Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   PML25_RS06095 Genome accession   NZ_CP116672
Coordinates   1465558..1465983 (+) Length   141 a.a.
NCBI ID   WP_168958950.1    Uniprot ID   A0A6V7CDJ8
Organism   Xanthomonas hortorum pv. pelargonii strain 305     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1416869..1493280 1465558..1465983 within 0


Gene organization within MGE regions


Location: 1416869..1493280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML25_RS05885 (PML25_05885) - 1418269..1418724 (+) 456 WP_168960054.1 hypothetical protein -
  PML25_RS05890 (PML25_05890) - 1418721..1422404 (+) 3684 WP_233366659.1 peptidoglycan-binding protein -
  PML25_RS05900 (PML25_05900) - 1423912..1425210 (+) 1299 WP_272164465.1 HlyD family efflux transporter periplasmic adaptor subunit -
  PML25_RS05905 (PML25_05905) - 1425207..1427423 (+) 2217 WP_168960052.1 peptidase domain-containing ABC transporter -
  PML25_RS05910 (PML25_05910) - 1427530..1428342 (+) 813 WP_006450247.1 hypothetical protein -
  PML25_RS05915 (PML25_05915) - 1428382..1428906 (+) 525 WP_168960051.1 hypothetical protein -
  PML25_RS05920 (PML25_05920) - 1429056..1429943 (-) 888 Protein_1169 ABC transporter permease -
  PML25_RS05925 (PML25_05925) - 1430018..1431126 (+) 1109 WP_256627460.1 IS3 family transposase -
  PML25_RS05930 (PML25_05930) - 1431145..1431537 (-) 393 Protein_1171 ABC transporter permease -
  PML25_RS05935 (PML25_05935) - 1431600..1432031 (-) 432 WP_168960049.1 toxin-activating lysine-acyltransferase -
  PML25_RS05940 (PML25_05940) - 1432021..1433835 (-) 1815 WP_168960048.1 protein adenylyltransferase SelO family protein -
  PML25_RS23200 - 1434351..1434887 (+) 537 Protein_1174 MobA/MobL family protein -
  PML25_RS23205 - 1434786..1435007 (-) 222 Protein_1175 hypothetical protein -
  PML25_RS05950 (PML25_05950) - 1436341..1437321 (-) 981 WP_256627476.1 IS5 family transposase -
  PML25_RS05955 (PML25_05955) - 1437583..1438272 (+) 690 WP_233366544.1 hypothetical protein -
  PML25_RS05960 (PML25_05960) - 1438377..1439111 (-) 735 WP_168958928.1 hypothetical protein -
  PML25_RS05965 (PML25_05965) - 1439155..1439604 (-) 450 WP_168958929.1 DUF6527 family protein -
  PML25_RS05970 (PML25_05970) - 1439601..1440758 (-) 1158 WP_272164455.1 ThiF family adenylyltransferase -
  PML25_RS05975 (PML25_05975) - 1440786..1441766 (+) 981 WP_233366556.1 IS5 family transposase -
  PML25_RS05980 (PML25_05980) - 1441923..1442618 (-) 696 WP_168958931.1 multiubiquitin domain-containing protein -
  PML25_RS05985 (PML25_05985) - 1442766..1443092 (+) 327 WP_168958932.1 helix-turn-helix transcriptional regulator -
  PML25_RS05990 (PML25_05990) - 1443105..1444010 (+) 906 WP_168958933.1 ImmA/IrrE family metallo-endopeptidase -
  PML25_RS05995 (PML25_05995) - 1444272..1445486 (-) 1215 WP_168958934.1 MFS transporter -
  PML25_RS06000 (PML25_06000) - 1445483..1446676 (-) 1194 WP_168958935.1 serine hydrolase domain-containing protein -
  PML25_RS06005 (PML25_06005) - 1446790..1447710 (+) 921 WP_168958936.1 LysR family transcriptional regulator -
  PML25_RS06010 (PML25_06010) - 1447809..1448231 (+) 423 WP_168958937.1 YqaA family protein -
  PML25_RS06015 (PML25_06015) - 1448522..1450768 (-) 2247 WP_168958938.1 methyl-accepting chemotaxis protein -
  PML25_RS06020 (PML25_06020) - 1451192..1451506 (+) 315 WP_023904920.1 hypothetical protein -
  PML25_RS06025 (PML25_06025) - 1451503..1451772 (-) 270 WP_168958939.1 hypothetical protein -
  PML25_RS06030 (PML25_06030) - 1451936..1453123 (-) 1188 WP_168958940.1 hypothetical protein -
  PML25_RS06035 (PML25_06035) - 1453378..1453773 (+) 396 WP_168959001.1 hypothetical protein -
  PML25_RS06040 (PML25_06040) - 1453862..1454254 (+) 393 WP_055824272.1 H-NS family nucleoid-associated regulatory protein -
  PML25_RS06045 (PML25_06045) glgX 1454612..1456744 (-) 2133 WP_168958942.1 glycogen debranching protein GlgX -
  PML25_RS06050 (PML25_06050) rimK 1457239..1458159 (+) 921 WP_168958943.1 30S ribosomal protein S6--L-glutamate ligase -
  PML25_RS06055 (PML25_06055) - 1458503..1459180 (+) 678 WP_006448355.1 response regulator transcription factor -
  PML25_RS06060 (PML25_06060) - 1459173..1460507 (+) 1335 WP_168958944.1 HAMP domain-containing sensor histidine kinase -
  PML25_RS06065 (PML25_06065) - 1460656..1461147 (-) 492 WP_168958945.1 GNAT family N-acetyltransferase -
  PML25_RS06070 (PML25_06070) - 1461144..1461434 (-) 291 WP_168958946.1 DUF1778 domain-containing protein -
  PML25_RS06075 (PML25_06075) - 1461510..1461911 (-) 402 WP_168958947.1 SymE family type I addiction module toxin -
  PML25_RS06080 (PML25_06080) coaE 1462440..1463063 (-) 624 WP_168958948.1 dephospho-CoA kinase -
  PML25_RS06085 (PML25_06085) - 1463077..1463940 (-) 864 WP_016849610.1 A24 family peptidase -
  PML25_RS06090 (PML25_06090) pilC 1463947..1465206 (-) 1260 WP_168958949.1 type II secretion system F family protein Machinery gene
  PML25_RS06095 (PML25_06095) pilA2 1465558..1465983 (+) 426 WP_168958950.1 pilin Machinery gene
  PML25_RS06100 (PML25_06100) - 1466119..1467708 (+) 1590 WP_168958951.1 phosphoethanolamine transferase -
  PML25_RS06105 (PML25_06105) - 1467779..1468348 (+) 570 WP_233366553.1 hypothetical protein -
  PML25_RS06110 (PML25_06110) - 1468318..1469844 (+) 1527 WP_168958953.1 hypothetical protein -
  PML25_RS06115 (PML25_06115) pilB 1469879..1471615 (+) 1737 WP_168958954.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PML25_RS06120 (PML25_06120) - 1471624..1472241 (-) 618 WP_168958955.1 Fic family protein -
  PML25_RS06125 (PML25_06125) - 1472238..1472420 (-) 183 WP_074066024.1 YhfG family protein -
  PML25_RS06130 (PML25_06130) pilR 1472494..1473948 (-) 1455 WP_180336569.1 sigma-54 dependent transcriptional regulator Regulator
  PML25_RS06135 (PML25_06135) - 1474034..1475647 (-) 1614 WP_168958956.1 HAMP domain-containing sensor histidine kinase -
  PML25_RS06140 (PML25_06140) sucC 1475880..1477049 (+) 1170 WP_006450728.1 ADP-forming succinate--CoA ligase subunit beta -
  PML25_RS06145 (PML25_06145) sucD 1477074..1477949 (+) 876 WP_006450729.1 succinate--CoA ligase subunit alpha -
  PML25_RS06150 (PML25_06150) - 1478132..1479073 (-) 942 WP_168958957.1 LysR family transcriptional regulator -
  PML25_RS06155 (PML25_06155) - 1479154..1479330 (+) 177 WP_218974074.1 hypothetical protein -
  PML25_RS06160 (PML25_06160) - 1479394..1479798 (+) 405 WP_218974075.1 hypothetical protein -
  PML25_RS06165 (PML25_06165) - 1480222..1481043 (+) 822 WP_233366546.1 MBL fold metallo-hydrolase -
  PML25_RS06170 (PML25_06170) - 1481048..1481443 (+) 396 WP_168958959.1 nuclear transport factor 2 family protein -
  PML25_RS06175 (PML25_06175) - 1481937..1483577 (+) 1641 WP_168958960.1 NAD+ synthase -
  PML25_RS06180 (PML25_06180) - 1483571..1484548 (-) 978 WP_168958961.1 hypothetical protein -
  PML25_RS06185 (PML25_06185) - 1484893..1485774 (-) 882 WP_168958962.1 outer membrane protein assembly factor BamD -
  PML25_RS06190 (PML25_06190) rluD 1485894..1486889 (+) 996 WP_168958963.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PML25_RS06195 (PML25_06195) pgeF 1486891..1487706 (+) 816 WP_168958964.1 peptidoglycan editing factor PgeF -
  PML25_RS06200 (PML25_06200) - 1487709..1488233 (+) 525 WP_168958965.1 DUF4166 domain-containing protein -
  PML25_RS06205 (PML25_06205) - 1488349..1488774 (+) 426 WP_168958966.1 thiol-disulfide oxidoreductase DCC family protein -
  PML25_RS06210 (PML25_06210) - 1489037..1490905 (-) 1869 WP_168958967.1 methyl-accepting chemotaxis protein -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 14385.63 Da        Isoelectric Point: 9.0171

>NTDB_id=778987 PML25_RS06095 WP_168958950.1 1465558..1465983(+) (pilA2) [Xanthomonas hortorum pv. pelargonii strain 305]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETSSSLGGLAQVTAANSGYAFPGATK
YVSGIAITSATGQVAVTSTVPNATGTILLTPTDVGGGQLKWTCSSAINVKYLPAECRNSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=778987 PML25_RS06095 WP_168958950.1 1465558..1465983(+) (pilA2) [Xanthomonas hortorum pv. pelargonii strain 305]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGCGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAGCTTGCGGTAA
CTGAGACTTCATCGTCGCTCGGCGGCCTCGCTCAGGTTACTGCGGCAAATTCGGGCTACGCATTCCCGGGTGCCACGAAG
TATGTCAGCGGCATCGCCATCACCAGTGCTACGGGCCAAGTCGCTGTAACCTCCACCGTGCCGAACGCAACCGGTACCAT
CCTGCTGACCCCGACTGATGTCGGTGGTGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCAACGTCAAGTACCTGCCGG
CGGAGTGCCGTAACTCGGGCACCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6V7CDJ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

52.941

96.454

0.511

  pilA2 Legionella pneumophila strain ERS1305867

52.941

96.454

0.511

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.216

100

0.475

  pilA Ralstonia pseudosolanacearum GMI1000

39.634

100

0.461

  comP Acinetobacter baylyi ADP1

43.537

100

0.454

  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.754

97.872

0.418

  pilE Neisseria gonorrhoeae strain FA1090

37.342

100

0.418

  pilA/pilA1 Eikenella corrodens VA1

39.333

100

0.418

  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.606

97.163

0.404

  pilE Neisseria gonorrhoeae MS11

35.625

100

0.404

  pilA Pseudomonas aeruginosa PAK

35.065

100

0.383

  pilA Acinetobacter baumannii strain A118

36.111

100

0.369