPase ComGA Machinery gene
  IL310_RS02870 (IL310_02875) comGB 174639..175712 (+) 1074 WP_201248649.1 competence type IV pilus assembly protein ComGB Machinery gene
  IL310_RS02875 (IL310_02880) comGC 175726..176109 (+) 384 WP_012898622.1 competence type IV pilus major pilin ComGC Machinery gene
  IL310_RS02880 (IL310_02885) comGD 176069..176500 (+) 432 WP_012898621.1 competence type IV pilus minor pilin ComGD Machinery gene
  IL310_RS02885 (IL310_02890) comGE 176472..176768 (+) 297 WP_033900709.1 competence type IV pilus minor pilin ComGE Machinery gene
  IL310_RS02890 (IL310_02895) comGF 176731..177177 (+) 447 WP_201248646.1 competence type IV pilus minor pilin ComGF Machinery gene
  IL310_RS02895 (IL310_02900) comGG 177216..177500 (+) 285 WP_025017139.1 competence type IV pilus minor pilin ComGG Machinery gene
  IL310_RS02900 (IL310_02905) - 177582..178019 (+) 438 WP_201248645.1 zinc-dependent MarR family transcriptional regulator -
  IL310_RS02905 (IL310_02910) - 178016..178858 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35649.84 Da        Isoelectric Point: 6.1811

>NTDB_id=784617 IL310_RS02865 WP_058206479.1 173807..174745(+) (comGA) [Lactococcus lactis strain LB7]
MIPKMAKELIQRAIDRGASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=784617 IL310_RS02865 WP_058206479.1 173807..174745(+) (comGA) [Lactococcus lactis strain LB7]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGGTGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGACTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.244

100

0.772
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.698

99.679

0.535
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.733

99.679

0.526
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.733

99.679

0.526
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.733

99.679

0.526
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.733

99.679

0.526
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519